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. 1974 Nov;28(5):802–806. doi: 10.1128/am.28.5.802-806.1974

Microculture Plaque Neutralization Test for California Group Arboviruses

Gary L Seawright 1,1, Gherry Harding 1, Frederick C Thomas 1,2, Robert P Hanson 1
PMCID: PMC186828  PMID: 4216288

Abstract

A microculture plaque neutralization test is described for California-group arboviruses that is as precise and quantitative as the standard test conducted in 60-mm petri dishes. It was shown that there was no significant between-panel or between-day variation in determinations and that a single pipette could be used for all serum-dilution levels within a titration without inoculum carry-over effect. The experimental protocol and statistical methods used produce 50% neutralization end points that meet the assumptions of parametric statistics. This permits the power and versatility of the analysis of variance to be exploited in testing for treatment effects in serological and immunological studies with viruses.

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Selected References

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