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. 1974 Nov;28(5):872–880. doi: 10.1128/am.28.5.872-880.1974

Direct Method for Continuous Determination of Iron Oxidation by Autotrophic Bacteria

Michael Steiner 1, Norman Lazaroff 1
PMCID: PMC186841  PMID: 4441066

Abstract

A method for direct, continuous determination of ferric ions produced in autotrophic iron oxidation, which depends upon the measurement of ferric ion absorbance at 304 nm, is described. The use of initial rates is shown to compensate for such changes in extinction during oxidation, which are due to dependence of the extinction coefficient on the ratio of complexing anions to ferric ions. A graphical method and a computer method are given for determination of absolute ferric ion concentration, at any time interval, in reaction mixtures containing Thiobacillus ferrooxidans and ferrous ions at known levels of SO42+ and hydrogen ion concentrations. Some examples are discussed of the applicability of these methods to study of the rates of ferrous ion oxidation related to sulfate concentration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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