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. 1974 Nov;28(5):897–899. doi: 10.1128/am.28.5.897-899.1974

Rapid Detection of Bovine Mycoplasma Antigens by Counterimmunoelectrophoresis

Hyun J Cho 1, Edgar V Langford 1
PMCID: PMC186848  PMID: 4474834

Abstract

Twelve reference strains of mycoplasma and acholeplasma previously reported to have been recovered from cattle were tested against hyperimmune rabbit serum by counterimmunoelectrophoresis. This technique detected antigen by the formation of precipitin lines with antibody within 1 h and promises to be a useful technique for detecting and identifying mycoplasma isolates in either pure or mixed cultures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Erno H., Jurmanová K. Bovine mycoplasmas: serological studies by double immunodiffusion, growth precipitation and growth inhibition. Acta Vet Scand. 1973;14(4):524–537. [PubMed] [Google Scholar]
  2. Erno H., Jurmanová K., Leach R. H. Bovine mycoplasmas: a serological study by the metabolic inhibition test. Acta Vet Scand. 1973;14(4):511–523. [PubMed] [Google Scholar]
  3. Gocke D. J., Howe C. Rapid detection of Australia antigen by counterimmunoelectrophoresis. J Immunol. 1970 Apr;104(4):1031–1034. [PubMed] [Google Scholar]
  4. Gourlay R. N., Leach R. H. A new mycoplasma species isolated from pneumonic lungs of calves (Mycoplasma dispar sp. nov.). J Med Microbiol. 1970 Feb;3(1):111–123. doi: 10.1099/00222615-3-1-111. [DOI] [PubMed] [Google Scholar]
  5. Langford E. V., Leach R. H. Characterization of a mycoplasma isolated from infectious bovine keratoconjunctivitis: M. bovoculi sp. nov. Can J Microbiol. 1973 Nov;19(11):1435–1444. doi: 10.1139/m73-231. [DOI] [PubMed] [Google Scholar]
  6. al-Aubaidi J. M., Fabricant J. Characterization and classification of bovine mycoplasma. Cornell Vet. 1971 Jul;61(3):490–518. [PubMed] [Google Scholar]

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