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. 1974 Dec;28(6):1040–1046. doi: 10.1128/am.28.6.1040-1046.1974

In Vitro System for Production of Mouse Mammary Tumor Virus

D L Fine 1, L O Arthur 1, J K Plowman 1, E A Hillman 1, F Klein 1
PMCID: PMC186879  PMID: 4141598

Abstract

An in vitro system for production, purification, and concentration of mouse mammary tumor virus is described. Monolayer cultures of C3H mouse mammary tumor cells propagated at 34 C in roller bottles in the presence of dexamethasone, a glucocorticoid hormone, release B-type particles which possess ribonucleic acid and a ribonucleic acid-dependent deoxyribonucleic acid polymerase. One thousandfold concentration by ultracentrifugation with subsequent gradient fractionation yielded > 7 × 1010 particles per ml in the 1.16- to 1.18-g/ml region. Mouse mammary tumor virus produced in this system was free of detectable C-type virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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