Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1995 Dec;147(6):1553–1558.

Ring chromosomes in dermatofibrosarcoma protuberans are composed of interspersed sequences from chromosomes 17 and 22.

R Naeem 1, M L Lux 1, S F Huang 1, S P Naber 1, J M Corson 1, J A Fletcher 1
PMCID: PMC1869963  PMID: 7495279

Abstract

Ring chromosomes are found in most dermatofibrosarcoma protuberans (DFSPs), and recent reports demonstrate that portions of the DFSP ring chromosomes derive from chromosome 17. In this study we characterized ring chromosomes in three DFSPs using a combined approach of karyotyping, chromosome painting, and comparative genomic hybridization. Chromosome painting demonstrated that the ring chromosomes in each DFSP were composed of discontinuous, interwoven sequences from chromosomes 17 and 22. Amplification of chromosomes 17 and 22 sequences was confirmed in each of these cases by comparative genomic hybridization, and over-representation of chromosomes 17 and 22 sequences was also demonstrated by comparative genomic hybridization in 1 of 2 cytogenetically unremarkable DFSPs. We conclude that amplification of chromosomes 17 and 22 sequences, in ring form, is a characteristic aberration in DFSP.

Full text

PDF
1553

Images in this article

1555Figure 1
on p.1555
1556Figure 2
on p.1556

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bridge J. A., Neff J. R., Sandberg A. A. Cytogenetic analysis of dermatofibrosarcoma protuberans. Cancer Genet Cytogenet. 1990 Oct 15;49(2):199–202. doi: 10.1016/0165-4608(90)90142-w. [DOI] [PubMed] [Google Scholar]
  2. Craver R. D., Correa H., Kao Y., Van Brunt T. Dermatofibrosarcoma protuberans with 46,XY,t(X;7) abnormality in a child. Cancer Genet Cytogenet. 1995 Mar;80(1):75–77. doi: 10.1016/0165-4608(95)93814-e. [DOI] [PubMed] [Google Scholar]
  3. Dal Cin P., Kools P., Sciot R., De Wever I., Van Damme B., Van de Ven W., Van den Berghe H. Cytogenetic and fluorescence in situ hybridization investigation of ring chromosomes characterizing a specific pathologic subgroup of adipose tissue tumors. Cancer Genet Cytogenet. 1993 Jul 15;68(2):85–90. doi: 10.1016/0165-4608(93)90001-3. [DOI] [PubMed] [Google Scholar]
  4. Dal Cin P., Sciot R., Aly M. S., Delabie J., Stas M., De Wever I., Van Damme B., Van den Berghe H. Some desmoid tumors are characterized by trisomy 8. Genes Chromosomes Cancer. 1994 Jun;10(2):131–135. doi: 10.1002/gcc.2870100208. [DOI] [PubMed] [Google Scholar]
  5. Fletcher J. A., Kozakewich H. P., Hoffer F. A., Lage J. M., Weidner N., Tepper R., Pinkus G. S., Morton C. C., Corson J. M. Diagnostic relevance of clonal cytogenetic aberrations in malignant soft-tissue tumors. N Engl J Med. 1991 Feb 14;324(7):436–442. doi: 10.1056/NEJM199102143240702. [DOI] [PubMed] [Google Scholar]
  6. Fletcher J. A., Naeem R., Xiao S., Corson J. M. Chromosome aberrations in desmoid tumors. Trisomy 8 may be a predictor of recurrence. Cancer Genet Cytogenet. 1995 Feb;79(2):139–143. doi: 10.1016/0165-4608(94)00134-w. [DOI] [PubMed] [Google Scholar]
  7. Heim S., Mandahl N., Kristoffersson U., Mitelman F., Röser B., Rydholm A., Willén H. Marker ring chromosome--a new cytogenetic abnormality characterizing lipogenic tumors? Cancer Genet Cytogenet. 1987 Feb;24(2):319–326. doi: 10.1016/0165-4608(87)90114-2. [DOI] [PubMed] [Google Scholar]
  8. Kallioniemi A., Kallioniemi O. P., Sudar D., Rutovitz D., Gray J. W., Waldman F., Pinkel D. Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science. 1992 Oct 30;258(5083):818–821. doi: 10.1126/science.1359641. [DOI] [PubMed] [Google Scholar]
  9. Lichter P., Tang C. J., Call K., Hermanson G., Evans G. A., Housman D., Ward D. C. High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science. 1990 Jan 5;247(4938):64–69. doi: 10.1126/science.2294592. [DOI] [PubMed] [Google Scholar]
  10. Mandahl N., Heim S., Arheden K., Rydholm A., Willén H., Mitelman F. Rings, dicentrics, and telomeric association in histiocytomas. Cancer Genet Cytogenet. 1988 Jan;30(1):23–33. doi: 10.1016/0165-4608(88)90089-1. [DOI] [PubMed] [Google Scholar]
  11. Mandahl N., Heim S., Rydholm A., Willen H., Mitelman F. Nonrandom numerical chromosome aberrations (+8, +11, +17, +20) in infantile fibrosarcoma. Cancer Genet Cytogenet. 1989 Jul 1;40(1):137–139. doi: 10.1016/0165-4608(89)90156-8. [DOI] [PubMed] [Google Scholar]
  12. Mandahl N., Heim S., Willén H., Rydholm A., Mitelman F. Supernumerary ring chromosome as the sole cytogenetic abnormality in a dermatofibrosarcoma protuberans. Cancer Genet Cytogenet. 1990 Oct 15;49(2):273–275. doi: 10.1016/0165-4608(90)90153-2. [DOI] [PubMed] [Google Scholar]
  13. Mandahl N., Höglund M., Mertens F., Rydholm A., Willén H., Brosjö O., Mitelman F. Cytogenetic aberrations in 188 benign and borderline adipose tissue tumors. Genes Chromosomes Cancer. 1994 Mar;9(3):207–215. doi: 10.1002/gcc.2870090309. [DOI] [PubMed] [Google Scholar]
  14. Mugneret F., Lizard S., Aurias A., Turc-Carel C. Chromosomes in Ewing's sarcoma. II. Nonrandom additional changes, trisomy 8 and der(16)t(1;16). Cancer Genet Cytogenet. 1988 Jun;32(2):239–245. doi: 10.1016/0165-4608(88)90286-5. [DOI] [PubMed] [Google Scholar]
  15. Orndal C., Mandahl N., Rydholm A., Willén H., Brosjö O., Heim S., Mitelman F. Supernumerary ring chromosomes in five bone and soft tissue tumors of low or borderline malignancy. Cancer Genet Cytogenet. 1992 Jun;60(2):170–175. doi: 10.1016/0165-4608(92)90011-v. [DOI] [PubMed] [Google Scholar]
  16. Pedeutour F., Coindre J. M., Nicolo G., Bouchot C., Ayraud N., Carel C. T. Ring chromosomes in dermatofibrosarcoma protuberans contain chromosome 17 sequences: fluorescence in situ hybridization. Cancer Genet Cytogenet. 1993 Jun;67(2):149–149. doi: 10.1016/0165-4608(93)90171-h. [DOI] [PubMed] [Google Scholar]
  17. Pedeutour F., Coindre J. M., Sozzi G., Nicolo G., Leroux A., Toma S., Miozzo M., Bouchot C., Hecht F., Ayraud N. Supernumerary ring chromosomes containing chromosome 17 sequences. A specific feature of dermatofibrosarcoma protuberans? Cancer Genet Cytogenet. 1994 Aug;76(1):1–9. doi: 10.1016/0165-4608(94)90060-4. [DOI] [PubMed] [Google Scholar]
  18. Pedeutour F., Simon M. P., Minoletti F., Sozzi G., Pierotti M. A., Hecht F., Turc-Carel C. Ring 22 chromosomes in dermatofibrosarcoma protuberans are low-level amplifiers of chromosome 17 and 22 sequences. Cancer Res. 1995 Jun 1;55(11):2400–2403. [PubMed] [Google Scholar]
  19. Pedeutour F., Suijkerbuijk R. F., Van Gaal J., Van de Klundert W., Coindre J. M., Van Haelst A., Collin F., Huffermann K., Turc-Carel C. Chromosome 12 origin in rings and giant markers in well-differentiated liposarcoma. Cancer Genet Cytogenet. 1993 Apr;66(2):133–134. doi: 10.1016/0165-4608(93)90245-h. [DOI] [PubMed] [Google Scholar]
  20. Sahlin P., Mark J., Stenman G. Detection of hidden structural rearrangements by FISH in pleomorphic adenomas. Genes Chromosomes Cancer. 1995 Feb;12(2):81–86. doi: 10.1002/gcc.2870120202. [DOI] [PubMed] [Google Scholar]
  21. Sankary S., Dickman P. S., Wiener E., Robichaux W., Swaney W. P., Malone P. S., Gollin S. M. Consistent numerical chromosome aberrations in congenital fibrosarcoma. Cancer Genet Cytogenet. 1993 Feb;65(2):152–156. doi: 10.1016/0165-4608(93)90225-b. [DOI] [PubMed] [Google Scholar]
  22. Schofield D. E., Fletcher J. A., Grier H. E., Yunis E. J. Fibrosarcoma in infants and children. Application of new techniques. Am J Surg Pathol. 1994 Jan;18(1):14–24. doi: 10.1097/00000478-199401000-00002. [DOI] [PubMed] [Google Scholar]
  23. Sinovic J. F., Bridge J. A., Neff J. R. Ring chromosome in parosteal osteosarcoma. Clinical and diagnostic significance. Cancer Genet Cytogenet. 1992 Aug;62(1):50–52. doi: 10.1016/0165-4608(92)90038-a. [DOI] [PubMed] [Google Scholar]
  24. Sinovic J., Bridge J. A. Translocation (2;17) in recurrent dermatofibrosarcoma protuberans. Cancer Genet Cytogenet. 1994 Jul 15;75(2):156–157. doi: 10.1016/0165-4608(94)90171-6. [DOI] [PubMed] [Google Scholar]
  25. Speleman F., Dal Cin P., De Potter K., Laureys G., Roels H. J., Leroy J., Van Den Berghe H. Cytogenetic investigation of a case of congenital fibrosarcoma. Cancer Genet Cytogenet. 1989 May;39(1):21–24. doi: 10.1016/0165-4608(89)90224-0. [DOI] [PubMed] [Google Scholar]
  26. Sreekantaiah C., Karakousis C. P., Leong S. P., Sandberg A. A. Trisomy 8 as a nonrandom secondary change in myxoid liposarcoma. Cancer Genet Cytogenet. 1991 Feb;51(2):195–205. doi: 10.1016/0165-4608(91)90132-e. [DOI] [PubMed] [Google Scholar]
  27. Stephenson C. F., Berger C. S., Leong S. P., Davis J. R., Sandberg A. A. Ring chromosome in a dermatofibrosarcoma protuberans. Cancer Genet Cytogenet. 1992 Jan;58(1):52–54. doi: 10.1016/0165-4608(92)90133-s. [DOI] [PubMed] [Google Scholar]
  28. Suijkerbuijk R. F., Olde Weghuis D. E., Van den Berg M., Pedeutour F., Forus A., Myklebost O., Glier C., Turc-Carel C., Geurts van Kessel A. Comparative genomic hybridization as a tool to define two distinct chromosome 12-derived amplification units in well-differentiated liposarcomas. Genes Chromosomes Cancer. 1994 Apr;9(4):292–295. doi: 10.1002/gcc.2870090410. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES