Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1975 Apr;29(4):469–471. doi: 10.1128/am.29.4.469-471.1975

Effects of Bicarbonate on Growth of Neisseria gonorrhoeae: Replacement of Gaseous CO2 Atmosphere

Richard S Talley 1, Clarence L Baugh 1
PMCID: PMC187007  PMID: 804849

Abstract

The effect of NaHCO3 on the growth of Neisseria gonorrhoeae cultures was studied in a liquid and a semisolid growth medium. With a broth culture, NaHCO3 (0.009 M) greatly reduced the lag phase and also increased the total growth. The same concentration of bicarbonate supported rapid growth when added to the semisolid medium if the plates were individually incubated in sealed plastic bags. In a container with a large air space, a higher concentration of NaHCO3 was necessary to support growth. The assimilation of 14C-labeled NaHo3 by growing cultures was also investigated.

Full text

PDF
471

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. James-Holmquest A. N., Wende R. D., Mudd R. L., Williams R. P. Comparison of atmospheric conditions for culture of clinical specimens of Neisseria gonorrhoeae. Appl Microbiol. 1973 Oct;26(4):466–469. doi: 10.1128/am.26.4.466-469.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. La Scolea L. J., Jr, Young F. E. Development of a defined minimal medium for the growth of Neisseria gonorrhoeae. Appl Microbiol. 1974 Jul;28(1):70–76. doi: 10.1128/am.28.1.70-76.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Martin J. E., Armstrong J. H., Smith P. B. New system for cultivation of Neisseria gonorrhoeae. Appl Microbiol. 1974 Apr;27(4):802–805. doi: 10.1128/am.27.4.802-805.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. SURGALLA M. J., ANDREWS A. W., BAUGH C. L. EFFECTS OF BICARBONATE ON GROWTH OF PASTEURELLA PESTIS. I. DIFFERENTIAL RESPONSE OF VIRULENT AND AVIRULENT CELLS. J Bacteriol. 1964 Aug;88:269–272. doi: 10.1128/jb.88.2.269-272.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES