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. 1975 Nov;30(5):862–869. doi: 10.1128/am.30.5.862-869.1975

Effect of Some Inhibitors Derived from Nitrite on Macromolecular Synthesis in Bacillus cereus

J N Hansen 1, R A Levin 1
PMCID: PMC187285  PMID: 812424

Abstract

The effect of several inhibitors derived from nitrite on incorporation of [14C]uracil into ribonucleic acid of Bacillus cereus during outgrowth and vegetative growth has been determined. A heat-induced inhibitor presumably of the Perigo type was compared with the nitrosothiols of thioglycolate and β-mercaptoethanol. All were found to effectively inhibit uracil incorporation at all stages tested. Phase-contrast microscopy revealed that inhibition of morphological events occurred either before germination or during early outgrowth, depending on inhibitor concentration. It was also found that a precursor to the heat-induced inhibitor could be isolated from dialyzable tryptone (a pancreatic digest of casein), which chromatographed as a single species on diethylaminoethyl-cellulose and Sephadex G-25, with an apparent molecular weight of about 2,000.

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Selected References

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