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. 1988 Oct;133(1):5–12.

Expression of prostaglandin H synthase (cyclooxygenase) in Hodgkin's mononuclear and Reed-Sternberg cells. Functional resemblance between H-RS cells and histiocytes or interdigitating reticulum cells.

S M Hsu 1, P L Hsu 1, S S Lo 1, K K Wu 1
PMCID: PMC1880645  PMID: 3140669

Abstract

The synthesis of prostaglandin, prostacyclin, and thromboxane, which requires the enzyme prostaglandin H (PGH) synthase (cyclooxygenase), is a general property of histiocytes, monocytes, and Langerhans cells. Previously the authors reported the production of prostaglandin E2 in a Hodgkin's cell line, KM-H2, and suggested that these cells therefore have a functional similarity to histiocyte-related cells. The present study confirms that the Hodgkin's neoplastic (Reed-Sternberg, H-RS) cells in tissue are also capable of producing prostaglandins by demonstrating the presence of PGH synthase in these cells in B5-fixed, paraffin-embedded tissue sections. It was found that the H-RS cells from seven of ten patients with Hodgkin's disease were stained variously with anti-PGH synthase antibodies. In normal and reactive lymphoid tissues, anti-PGH synthase staining was restricted to histiocytes, endothelial cells, and interdigitating reticulum cells. Thus, this study provides further evidence for a possible relationship between H-RS cells and histiocytes or interdigitating reticulum cells; this relationship has also been supported by information obtained in extensive immunologic, biochemical, and cell-differentiation studies. The secretion of PGH-synthase products, especially prostaglandin E2, in H-RS cells may play a major role in the regulation of cellular immunity in patients with Hodgkin's disease.

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Selected References

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  1. Arenzana-Seisdedos F., Barbey S., Virelizier J. L., Kornprobst M., Nezelof C. Histiocytosis X. Purified (T6+) cells from bone granuloma produce interleukin 1 and prostaglandin E2 in culture. J Clin Invest. 1986 Jan;77(1):326–329. doi: 10.1172/JCI112296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bailey J. M., Bryant R. W., Low C. E., Pupillo M. B., Vanderhoek J. Y. Regulation of T-lymphocyte mitogenesis by the leukocyte product 15-hydroxy-eicosatetraenoic acid (15-HETE). Cell Immunol. 1982 Feb;67(1):112–120. doi: 10.1016/0008-8749(82)90203-9. [DOI] [PubMed] [Google Scholar]
  3. Browning J. L., Ribolini A. Interferon blocks interleukin 1-induced prostaglandin release from human peripheral monocytes. J Immunol. 1987 May 1;138(9):2857–2863. [PubMed] [Google Scholar]
  4. Burrichter H., Heit W., Schaadt M., Kirchner H., Diehl V. Production of colony-stimulating factors by Hodgkin cell lines. Int J Cancer. 1983 Mar 15;31(3):269–274. doi: 10.1002/ijc.2910310303. [DOI] [PubMed] [Google Scholar]
  5. Dayer J. M., de Rochemonteix B., Burrus B., Demczuk S., Dinarello C. A. Human recombinant interleukin 1 stimulates collagenase and prostaglandin E2 production by human synovial cells. J Clin Invest. 1986 Feb;77(2):645–648. doi: 10.1172/JCI112350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DeWitt D. L., Day J. S., Gauger J. A., Smith W. L. Monoclonal antibodies against PGH synthase: an immunoradiometric assay for quantitating the enzyme. Methods Enzymol. 1982;86:229–240. doi: 10.1016/0076-6879(82)86194-6. [DOI] [PubMed] [Google Scholar]
  7. Dinarello C. A., Marnoy S. O., Rosenwasser L. J. Role of arachidonate metabolism in the immunoregulatory function of human leukocytic pyrogen/lymphocyte-activating factor/interleukin 1. J Immunol. 1983 Feb;130(2):890–895. [PubMed] [Google Scholar]
  8. Drexler H. G., Gaedicke G., Lok M. S., Diehl V., Minowada J. Hodgkin's disease derived cell lines HDLM-2 and L-428: comparison of morphology, immunological and isoenzyme profiles. Leuk Res. 1986;10(5):487–500. doi: 10.1016/0145-2126(86)90084-6. [DOI] [PubMed] [Google Scholar]
  9. Farrar W. L., Humes J. L. The role of arachidonic acid metabolism in the activities of interleukin 1 and 2. J Immunol. 1985 Aug;135(2):1153–1159. [PubMed] [Google Scholar]
  10. Fisher R. I., Bates S. E., Bostick-Bruton F., Tuteja N., Diehl V. Neoplastic cells obtained from Hodgkin's disease function as accessory cells for mitogen-induced human T cell proliferative responses. J Immunol. 1984 May;132(5):2672–2677. [PubMed] [Google Scholar]
  11. Fisher R. I., Bostick-Bruton F., Sauder D. N., Scala G., Diehl V. Neoplastic cells obtained from Hodgkin's disease are potent stimulators of human primary mixed lymphocyte cultures. J Immunol. 1983 Jun;130(6):2666–2670. [PubMed] [Google Scholar]
  12. Fisher R. I. Implications of persistent T cell abnormalities for the etiology of Hodgkin's disease. Cancer Treat Rep. 1982 Apr;66(4):681–687. [PubMed] [Google Scholar]
  13. Hayari Y., Kukulansky T., Globerson A. Regulation of thymocyte proliferative response by macrophage-derived prostaglandin E2 and interleukin 1. Eur J Immunol. 1985 Jan;15(1):43–47. doi: 10.1002/eji.1830150109. [DOI] [PubMed] [Google Scholar]
  14. Hemler M., Lands W. E. Purification of the cyclooxygenase that forms prostaglandins. Demonstration of two forms of iron in the holoenzyme. J Biol Chem. 1976 Sep 25;251(18):5575–5579. [PubMed] [Google Scholar]
  15. Hillinger S. M., Herzig G. P. Impaired cell-mediated immunity in Hodgkin's disease mediated by suppressor lymphocytes and monocytes. J Clin Invest. 1978 Jun;61(6):1620–1627. doi: 10.1172/JCI109082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hsu S. M., Ho Y. S., Li P. J., Monheit J., Ree H. J., Sheibani K., Winberg C. D. L&H variants of Reed-Sternberg cells express sialylated Leu M1 antigen. Am J Pathol. 1986 Feb;122(2):199–203. [PMC free article] [PubMed] [Google Scholar]
  17. Hsu S. M., Hsu P. L. Phenotypes and phorbol ester-induced differentiation of human histiocytic lymphoma cell lines (U-937 and SU-DHL-1) and Reed-Sternberg cells. Am J Pathol. 1986 Feb;122(2):223–230. [PMC free article] [PubMed] [Google Scholar]
  18. Hsu S. M., Huang L. C., Hsu P. L., Ge Z. H., Ho Y. S., Cuttita F., Mulshine J. Biochemical and ultrastructural study of Leu M1 antigen in Reed-Sternberg cells: comparison with granulocytes and interdigitating reticulum cells. J Natl Cancer Inst. 1986 Aug;77(2):363–370. [PubMed] [Google Scholar]
  19. Hsu S. M., Jaffe E. S. Leu M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's disease. Am J Clin Pathol. 1984 Jul;82(1):29–32. doi: 10.1093/ajcp/82.1.29. [DOI] [PubMed] [Google Scholar]
  20. Hsu S. M., Pescovitz M. D., Hsu P. L. Monoclonal antibodies against SU-DHL-1 cells stain the neoplastic cells in true histiocytic lymphoma, malignant histiocytosis, and Hodgkin's disease. Blood. 1986 Jul;68(1):213–219. [PubMed] [Google Scholar]
  21. Hsu S. M. Phenotypic expression of cells of stationary elements in human lymphoid tissues. A histochemical and immunohistochemical study. Hematol Pathol. 1987;1(1):45–56. [PubMed] [Google Scholar]
  22. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  23. Hsu S. M., Soban E. Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. J Histochem Cytochem. 1982 Oct;30(10):1079–1082. doi: 10.1177/30.10.6182185. [DOI] [PubMed] [Google Scholar]
  24. Hsu S. M., Yang K., Jaffe E. S. Phenotypic expression of Hodgkin's and Reed-Sternberg cells in Hodgkin's disease. Am J Pathol. 1985 Feb;118(2):209–217. [PMC free article] [PubMed] [Google Scholar]
  25. Hsu S. M., Zhao X., Chakraborty S., Liu Y. F., Whang-Peng J., Lok M. S., Fukuhara S. Reed-Sternberg cells in Hodgkin's cell lines HDLM, L-428, and KM-H2 are not actively replicating: lack of bromodeoxyuridine uptake by multinuclear cells in culture. Blood. 1988 May;71(5):1382–1389. [PubMed] [Google Scholar]
  26. Hsu S. M., Zhao X. Expression of interleukin-1 in Reed-Sternberg cells and neoplastic cells from true histiocytic malignancies. Am J Pathol. 1986 Nov;125(2):221–225. [PMC free article] [PubMed] [Google Scholar]
  27. Hsu S. M., Zhao X., Hsu P. L., Lok M. S. Extracellular matrix does not induce the proliferation, but promotes the differentiation, of Hodgkin's cell line HDLM-1. Am J Pathol. 1987 Apr;127(1):9–14. [PMC free article] [PubMed] [Google Scholar]
  28. Hsu S. M., Zhao X. The H-RS-like cells in infectious mononucleosis are transformed interdigitating reticulum cells. Am J Pathol. 1987 May;127(2):403–408. [PMC free article] [PubMed] [Google Scholar]
  29. Kamesaki H., Fukuhara S., Tatsumi E., Uchino H., Yamabe H., Miwa H., Shirakawa S., Hatanaka M., Honjo T. Cytochemical, immunologic, chromosomal, and molecular genetic analysis of a novel cell line derived from Hodgkin's disease. Blood. 1986 Jul;68(1):285–292. [PubMed] [Google Scholar]
  30. Khansari N., Chou Y. K., Fudenberg H. H. Human monocyte heterogeneity: interleukin 1 and prostaglandin E2 production by separate subsets. Eur J Immunol. 1985 Jan;15(1):48–51. doi: 10.1002/eji.1830150110. [DOI] [PubMed] [Google Scholar]
  31. Knudsen P. J., Dinarello C. A., Strom T. B. Prostaglandins posttranscriptionally inhibit monocyte expression of interleukin 1 activity by increasing intracellular cyclic adenosine monophosphate. J Immunol. 1986 Nov 15;137(10):3189–3194. [PubMed] [Google Scholar]
  32. Kunkel S. L., Wiggins R. C., Chensue S. W., Larrick J. Regulation of macrophage tumor necrosis factor production by prostaglandin E2. Biochem Biophys Res Commun. 1986 May 29;137(1):404–410. doi: 10.1016/0006-291x(86)91224-6. [DOI] [PubMed] [Google Scholar]
  33. McGuire J. C., Richard K. A., Sun F. F., Tracey D. E. Production of prostaglandin D2 by murine macrophage cell lines. Prostaglandins. 1985 Dec;30(6):949–967. doi: 10.1016/0090-6980(85)90168-6. [DOI] [PubMed] [Google Scholar]
  34. Moore R. N., Pitruzzello F. J., Larsen H. S., Rouse B. T. Feedback regulation of colony-stimulating factor (CSF-1)-induced macrophage proliferation by endogenous E prostaglandins and interferon-alpha/beta. J Immunol. 1984 Aug;133(2):541–543. [PubMed] [Google Scholar]
  35. Newcom S. R., O'Rourke L. Potentiation of fibroblast growth by nodular sclerosing Hodgkin's disease cell cultures. Blood. 1982 Jul;60(1):228–237. [PubMed] [Google Scholar]
  36. Piacibello W., Rubin B. Y., Broxmeyer H. E. Prostaglandin E counteracts the gamma interferon induction of major histocompatibility complex class-II antigens on U937 cells and induction of responsiveness of U937 colony-forming cells to suppression by lactoferrin, transferrin, acidic isoferritins, and prostaglandin E. Exp Hematol. 1986 Jan;14(1):44–50. [PubMed] [Google Scholar]
  37. Rollins T. E., Smith W. L. Subcellular localization of prostaglandin-forming cyclooxygenase in Swiss mouse 3T3 fibroblasts by electron microscopic immunocytochemistry. J Biol Chem. 1980 May 25;255(10):4872–4875. [PubMed] [Google Scholar]
  38. Roth A., Kaufmann M. T., Cruchaud A., Dayer J. M. Human lymphocytes induce a differential release of prostaglandin E2 and interleukin 1-like mononuclear cell factor from normal blood monocytes. Eur J Immunol. 1985 Sep;15(9):960–963. doi: 10.1002/eji.1830150918. [DOI] [PubMed] [Google Scholar]
  39. Ruzicka T., Auböck J. Arachidonic acid metabolism in guinea pig Langerhans cells: studies on cyclooxygenase and lipoxygenase pathways. J Immunol. 1987 Jan 15;138(2):539–543. [PubMed] [Google Scholar]
  40. Salo R. J., Maddux N. L., Bleam D. K. Effect of prostaglandins on interferon synthesis in murine macrophage-like cell lines. Immunobiology. 1986 Mar;171(1-2):155–163. doi: 10.1016/S0171-2985(86)80024-9. [DOI] [PubMed] [Google Scholar]
  41. Smith W. L., Rollins T. E. Characteristics of rabbit anti-PGH synthase antibodies and use in immunocytochemistry. Methods Enzymol. 1982;86:213–222. doi: 10.1016/0076-6879(82)86192-2. [DOI] [PubMed] [Google Scholar]
  42. Spear G. T., Marshall P., Teodorescu M. Increase in proliferation and cytotoxic cell development in human mixed lymphocyte cultures in the presence of very low concentrations of LPS: role of IL-1 and prostaglandin E2. Clin Immunol Immunopathol. 1986 Jan;38(1):32–46. doi: 10.1016/0090-1229(86)90120-0. [DOI] [PubMed] [Google Scholar]
  43. Stobo J. D., Kennedy M. S., Goldyne M. E. Prostaglandin E modulation of the mitogenic response of human T cells. Differential response of T-cell subpopulations. J Clin Invest. 1979 Nov;64(5):1188–1203. doi: 10.1172/JCI109572. [DOI] [PMC free article] [PubMed] [Google Scholar]

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