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. 1994 May;38(5):969–972. doi: 10.1128/aac.38.5.969

Virucidal effect of myeloperoxidase on human immunodeficiency virus type 1-infected T cells.

J Chochola 1, Y Yamaguchi 1, N Moguilevsky 1, A Bollen 1, A D Strosberg 1, M Stanislawski 1
PMCID: PMC188135  PMID: 8067778

Abstract

Myeloperoxidase is virucidal to human immunodeficiency virus type 1 (HIV-1) in the persistently infected CEM human T-cell line or in acutely infected human peripheral blood mononuclear cells, as judged by viral infectivity and P24 radioimmunoassay. HIV-1 was specifically inactivated by low doses of the human myeloperoxidase (1.4 to 14.3 mU/ml) and the cells were spared. A higher enzyme concentration (143 mU/m) was cytotoxic, but uninfected CEM cells and normal lymphocytes were resistant to > or = 143 mU of myeloperoxidase per ml. The enzyme was virucidal with the Cl- present in medium and did not require exogenous H2O2. Catalase, an antioxidant enzyme, partially inhibited the virucidal effect of myeloperoxidase. Hence, the H2O2 probably came from the HIV-infected cells themselves. These in vitro findings indicate that the myeloperoxidase system is capable of inactivating HIV-1 of infected cells.

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Selected References

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