Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1992 Oct;141(4):881–886.

The expression of the Hu (paraneoplastic encephalomyelitis/sensory neuronopathy) antigen in human normal and tumor tissues.

J Dalmau 1, H M Furneaux 1, C Cordon-Cardo 1, J B Posner 1
PMCID: PMC1886624  PMID: 1415481

Abstract

Using immunohistochemistry or Western blot analysis, the authors have studied the expression of the Hu antigen (a neuronal protein identified by the serum of patients with small cell lung cancer and paraneoplastic encephalomyelitis/sensory neuronopathy) in normal human tissues and 115 tumors of different histologic types. In normal tissue, the Hu antigen is highly restricted to the nervous system. In lung tumors, the Hu antigen is restricted in its expression to all small cell carcinomas. A few other neuroendocrine-related tumors, especially neuroblastomas (50%), also express the antigen.

Full text

PDF
882

Images in this article

883Figure 1
on p.883
884Figure 2
on p.884
885Figure 3
on p.885

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson N. E., Rosenblum M. K., Graus F., Wiley R. G., Posner J. B. Autoantibodies in paraneoplastic syndromes associated with small-cell lung cancer. Neurology. 1988 Sep;38(9):1391–1398. doi: 10.1212/wnl.38.9.1391. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Budde-Steffen C., Anderson N. E., Rosenblum M. K., Posner J. B. Expression of an antigen in small cell lung carcinoma lines detected by antibodies from patients with paraneoplastic dorsal root ganglionpathy. Cancer Res. 1988 Jan 15;48(2):430–434. [PubMed] [Google Scholar]
  4. Cunningham J. M., Lennon V. A., Lambert E. H., Scheithauer B. Acetylcholine receptors in small cell carcinomas. J Neurochem. 1985 Jul;45(1):159–167. doi: 10.1111/j.1471-4159.1985.tb05488.x. [DOI] [PubMed] [Google Scholar]
  5. Dalmau J., Furneaux H. M., Gralla R. J., Kris M. G., Posner J. B. Detection of the anti-Hu antibody in the serum of patients with small cell lung cancer--a quantitative western blot analysis. Ann Neurol. 1990 May;27(5):544–552. doi: 10.1002/ana.410270515. [DOI] [PubMed] [Google Scholar]
  6. Dalmau J., Furneaux H. M., Rosenblum M. K., Graus F., Posner J. B. Detection of the anti-Hu antibody in specific regions of the nervous system and tumor from patients with paraneoplastic encephalomyelitis/sensory neuronopathy. Neurology. 1991 Nov;41(11):1757–1764. doi: 10.1212/wnl.41.11.1757. [DOI] [PubMed] [Google Scholar]
  7. Dalmau J., Graus F., Rosenblum M. K., Posner J. B. Anti-Hu--associated paraneoplastic encephalomyelitis/sensory neuronopathy. A clinical study of 71 patients. Medicine (Baltimore) 1992 Mar;71(2):59–72. doi: 10.1097/00005792-199203000-00001. [DOI] [PubMed] [Google Scholar]
  8. Djakiew D., Delsite R., Pflug B., Wrathall J., Lynch J. H., Onoda M. Regulation of growth by a nerve growth factor-like protein which modulates paracrine interactions between a neoplastic epithelial cell line and stromal cells of the human prostate. Cancer Res. 1991 Jun 15;51(12):3304–3310. [PubMed] [Google Scholar]
  9. Doyle A., Martin W. J., Funa K., Gazdar A., Carney D., Martin S. E., Linnoila I., Cuttitta F., Mulshine J., Bunn P. Markedly decreased expression of class I histocompatibility antigens, protein, and mRNA in human small-cell lung cancer. J Exp Med. 1985 May 1;161(5):1135–1151. doi: 10.1084/jem.161.5.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Furneaux H. F., Reich L., Posner J. B. Autoantibody synthesis in the central nervous system of patients with paraneoplastic syndromes. Neurology. 1990 Jul;40(7):1085–1091. doi: 10.1212/wnl.40.7.1085. [DOI] [PubMed] [Google Scholar]
  11. Furneaux H. M., Rosenblum M. K., Dalmau J., Wong E., Woodruff P., Graus F., Posner J. B. Selective expression of Purkinje-cell antigens in tumor tissue from patients with paraneoplastic cerebellar degeneration. N Engl J Med. 1990 Jun 28;322(26):1844–1851. doi: 10.1056/NEJM199006283222604. [DOI] [PubMed] [Google Scholar]
  12. Graus F., Cordon-Cardo C., Posner J. B. Neuronal antinuclear antibody in sensory neuronopathy from lung cancer. Neurology. 1985 Apr;35(4):538–543. doi: 10.1212/wnl.35.4.538. [DOI] [PubMed] [Google Scholar]
  13. Graus F., Elkon K. B., Cordon-Cardo C., Posner J. B. Sensory neuronopathy and small cell lung cancer. Antineuronal antibody that also reacts with the tumor. Am J Med. 1986 Jan;80(1):45–52. doi: 10.1016/0002-9343(86)90047-1. [DOI] [PubMed] [Google Scholar]
  14. Graus F., Ribalta T., Campo E., Monforte R., Urbano A., Rozman C. Immunohistochemical analysis of the immune reaction in the nervous system in paraneoplastic encephalomyelitis. Neurology. 1990 Feb;40(2):219–222. doi: 10.1212/wnl.40.2.219. [DOI] [PubMed] [Google Scholar]
  15. Lipinski M., Hirsch M. R., Deagostini-Bazin H., Yamada O., Tursz T., Goridis C. Characterization of neural cell adhesion molecules (NCAM) expressed by Ewing and neuroblastoma cell lines. Int J Cancer. 1987 Jul 15;40(1):81–86. doi: 10.1002/ijc.2910400115. [DOI] [PubMed] [Google Scholar]
  16. Posner J. B., Furneaux H. M. Paraneoplastic syndromes. Res Publ Assoc Res Nerv Ment Dis. 1990;68:187–219. [PubMed] [Google Scholar]
  17. Ruiter D. J., Mattijssen V., Broecker E. B., Ferrone S. MHC antigens in human melanomas. Semin Cancer Biol. 1991 Feb;2(1):35–45. [PubMed] [Google Scholar]
  18. Szabo A., Dalmau J., Manley G., Rosenfeld M., Wong E., Henson J., Posner J. B., Furneaux H. M. HuD, a paraneoplastic encephalomyelitis antigen, contains RNA-binding domains and is homologous to Elav and Sex-lethal. Cell. 1991 Oct 18;67(2):325–333. doi: 10.1016/0092-8674(91)90184-z. [DOI] [PubMed] [Google Scholar]
  19. Tanaka K., Isselbacher K. J., Khoury G., Jay G. Reversal of oncogenesis by the expression of a major histocompatibility complex class I gene. Science. 1985 Apr 5;228(4695):26–30. doi: 10.1126/science.3975631. [DOI] [PubMed] [Google Scholar]
  20. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES