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. 1992 Sep;141(3):571–578.

Characterization of glomerular epithelial cell matrix receptors.

S Adler 1
PMCID: PMC1886693  PMID: 1325740

Abstract

Integrin matrix receptors on glomerular epithelial cells (GEC) may play an important role in adhesion of GEC to the glomerular basement membrane (GBM) and in the maintenance of normal glomerular permeability. Therefore, the author determined the types of matrix receptors present on cultured rat GEC and examined their interactions with several components of the extracellular matrix. Beta 1 integrin matrix receptors were detected on all three glomerular cell types in rat kidney in vivo and at areas of cell-cell contact on cultured GEC. Glomerular epithelial cell adhesion to types I and IV collagen was slightly greater than to laminin and fibronectin. Adhesion to fibronectin was significantly inhibited by a synthetic peptide containing the RGD adhesion sequence. Immunoprecipitation of lysates of surface-iodinated GEC showed the presence of alpha 3 beta 1 integrin. Chromatography of lysates on immobilized collagen showed alpha 3 beta 1 integrin and a 70- to 75-kd protein band as the collagen receptors on GEC. Chromatography on the 120-kd cell-binding fragment of fibronectin disclosed only alpha 3 beta 1 as a specific fibronectin receptor. Antibody to the beta 1 integrin chain inhibited adhesion to laminin and collagen. These studies demonstrate that in vitro, as in vivo, GEC appear to express only alpha 3 beta 1 integrin. Furthermore, this matrix receptor is capable of mediating GEC adhesion to collagen, fibronectin, and laminin, components of the GBM, and presumably plays a similar role in promoting GEC adhesion to GBM in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrahamson D. R., Hein A., Caulfield J. P. Laminin in glomerular basement membranes of aminonucleoside nephrotic rats. Increased proteinuria induced by antilaminin immunoglobulin G. Lab Invest. 1983 Jul;49(1):38–47. [PubMed] [Google Scholar]
  2. Adler S., Baker P. J., Pritzl P., Couser W. G. Detection of terminal complement components in experimental immune glomerular injury. Kidney Int. 1984 Dec;26(6):830–837. doi: 10.1038/ki.1984.225. [DOI] [PubMed] [Google Scholar]
  3. Adler S. Inhibition of rat glomerular visceral epithelial cell growth by heparin. Am J Physiol. 1988 Oct;255(4 Pt 2):F781–F786. doi: 10.1152/ajprenal.1988.255.4.F781. [DOI] [PubMed] [Google Scholar]
  4. Akiyama S. K., Nagata K., Yamada K. M. Cell surface receptors for extracellular matrix components. Biochim Biophys Acta. 1990 Feb 28;1031(1):91–110. doi: 10.1016/0304-4157(90)90004-v. [DOI] [PubMed] [Google Scholar]
  5. Albelda S. M., Buck C. A. Integrins and other cell adhesion molecules. FASEB J. 1990 Aug;4(11):2868–2880. [PubMed] [Google Scholar]
  6. Carter W. G., Wayner E. A., Bouchard T. S., Kaur P. The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells. J Cell Biol. 1990 Apr;110(4):1387–1404. doi: 10.1083/jcb.110.4.1387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cosio F. G., Sedmak D. D., Nahman N. S., Jr Cellular receptors for matrix proteins in normal human kidney and human mesangial cells. Kidney Int. 1990 Nov;38(5):886–895. doi: 10.1038/ki.1990.287. [DOI] [PubMed] [Google Scholar]
  8. Courtoy P. J., Kanwar Y. S., Hynes R. O., Farquhar M. G. Fibronectin localization in the rat glomerulus. J Cell Biol. 1980 Dec;87(3 Pt 1):691–696. doi: 10.1083/jcb.87.3.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Courtoy P. J., Timpl R., Farquhar M. G. Comparative distribution of laminin, type IV collagen, and fibronectin in the rat glomerulus. J Histochem Cytochem. 1982 Sep;30(9):874–886. doi: 10.1177/30.9.7130672. [DOI] [PubMed] [Google Scholar]
  10. Elices M. J., Urry L. A., Hemler M. E. Receptor functions for the integrin VLA-3: fibronectin, collagen, and laminin binding are differentially influenced by Arg-Gly-Asp peptide and by divalent cations. J Cell Biol. 1991 Jan;112(1):169–181. doi: 10.1083/jcb.112.1.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gullberg D., Terracio L., Borg T. K., Rubin K. Identification of integrin-like matrix receptors with affinity for interstitial collagens. J Biol Chem. 1989 Jul 25;264(21):12686–12694. [PubMed] [Google Scholar]
  12. Harper P. A., Robinson J. M., Hoover R. L., Wright T. C., Karnovsky M. J. Improved methods for culturing rat glomerular cells. Kidney Int. 1984 Dec;26(6):875–880. doi: 10.1038/ki.1984.231. [DOI] [PubMed] [Google Scholar]
  13. Hynes R. O. Integrins: a family of cell surface receptors. Cell. 1987 Feb 27;48(4):549–554. doi: 10.1016/0092-8674(87)90233-9. [DOI] [PubMed] [Google Scholar]
  14. Hynes R. O., Marcantonio E. E., Stepp M. A., Urry L. A., Yee G. H. Integrin heterodimer and receptor complexity in avian and mammalian cells. J Cell Biol. 1989 Jul;109(1):409–420. doi: 10.1083/jcb.109.1.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kanwar Y. S., Rosenzweig L. J. Altered glomerular permeability as a result of focal detachment of the visceral epithelium. Kidney Int. 1982 Apr;21(4):565–574. doi: 10.1038/ki.1982.63. [DOI] [PubMed] [Google Scholar]
  16. Kaufmann R., Frösch D., Westphal C., Weber L., Klein C. E. Integrin VLA-3: ultrastructural localization at cell-cell contact sites of human cell cultures. J Cell Biol. 1989 Oct;109(4 Pt 1):1807–1815. doi: 10.1083/jcb.109.4.1807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kerjaschki D., Ojha P. P., Susani M., Horvat R., Binder S., Hovorka A., Hillemanns P., Pytela R. A beta 1-integrin receptor for fibronectin in human kidney glomeruli. Am J Pathol. 1989 Feb;134(2):481–489. [PMC free article] [PubMed] [Google Scholar]
  18. Korhonen M., Ylänne J., Laitinen L., Virtanen I. The alpha 1-alpha 6 subunits of integrins are characteristically expressed in distinct segments of developing and adult human nephron. J Cell Biol. 1990 Sep;111(3):1245–1254. doi: 10.1083/jcb.111.3.1245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Larjava H., Peltonen J., Akiyama S. K., Yamada S. S., Gralnick H. R., Uitto J., Yamada K. M. Novel function for beta 1 integrins in keratinocyte cell-cell interactions. J Cell Biol. 1990 Mar;110(3):803–815. doi: 10.1083/jcb.110.3.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Marcantonio E. E., Hynes R. O. Antibodies to the conserved cytoplasmic domain of the integrin beta 1 subunit react with proteins in vertebrates, invertebrates, and fungi. J Cell Biol. 1988 May;106(5):1765–1772. doi: 10.1083/jcb.106.5.1765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Martinez-Hernandez A., Chung A. E. The ultrastructural localization of two basement membrane components: entactin and laminin in rat tissues. J Histochem Cytochem. 1984 Mar;32(3):289–298. doi: 10.1177/32.3.6198358. [DOI] [PubMed] [Google Scholar]
  22. Mugnai G., Lewandowska K., Carnemolla B., Zardi L., Culp L. A. Modulation of matrix adhesive responses of human neuroblastoma cells by neighboring sequences in the fibronectins. J Cell Biol. 1988 Mar;106(3):931–943. doi: 10.1083/jcb.106.3.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ruoslahti E. Fibronectin and its receptors. Annu Rev Biochem. 1988;57:375–413. doi: 10.1146/annurev.bi.57.070188.002111. [DOI] [PubMed] [Google Scholar]
  24. Ruoslahti E., Pierschbacher M. D. New perspectives in cell adhesion: RGD and integrins. Science. 1987 Oct 23;238(4826):491–497. doi: 10.1126/science.2821619. [DOI] [PubMed] [Google Scholar]
  25. Simon E. E., McDonald J. A. Extracellular matrix receptors in the kidney cortex. Am J Physiol. 1990 Nov;259(5 Pt 2):F783–F792. doi: 10.1152/ajprenal.1990.259.5.F783. [DOI] [PubMed] [Google Scholar]
  26. Sugrue S. P. Isolation of collagen binding proteins from embryonic chicken corneal epithelial cells. J Biol Chem. 1987 Mar 5;262(7):3338–3343. [PubMed] [Google Scholar]
  27. Weening J. J., Van Guldener C., Daha M. R., Klar N., van der Wal A., Prins F. A. The pathophysiology of protein-overload proteinuria. Am J Pathol. 1987 Oct;129(1):64–73. [PMC free article] [PubMed] [Google Scholar]
  28. Whiteside C., Prutis K., Cameron R., Thompson J. Glomerular epithelial detachment, not reduced charge density, correlates with proteinuria in adriamycin and puromycin nephrosis. Lab Invest. 1989 Dec;61(6):650–660. [PubMed] [Google Scholar]

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