Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1993 Aug;143(2):350–354.

Cytotoxic potential of intraepithelial lymphocytes (IELs). Presence of TIA-1, the cytolytic granule-associated protein, in human IELs in normal and diseased intestine.

G J Russell 1, C Nagler-Anderson 1, P Anderson 1, A K Bhan 1
PMCID: PMC1887037  PMID: 8342592

Abstract

Human intestinal intraepithelial lymphocytes (IELs) have phenotypic characteristics of cytotoxic T cells, yet a cytotoxic function has not been demonstrated in redirected lysis assays. A monoclonal antibody that reacts with a cytotoxic granule-associated protein, TIA-1, was used in this study to identify this protein in many, but not all, IELs of normal human proximal small intestine. Furthermore, in active celiac disease, in which the number of IELs is significantly increased, a corresponding increase in the number of TIA-1 cells was found. These results indicate that whereas cytotoxicity of human IELs has been difficult to demonstrate, they contain at least one of the proteins associated with cytotoxicity, and a failure to demonstrate this function may be related to the in vitro assay system used.

Full text

PDF
354

Images in this article

353Figure 1
on p.353

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson P., Nagler-Anderson C., O'Brien C., Levine H., Watkins S., Slayter H. S., Blue M. L., Schlossman S. F. A monoclonal antibody reactive with a 15-kDa cytoplasmic granule-associated protein defines a subpopulation of CD8+ T lymphocytes. J Immunol. 1990 Jan 15;144(2):574–582. [PubMed] [Google Scholar]
  2. Cerf-Bensussan N., Guy-Grand D., Griscelli C. Intraepithelial lymphocytes of human gut: isolation, characterisation and study of natural killer activity. Gut. 1985 Jan;26(1):81–88. doi: 10.1136/gut.26.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cerf-Bensussan N., Schneeberger E. E., Bhan A. K. Immunohistologic and immunoelectron microscopic characterization of the mucosal lymphocytes of human small intestine by the use of monoclonal antibodies. J Immunol. 1983 Jun;130(6):2615–2622. [PubMed] [Google Scholar]
  4. Dobbins W. O., 3rd Human intestinal intraepithelial lymphocytes. Gut. 1986 Aug;27(8):972–985. doi: 10.1136/gut.27.8.972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ebert E. C., Roberts A. I., Brolin R. E., Raska K. Examination of the low proliferative capacity of human jejunal intraepithelial lymphocytes. Clin Exp Immunol. 1986 Jul;65(1):148–157. [PMC free article] [PubMed] [Google Scholar]
  6. Goodman T., Lefrancois L. Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J Exp Med. 1989 Nov 1;170(5):1569–1581. doi: 10.1084/jem.170.5.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Guy-Grand D., Malassis-Seris M., Briottet C., Vassalli P. Cytotoxic differentiation of mouse gut thymodependent and independent intraepithelial T lymphocytes is induced locally. Correlation between functional assays, presence of perforin and granzyme transcripts, and cytoplasmic granules. J Exp Med. 1991 Jun 1;173(6):1549–1552. doi: 10.1084/jem.173.6.1549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hameed A., Olsen K. J., Cheng L., Fox W. M., 3rd, Hruban R. H., Podack E. R. Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140(5):1025–1030. [PMC free article] [PubMed] [Google Scholar]
  9. Koizumi H., Liu C. C., Zheng L. M., Joag S. V., Bayne N. K., Holoshitz J., Young J. D. Expression of perforin and serine esterases by human gamma/delta T cells. J Exp Med. 1991 Feb 1;173(2):499–502. doi: 10.1084/jem.173.2.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lefrancois L., Goodman T. In vivo modulation of cytolytic activity and Thy-1 expression in TCR-gamma delta+ intraepithelial lymphocytes. Science. 1989 Mar 31;243(4899):1716–1718. doi: 10.1126/science.2564701. [DOI] [PubMed] [Google Scholar]
  11. Nagler-Anderson C., Lichtenheld M., Eisen H. N., Podack E. R. Perforin mRNA in primary peritoneal exudate cytotoxic T lymphocytes. J Immunol. 1989 Dec 1;143(11):3440–3443. [PubMed] [Google Scholar]
  12. Podack E. R., Hengartner H., Lichtenheld M. G. A central role of perforin in cytolysis? Annu Rev Immunol. 1991;9:129–157. doi: 10.1146/annurev.iy.09.040191.001021. [DOI] [PubMed] [Google Scholar]
  13. Russell G. J., Winter H. S., Fox V. L., Bhan A. K. Lymphocytes bearing the gamma delta T-cell receptor in normal human intestine and celiac disease. Hum Pathol. 1991 Jul;22(7):690–694. doi: 10.1016/0046-8177(91)90291-v. [DOI] [PubMed] [Google Scholar]
  14. Sale G. E., Anderson P., Browne M., Myerson D. Evidence of cytotoxic T-cell destruction of epidermal cells in human graft-vs-host disease. Immunohistology with monoclonal antibody TIA-1. Arch Pathol Lab Med. 1992 Jun;116(6):622–625. [PubMed] [Google Scholar]
  15. Selby W. S., Janossy G., Goldstein G., Jewell D. P. T lymphocyte subsets in human intestinal mucosa: the distribution and relationship to MHC-derived antigens. Clin Exp Immunol. 1981 Jun;44(3):453–458. [PMC free article] [PubMed] [Google Scholar]
  16. Selby W. S., Janossy G., Jewell D. P. Immunohistological characterisation of intraepithelial lymphocytes of the human gastrointestinal tract. Gut. 1981 Mar;22(3):169–176. doi: 10.1136/gut.22.3.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Taunk J., Roberts A. I., Ebert E. C. Spontaneous cytotoxicity of human intraepithelial lymphocytes against epithelial cell tumors. Gastroenterology. 1992 Jan;102(1):69–75. doi: 10.1016/0016-5085(92)91785-3. [DOI] [PubMed] [Google Scholar]
  18. Tian Q., Streuli M., Saito H., Schlossman S. F., Anderson P. A polyadenylate binding protein localized to the granules of cytolytic lymphocytes induces DNA fragmentation in target cells. Cell. 1991 Nov 1;67(3):629–639. doi: 10.1016/0092-8674(91)90536-8. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES