Abstract
Hepatocyte paraffin 1 is a monoclonal antibody that has been developed specifically to react with hepatocytes in routine formalin-fixed and paraffin-embedded surgical pathology tissues. It results in a distinct, granular cytoplasmic staining of hepatocytes but fails to react with bile ducts and nonparenchymal liver cells. The antibody decorates a majority of hepatocellular carcinomas, including fibrolamellar variants. It fails to react with a wide variety of other adult malignancies, with the exception of focal staining in a few gastrointestinal malignancies, including a subpopulation of gastric carcinomas.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bradley L. A., Franco E. L., Reisner H. M. Use of monoclonal antibodies in an enzyme immunoassay for factor VIII-related antigen. Clin Chem. 1984 Jan;30(1):87–92. [PubMed] [Google Scholar]
- Johnson D. E., Powers C. N., Rupp G., Frable W. J. Immunocytochemical staining of fine-needle aspiration biopsies of the liver as a diagnostic tool for hepatocellular carcinoma. Mod Pathol. 1992 Mar;5(2):117–123. [PubMed] [Google Scholar]
- Kearney J. F., Radbruch A., Liesegang B., Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979 Oct;123(4):1548–1550. [PubMed] [Google Scholar]
- Koelma I. A., Nap M., Huitema S., Krom R. A., Houthoff H. J. Hepatocellular carcinoma, adenoma, and focal nodular hyperplasia. Comparative histopathologic study with immunohistochemical parameters. Arch Pathol Lab Med. 1986 Nov;110(11):1035–1040. [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Lubahn B. C., Reisner H. M. Characterization of a monoclonal anti-idiotype antibody to human anti-factor VIII antibodies. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8232–8236. doi: 10.1073/pnas.87.21.8232. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miettinen M. Immunohistochemistry of solid tumors. Brief review of selected problems. APMIS. 1990 Mar;98(3):191–199. doi: 10.1111/j.1699-0463.1990.tb01021.x. [DOI] [PubMed] [Google Scholar]
- Taketa K. Alpha-fetoprotein: reevaluation in hepatology. Hepatology. 1990 Dec;12(6):1420–1432. doi: 10.1002/hep.1840120625. [DOI] [PubMed] [Google Scholar]