Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1994 Jan;144(1):171–176.

Effect of interleukin-4 on the synthesis of the third component of complement by pulmonary epithelial cells.

K K Christian-Ritter 1, L D Hill 1, E B Hoie 1, T L Zach 1
PMCID: PMC1887117  PMID: 8291606

Abstract

Complement activation in the lung is important in a variety of physiological and pathological conditions. The third component of complement, C3, is the pivotal constituent of the complement cascade. C3 is produced in the lung by several cell types including pulmonary epithelial cells. Because pulmonary epithelial cells and T lymphocytes may interact within the lung to regulate local immune responses, we examined the effect of a T lymphocyte-derived cytokine, interleukin-4 (IL-4) on C3 production by A549 human pulmonary epithelial cells. Treatment of A549 cells with IL-4 increased C3 production in a time- and dose-dependent fashion. Concentrations of IL-4 > or = 0.1 ng/ml significantly increased C3 production. Maximal increase in C3 synthesis occurred after stimulation of A549 cells with IL-4 (10 ng/ml) for 3 days. Preincubation of IL-4 with a neutralizing anti-human IL-4 antibody inhibited IL-4's effect on C3 production. The relative abundance of C3 messenger RNA levels in A549 cells increased following IL-4 treatment, indicating that IL-4's effects on C3 production were pretranslational. Intercellular communication between T lymphocytes and pulmonary epithelial cells via cytokines such as IL-4 may be important in the local regulation of C3 gene expression during the inflammatory response.

Full text

PDF
174

Images in this article

174Figure 4
on p.174

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Badley J. E., Bishop G. A., St John T., Frelinger J. A. A simple, rapid method for the purification of poly A+ RNA. Biotechniques. 1988 Feb;6(2):114–116. [PubMed] [Google Scholar]
  2. Cole F. S., Matthews W. J., Jr, Rossing T. H., Gash D. J., Lichtenberg N. A., Pennington J. E. Complement biosynthesis by human bronchoalveolar macrophages. Clin Immunol Immunopathol. 1983 May;27(2):153–159. doi: 10.1016/0090-1229(83)90065-x. [DOI] [PubMed] [Google Scholar]
  3. Hart P. H., Burgess D. R., Vitti G. F., Hamilton J. A. Interleukin-4 stimulates human monocytes to produce tissue-type plasminogen activator. Blood. 1989 Sep;74(4):1222–1225. [PubMed] [Google Scholar]
  4. Howard M., Farrar J., Hilfiker M., Johnson B., Takatsu K., Hamaoka T., Paul W. E. Identification of a T cell-derived b cell growth factor distinct from interleukin 2. J Exp Med. 1982 Mar 1;155(3):914–923. doi: 10.1084/jem.155.3.914. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Katz Y., Strunk R. C. Enhanced synthesis of factor B induced by tumor necrosis factor in human skin fibroblasts is decreased by IL-4. J Immunol. 1990 Jun 15;144(12):4675–4680. [PubMed] [Google Scholar]
  6. Littman B. H., Dastvan F. F., Carlson P. L., Sanders K. M. Regulation of monocyte/macrophage C2 production and HLA-DR expression by IL-4 (BSF-1) and IFN-gamma. J Immunol. 1989 Jan 15;142(2):520–525. [PubMed] [Google Scholar]
  7. Phillips J. O., Everson M. P., Moldoveanu Z., Lue C., Mestecky J. Synergistic effect of IL-4 and IFN-gamma on the expression of polymeric Ig receptor (secretory component) and IgA binding by human epithelial cells. J Immunol. 1990 Sep 15;145(6):1740–1744. [PubMed] [Google Scholar]
  8. Rabin E. M., Ohara J., Paul W. E. B-cell stimulatory factor 1 activates resting B cells. Proc Natl Acad Sci U S A. 1985 May;82(9):2935–2939. doi: 10.1073/pnas.82.9.2935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rothman B. L., Despins A. W., Kreutzer D. L. Cytokine regulation of C3 and C5 production by the human type II pneumocyte cell line, A549. J Immunol. 1990 Jul 15;145(2):592–598. [PubMed] [Google Scholar]
  10. Rothman B. L., Merrow M., Bamba M., Kennedy T., Kreutzer D. L. Biosynthesis of the third and fifth complement components by isolated human lung cells. Am Rev Respir Dis. 1989 Jan;139(1):212–220. doi: 10.1164/ajrccm/139.1.212. [DOI] [PubMed] [Google Scholar]
  11. Rothman B. L., Merrow M., Despins A., Kennedy T., Kreutzer D. L. Effect of lipopolysaccharide on C3 and C5 production by human lung cells. J Immunol. 1989 Jul 1;143(1):196–202. [PubMed] [Google Scholar]
  12. Sato T., Hong M. H., Jin C. H., Ishimi Y., Udagawa N., Shinki T., Abe E., Suda T. The specific production of the third component of complement by osteoblastic cells treated with 1 alpha,25-dihydroxyvitamin D3. FEBS Lett. 1991 Jul 8;285(1):21–24. doi: 10.1016/0014-5793(91)80715-f. [DOI] [PubMed] [Google Scholar]
  13. Spits H., Yssel H., Takebe Y., Arai N., Yokota T., Lee F., Arai K., Banchereau J., de Vries J. E. Recombinant interleukin 4 promotes the growth of human T cells. J Immunol. 1987 Aug 15;139(4):1142–1147. [PubMed] [Google Scholar]
  14. Standiford T. J., Strieter R. M., Kasahara K., Kunkel S. L. Disparate regulation of interleukin 8 gene expression from blood monocytes, endothelial cells, and fibroblasts by interleukin 4. Biochem Biophys Res Commun. 1990 Sep 14;171(2):531–536. doi: 10.1016/0006-291x(90)91178-u. [DOI] [PubMed] [Google Scholar]
  15. Strunk R. C., Eidlen D. M., Mason R. J. Pulmonary alveolar type II epithelial cells synthesize and secrete proteins of the classical and alternative complement pathways. J Clin Invest. 1988 May;81(5):1419–1426. doi: 10.1172/JCI113472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sundstrom S. A., Komm B. S., Ponce-de-Leon H., Yi Z., Teuscher C., Lyttle C. R. Estrogen regulation of tissue-specific expression of complement C3. J Biol Chem. 1989 Oct 5;264(28):16941–16947. [PubMed] [Google Scholar]
  17. Thornhill M. H., Haskard D. O. IL-4 regulates endothelial cell activation by IL-1, tumor necrosis factor, or IFN-gamma. J Immunol. 1990 Aug 1;145(3):865–872. [PubMed] [Google Scholar]
  18. Wieser M., Bonifer R., Oster W., Lindemann A., Mertelsmann R., Herrmann F. Interleukin-4 induces secretion of CSF for granulocytes and CSF for macrophages by peripheral blood monocytes. Blood. 1989 Apr;73(5):1105–1108. [PubMed] [Google Scholar]
  19. Zach T. L., Hill L. D., Herrman V. A., Leuschen M. P., Hostetter M. K. Effect of glucocorticoids on C3 gene expression by the A549 human pulmonary epithelial cell line. J Immunol. 1992 Jun 15;148(12):3964–3969. [PubMed] [Google Scholar]
  20. de Bruijn M. H., Fey G. H. Human complement component C3: cDNA coding sequence and derived primary structure. Proc Natl Acad Sci U S A. 1985 Feb;82(3):708–712. doi: 10.1073/pnas.82.3.708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. te Velde A. A., Huijbens R. J., Heije K., de Vries J. E., Figdor C. G. Interleukin-4 (IL-4) inhibits secretion of IL-1 beta, tumor necrosis factor alpha, and IL-6 by human monocytes. Blood. 1990 Oct 1;76(7):1392–1397. [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES