Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1986 May;123(2):301–309.

Immunohistochemical analysis of monoclonal antibodies to renal antigens. Application in the diagnosis of renal cell carcinoma.

E Oosterwijk, D J Ruiter, J C Wakka, J W Huiskens-van der Meij, U Jonas, G J Fleuren, J Zwartendijk, P Hoedemaeker, S O Warnaar
PMCID: PMC1888313  PMID: 3706491

Abstract

Four mouse monoclonal antibodies reacting with renal proximal epithelium were characterized on normal adult tissues, fetal kidneys, and various malignancies. These four antibodies were found to react with renal cell carcinomas (RCCs) but not with a variety of other tumors. Three antibodies react only with some primary RCCs and not with metastatic RCCs. The fourth antibody, RC 38, reacts with 46 of 47 primary RCCs tested and with 8 of 13 RCC metastases. No reaction was seen with 179 tumors of various origin, which indicates that this antibody is very useful in diagnosing RCC.

Full text

PDF
305

Images in this article

305Figure 1
on p.305
306Figure 2
on p.306

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bander N. H. Comparison of antigen expression of human renal cancers in vivo and in vitro. Cancer. 1984 Mar 15;53(6):1235–1239. doi: 10.1002/1097-0142(19840315)53:6<1235::aid-cncr2820530603>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  2. Colcher D., Hand P. H., Nuti M., Schlom J. A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc Natl Acad Sci U S A. 1981 May;78(5):3199–3203. doi: 10.1073/pnas.78.5.3199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cordon-Cardo C., Bander N. H., Fradet Y., Finstad C. L., Whitmore W. F., Lloyd K. O., Oettgen H. F., Melamed M. R., Old L. J. Immunoanatomic dissection of the human urinary tract by monoclonal antibodies. J Histochem Cytochem. 1984 Oct;32(10):1035–1040. doi: 10.1177/32.10.6384360. [DOI] [PubMed] [Google Scholar]
  4. Finstad C. L., Cordon-Cardo C., Bander N. H., Whitmore W. F., Melamed M. R., Old L. J. Specificity analysis of mouse monoclonal antibodies defining cell surface antigens of human renal cancer. Proc Natl Acad Sci U S A. 1985 May;82(9):2955–2959. doi: 10.1073/pnas.82.9.2955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hancock W. W., Atkins R. C. Monoclonal antibodies to human glomerular cells: a marker for glomerular epithelial cells. Nephron. 1983;33(2):83–90. doi: 10.1159/000182918. [DOI] [PubMed] [Google Scholar]
  6. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  7. Magnani J. L., Steplewski Z., Koprowski H., Ginsburg V. Identification of the gastrointestinal and pancreatic cancer-associated antigen detected by monoclonal antibody 19-9 in the sera of patients as a mucin. Cancer Res. 1983 Nov;43(11):5489–5492. [PubMed] [Google Scholar]
  8. Moon T. D., Vessella R. L., Palme D. F., Nowak J. A., Lange P. H. A highly restricted antigen for renal cell carcinoma defined by a monoclonal antibody. Hybridoma. 1985 Summer;4(2):163–171. doi: 10.1089/hyb.1985.4.163. [DOI] [PubMed] [Google Scholar]
  9. Müller G. A., Müller C. Characterisation of renal antigens on distinct parts of the human nephron by monoclonal antibodies. Klin Wochenschr. 1983 Sep 15;61(18):893–902. doi: 10.1007/BF01537529. [DOI] [PubMed] [Google Scholar]
  10. OBERLING C., RIVIERE M., HAGUENAU F. Ultrastructure of the clear cells in renal carcinomas and its importance for the demonstration of their renal origin. Nature. 1960 Apr 30;186:402–403. doi: 10.1038/186402a0. [DOI] [PubMed] [Google Scholar]
  11. Schenk E. A., Schwartz R. H., Lewis R. A. Tamm-Horsfall mucoprotein. I. Localization in the kidney. Lab Invest. 1971 Jul;25(1):92–95. [PubMed] [Google Scholar]
  12. Sharon J., Morrison S. L., Kabat E. A. Detection of specific hybridoma clones by replica immunoadsorption of their secreted antibodies. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1420–1424. doi: 10.1073/pnas.76.3.1420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ueda R., Ogata S., Morrissey D. M., Finstad C. L., Szkudlarek J., Whitmore W. F., Oettgen H. F., Lloyd K. O., Old L. J. Cell surface antigens of human renal cancer defined by mouse monoclonal antibodies: identification of tissue-specific kidney glycoproteins. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5122–5126. doi: 10.1073/pnas.78.8.5122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Vennegoor C., Calafat J., Hageman P., van Buitenen F., Janssen H., Kolk A., Rümke P. Biochemical characterization and cellular localization of a formalin-resistant melanoma-associated antigen reacting with monoclonal antibody NKI/C-3. Int J Cancer. 1985 Mar 15;35(3):287–295. doi: 10.1002/ijc.2910350302. [DOI] [PubMed] [Google Scholar]
  15. Wallace A. C., Nairn R. C. Renal tubular antigens in kidney tumors. Cancer. 1972 Apr;29(4):977–981. doi: 10.1002/1097-0142(197204)29:4<977::aid-cncr2820290444>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
  16. Warnke R. A., Gatter K. C., Falini B., Hildreth P., Woolston R. E., Pulford K., Cordell J. L., Cohen B., De Wolf-Peeters C., Mason D. Y. Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med. 1983 Nov 24;309(21):1275–1281. doi: 10.1056/NEJM198311243092102. [DOI] [PubMed] [Google Scholar]
  17. Wilson B. S., Imai K., Natali P. G., Ferrone S. Distribution and molecular characterization of a cell-surface and a cytoplasmic antigen detectable in human melanoma cells with monoclonal antibodies. Int J Cancer. 1981 Sep 15;28(3):293–300. doi: 10.1002/ijc.2910280307. [DOI] [PubMed] [Google Scholar]
  18. Wright G. L., Jr, Beckett M. L., Starling J. J., Schellhammer P. F., Sieg S. M., Ladaga L. E., Poleskic S. Immunohistochemical localization of prostate carcinoma-associated antigens. Cancer Res. 1983 Nov;43(11):5509–5516. [PubMed] [Google Scholar]
  19. van Muijen G. N., Ruiter D. J., Ponec M., Huiskens-van der, Mey C., Warnaar S. O. Monoclonal antibodies with different specificities against cytokeratins. An immunohistochemical study of normal tissues and tumors. Am J Pathol. 1984 Jan;114(1):9–17. [PMC free article] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES