Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1986 Sep;124(3):496–509.

An adenoid squamous carcinoma-forming cell line established from an oral keratinizing squamous cell carcinoma expressing carcinoembryonic antigen.

T Yanagawa, Y Hayashi, H Yoshida, Y Yura, S Nagamine, T Bando, M Sato
PMCID: PMC1888346  PMID: 2429552

Abstract

A neoplastic epithelial cell line, TYS, was isolated from a well-differentiated squamous cell carcinoma expressing carcinoembryonic antigen (CEA) that arose in human oral mucosa. Expressions of CEA and amylase as well as ample tonofilaments were detected in cultured TYS cells. Transplantation of the cells into athymic nude mice resulted in production of adenoid squamous cell carcinoma containing CEA and amylase. Cultivation of TYS cells in the presence of sodium butyrate resulted in suppression of cell growth and production of secretory granules with amylase in the cytoplasm of the cells. When the sodium butyrate-treated cells were transplanted into nude mice, a small mass developed transiently at the inoculation site and then disappeared. This mass was histopathologically interpreted as acinic cell carcinoma with squamoid lesion. These findings suggest that we have established a human adenoid squamous carcinoma cell line presumably derived from a minor salivary gland present in oral mucosa.

Full text

PDF
497

Images in this article

500Figure 1
on p.500
501Figure 2
on p.501
502Figure 3
on p.502
503Figure 4
on p.503
505Figure 6
on p.505
506Figure 7
on p.506
507Figure 8
on p.507

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHERRY C. P., GLUCKSMANN A. Incidence, histology, and response to radiation of mixed carcinomas (adenoacanthomas) of the uterine cervix. Cancer. 1956 Sep-Oct;9(5):971–979. doi: 10.1002/1097-0142(195609/10)9:5<971::aid-cncr2820090518>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  2. Ceska M., Birath K., Brown B. A new and rapid method for the clinical determination of alpha-amylase activities in human serum and urine. Optimal conditions. Clin Chim Acta. 1969 Dec;26(3):437–444. doi: 10.1016/0009-8981(69)90071-0. [DOI] [PubMed] [Google Scholar]
  3. DOUGHERTY C. M., COTTEN N. MIXED SQUAMOUS-CELL AND ADENOCARCINOMA OF THE CERVIX. COMBINED, ADENOSQUAMOUS, AND MUCOEPIDERMOID TYPES. Cancer. 1964 Sep;17:1132–1143. doi: 10.1002/1097-0142(196409)17:9<1132::aid-cncr2820170906>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
  4. Egan M. L., Todd C. W. Carcinoembryonic antigen: synthesis by a continuous line of adenocarcinoma cells. J Natl Cancer Inst. 1972 Sep;49(3):887–889. [PubMed] [Google Scholar]
  5. FRIEDMAN M., HALL J. W. Radiation-induced squamous-cell metaplasia and hyperplasia of the normal mucous glands of the oral cavity. Radiology. 1950 Dec;55(6):848–851. doi: 10.1148/55.6.848. [DOI] [PubMed] [Google Scholar]
  6. Gerughty R. M., Hennigar G. R., Brown F. M. Adenosquamous carcinoma of the nasal, oral and laryngeal cavities. A clinicopathologic survey of ten cases. Cancer. 1968 Dec;22(6):1140–1155. doi: 10.1002/1097-0142(196811)22:6<1140::aid-cncr2820220610>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  7. Goldenberg D. M., Sharkey R. M., Primus F. J. Immunocytochemical detection of carcinoembryonic antigen in conventional histopathology specimens. Cancer. 1978 Sep;42(3 Suppl):1546–1553. doi: 10.1002/1097-0142(197809)42:3+<1546::aid-cncr2820420829>3.0.co;2-d. [DOI] [PubMed] [Google Scholar]
  8. Jacoway J. R., Nelson J. F., Boyers R. C. Adenoid squamous-cell carcinoma (adenocanthoma) of the oral labial mucosa. A clinicopathologic study of fifteen cases. Oral Surg Oral Med Oral Pathol. 1971 Sep;32(3):444–449. doi: 10.1016/0030-4220(71)90205-2. [DOI] [PubMed] [Google Scholar]
  9. Johnson W. C., Helwig E. B. Adenoid squamous cell carcinoma (adenoacanthoma). A clinicopathologic study of 155 patients. Cancer. 1966 Nov;19(11):1639–1650. doi: 10.1002/1097-0142(196611)19:11<1639::aid-cncr2820191131>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  10. Lasser A., Cornog J. L., Morris J. M. Adenoid squamous cell carcinoma of the vulva. Cancer. 1974 Jan;33(1):224–227. doi: 10.1002/1097-0142(197401)33:1<224::aid-cncr2820330133>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  11. MACPHERSON I., MONTAGNIER L. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS. Virology. 1964 Jun;23:291–294. doi: 10.1016/0042-6822(64)90301-0. [DOI] [PubMed] [Google Scholar]
  12. MULLER S. A., WILHELMJ C. M., Jr, HARRISON E. G., Jr, WINKELMANN R. K. ADENOID SQUAMOUS CELL CARCINOMA (ADENOACANTHOMA OF LEVER). REPORT OF SEVEN CASES AND REVIEW. Arch Dermatol. 1964 Apr;89:589–597. doi: 10.1001/archderm.1964.01590280089016. [DOI] [PubMed] [Google Scholar]
  13. McLean I. W., Nakane P. K. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem. 1974 Dec;22(12):1077–1083. doi: 10.1177/22.12.1077. [DOI] [PubMed] [Google Scholar]
  14. OLANSKY S., SMITH J. G., Jr, WANSKER B. A. Adenoacanthoma; a dyskeratotic squamous-cell carcinoma with tubular and alveolar formations. AMA Arch Derm. 1957 Jan;75(1):96–100. [PubMed] [Google Scholar]
  15. Prasad K. N., Sinha P. K. Effect of sodium butyrate on mammalian cells in culture: a review. In Vitro. 1976 Feb;12(2):125–132. doi: 10.1007/BF02796360. [DOI] [PubMed] [Google Scholar]
  16. Sternberger L. A., Hardy P. H., Jr, Cuculis J. J., Meyer H. G. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem. 1970 May;18(5):315–333. doi: 10.1177/18.5.315. [DOI] [PubMed] [Google Scholar]
  17. Takagi M., Sakota Y., Takayama S., Ishikawa G. Adenoid squamous cell carcinoma of the oral mucosa: report of two autopsy cases. Cancer. 1977 Nov;40(5):2250–2255. doi: 10.1002/1097-0142(197711)40:5<2250::aid-cncr2820400535>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  18. Tomich C. E., Hutton C. E. Adenoid squamous cell carcinoma of the lip: report of cases. J Oral Surg. 1972 Aug;30(8):592–598. [PubMed] [Google Scholar]
  19. Tsao D., Shi Z. R., Wong A., Kim Y. S. Effect of sodium butyrate on carcinoembryonic antigen production by human colonic adenocarcinoma cells in culture. Cancer Res. 1983 Mar;43(3):1217–1222. [PubMed] [Google Scholar]
  20. Weitzner S. Adenoid squamous-cell carcinoma of vermilion mucosa of lower lip. Oral Surg Oral Med Oral Pathol. 1974 Apr;37(4):589–593. doi: 10.1016/0030-4220(74)90291-6. [DOI] [PubMed] [Google Scholar]
  21. van Nagell J. R., Jr, Hudson S., Gay E. C., Donaldson E. S., Hanson M., Powell D. F., Goldenberg D. M. Carcinoembryonic antigen in carcinoma of the uterine cervix: antigen distribution in primary and metastatic tumors. Cancer. 1982 Jan 15;49(2):379–383. doi: 10.1002/1097-0142(19820115)49:2<379::aid-cncr2820490228>3.0.co;2-r. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES