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. 1987 Apr;127(1):1–8.

Monoclonal antibodies marking T lymphocytes in paraffin-embedded tissue.

J Linder, Y L Ye, D S Harrington, J O Armitage, D D Weisenburger
PMCID: PMC1899597  PMID: 3494405

Abstract

The authors investigated the ability of 70 monoclonal antibodies obtained from the Third International Workshop on Human Leukocyte Antigens (Oxford, 1986) to mark T lymphocytes in B5-fixed paraffin-embedded tissue. No staining occurred with 65 of the antibodies; however, 5 antibodies marked small lymphocytes in the T-cell areas of human tonsil. Two antibodies which strongly labeled lymphocytes, UCHL1 and T2/48, were used to examine 106 cases of non-Hodgkin's lymphoma, 29 cases of Hodgkin's disease, and a variety of normal and neoplastic tissues. UCHL1 and T2/48 each marked 86% (37/43) of B5-fixed T-cell lymphomas. Only 50% of formalin-fixed T-cell lymphomas were marked with these antibodies. UCHL1 marked 1.8% (1/56) of the B-cell lymphomas, compared with T2/48, which marked 19.6% (11/56) of the B-cell lymphomas. T2/48 had the interesting attribute of marking cells of the follicular mantle-zone and intermediate lymphocytic lymphoma, suggesting that the antibody recognizes a B-cell differentiation antigen. No Reed-Sternberg cells, epithelial neoplasms, sarcomas, neurogenic tumors, or normal nonlymphoid tissue were marked by either antibody. These antibodies successfully mark T cells in paraffin tissue sections and should aid in the investigation and characterization of abnormal lymphoid proliferations, "undifferentiated" malignant neoplasms, and immunologically mediated disorders.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Battifora H., Trowbridge I. S. A monoclonal antibody useful for the differential diagnosis between malignant lymphoma and nonhematopoietic neoplasms. Cancer. 1983 Mar 1;51(5):816–821. doi: 10.1002/1097-0142(19830301)51:5<816::aid-cncr2820510512>3.0.co;2-4. [DOI] [PubMed] [Google Scholar]
  2. Berg A. R., Linder J., Anderson R. W., Tempero M. A., Edney J. A., Armitage J. O. The undifferentiated malignant neoplasm. Identification of lymphoma arising in skeletal muscle by immunohistochemical analysis. JAMA. 1985 Nov 8;254(18):2625–2626. doi: 10.1001/jama.254.18.2625. [DOI] [PubMed] [Google Scholar]
  3. Bloomfield C. D., Kersey J. H., Brunning R. D., Gajl-Peczalska K. J. Prognostic significance of lymphocyte surface markers in adult non-Hodgkin's malignant lymphoma. Lancet. 1976 Dec 18;2(7999):1330–1333. doi: 10.1016/s0140-6736(76)91976-0. [DOI] [PubMed] [Google Scholar]
  4. Clark K. A., Keren D. F. Demonstration of monoclonal lymphoplasmacytic proliferations by immunofluorescence on routine formalin-fixed, paraffin-embedded tissue. Cancer. 1982 Jun 1;49(11):2376–2382. doi: 10.1002/1097-0142(19820601)49:11<2376::aid-cncr2820491126>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  5. Epstein A. L., Marder R. J., Winter J. N., Fox R. I. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984 Aug;133(2):1028–1036. [PubMed] [Google Scholar]
  6. Falini B., Taylor C. R. New developments in immunoperoxidase techniques and their application. Arch Pathol Lab Med. 1983 Mar;107(3):105–117. [PubMed] [Google Scholar]
  7. Foon K. A., Schroff R. W., Gale R. P. Surface markers on leukemia and lymphoma cells: recent advances. Blood. 1982 Jul;60(1):1–19. [PubMed] [Google Scholar]
  8. Kurtin P. J., Pinkus G. S. Leukocyte common antigen--a diagnostic discriminant between hematopoietic and nonhematopoietic neoplasms in paraffin sections using monoclonal antibodies: correlation with immunologic studies and ultrastructural localization. Hum Pathol. 1985 Apr;16(4):353–365. doi: 10.1016/s0046-8177(85)80229-x. [DOI] [PubMed] [Google Scholar]
  9. Marder R. J., Variakojis D., Silver J., Epstein A. L. Immunohistochemical analysis of human lymphomas with monoclonal antibodies to B cell and Ia antigens reactive in paraffin sections. Lab Invest. 1985 May;52(5):497–504. [PubMed] [Google Scholar]
  10. Norton A. J., Ramsay A. D., Smith S. H., Beverley P. C., Isaacson P. G. Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986 Apr;39(4):399–405. doi: 10.1136/jcp.39.4.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Salter D. M., Krajewski A. S., Dewar A. E. Immunohistochemical staining of non-Hodgkin's lymphoma with monoclonal antibodies specific for the leucocyte common antigen. J Pathol. 1985 Aug;146(4):345–353. doi: 10.1002/path.1711460408. [DOI] [PubMed] [Google Scholar]
  12. Smith S. H., Brown M. H., Rowe D., Callard R. E., Beverley P. C. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986 May;58(1):63–70. [PMC free article] [PubMed] [Google Scholar]
  13. Stein H., Bonk A., Tolksdorf G., Lennert K., Rodt H., Gerdes J. Immunohistologic analysis of the organization of normal lymphoid tissue and non-Hodgkin's lymphomas. J Histochem Cytochem. 1980 Aug;28(8):746–760. doi: 10.1177/28.8.7003001. [DOI] [PubMed] [Google Scholar]
  14. Stein H., Gerdes J., Mason D. Y. The normal and malignant germinal centre. Clin Haematol. 1982 Oct;11(3):531–559. [PubMed] [Google Scholar]
  15. Warnke R. A., Link M. P. Identification and significance of cell markers in leukemia and lymphoma. Annu Rev Med. 1983;34:117–131. doi: 10.1146/annurev.me.34.020183.001001. [DOI] [PubMed] [Google Scholar]
  16. Warnke R. A., Rouse R. V. Limitations encountered in the application of tissue section immunodiagnosis to the study of lymphomas and related disorders. Hum Pathol. 1985 Apr;16(4):326–331. doi: 10.1016/s0046-8177(85)80226-4. [DOI] [PubMed] [Google Scholar]
  17. Weis J. W., Winter M. W., Phyliky R. L., Banks P. M. Peripheral T-cell lymphomas: histologic, immunohistologic, and clinical characterization. Mayo Clin Proc. 1986 Jun;61(6):411–426. doi: 10.1016/s0025-6196(12)61975-2. [DOI] [PubMed] [Google Scholar]
  18. Weisenburger D. D., Nathwani B. N., Diamond L. W., Winberg C. D., Rappaport H. Malignant lymphoma, intermediate lymphocytic type: a clinicopathologic study of 42 cases. Cancer. 1981 Sep 15;48(6):1415–1425. doi: 10.1002/1097-0142(19810915)48:6<1415::aid-cncr2820480625>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  19. Wood G. S., Link M., Warnke R. A., Dilley J., Levy R. Pan-leukocyte monoclonal antibody L3B12. Characterization and application to research and diagnostic problems. Am J Clin Pathol. 1984 Feb;81(2):176–183. doi: 10.1093/ajcp/81.2.176. [DOI] [PubMed] [Google Scholar]

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