Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1987 Oct;129(1):140–151.

Immunophenotyping of non-Hodgkin's lymphoma. Lack of correlation between immunophenotype and cell morphology.

H J Schuurman 1, J van Baarlen 1, W Huppes 1, B W Lam 1, L F Verdonck 1, J A van Unnik 1
PMCID: PMC1899688  PMID: 3310650

Abstract

The establishment of Clusters of Differentiation for T- and B-lymphoid cells during International Workshops on Human Leukocyte Differentiation Antigens prompted the authors to evaluate the immunophenotypes in 160 cases of non-Hodgkin's lymphoma (NHL). In this group, 130 were of B-lymphocyte lineage (117 by monotypic immunoglobulin expression), and 30 of T-cell lineage. In the B-NHL series the expression of immunoglobulin isotypes, B-cell maturation/differentiation antigens of CD9, CD10, CD19-24, CD37, and CD38 (OKT10), HLA-DR and peanut agglutinin binding showed no significant relationship with histopathologic diagnosis as defined by the Kiel classification. Of the T-cell markers, CD5, CD6, and CD7 showed lineage promiscuity by their presence on some B-NHL. Conversely, the authors grouped the cases according to phenotypes (either CD antigens or immunoglobulin isotypes) which occur in distinct stages of (physiologic) B-cell maturation/differentiation. Eighty-six of the 130 cases could be fitted according to CD phenotype expression. This approach did not yield a significant relationship between phenotype and individual histopathologic categories either. The staging by CD phenotype and by immunoglobulin isotype yielded different results in this respect. Most B-NHL had an intermediate stage of B-cell maturation/differentiation. In the T-NHL series most cases showed a phenotype (CD1-CD8, CD38, TdT, and peanut agglutinin binding capacity) compatible with mature T-lymphocyte characteristics. The exceptions were lymphoblastic convoluted lymphomas, which exhibited an immature immunophenotype. It is concluded that NHL in distinct histopathologic categories are heterogeneous in immunologic phenotypes, and that the immunophenotype of lymphoma cells has no evident association with that of their presumed counterparts in physiologic cell maturation/differentiation.

Full text

PDF
147

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Battifora H., Trowbridge I. S. A monoclonal antibody useful for the differential diagnosis between malignant lymphoma and nonhematopoietic neoplasms. Cancer. 1983 Mar 1;51(5):816–821. doi: 10.1002/1097-0142(19830301)51:5<816::aid-cncr2820510512>3.0.co;2-4. [DOI] [PubMed] [Google Scholar]
  2. Brittinger G., Bartels H., Common H., Dühmke E., Fülle H. H., Gunzer U., Gyenes T., Heinz R., König E., Meusers P. Clinical and prognostic relevance of the Kiel classification of non-Hodgkin lymphomas results of a prospective multicenter study by the Kiel Lymphoma Study Group. Hematol Oncol. 1984 Jul-Sep;2(3):269–306. doi: 10.1002/hon.2900020306. [DOI] [PubMed] [Google Scholar]
  3. Chan L. C., Pegram S. M., Greaves M. F. Contribution of immunophenotype to the classification and differential diagnosis of acute leukaemia. Lancet. 1985 Mar 2;1(8427):475–479. doi: 10.1016/s0140-6736(85)92085-9. [DOI] [PubMed] [Google Scholar]
  4. Cleary M. L., Trela M. J., Weiss L. M., Warnke R., Sklar J. Most null large cell lymphomas are B lineage neoplasms. Lab Invest. 1985 Nov;53(5):521–525. [PubMed] [Google Scholar]
  5. Collins R. D. Lymph node examination. What is an adequate workup? Arch Pathol Lab Med. 1985 Sep;109(9):797–799. [PubMed] [Google Scholar]
  6. Cossman J., Jaffe E. S., Fisher R. I. Immunologic phenotypes of diffuse, aggressive, non-Hodgkin's lymphomas. Correlation with clinical features. Cancer. 1984 Oct 1;54(7):1310–1317. doi: 10.1002/1097-0142(19841001)54:7<1310::aid-cncr2820540714>3.0.co;2-9. [DOI] [PubMed] [Google Scholar]
  7. Cossman J., Neckers L. M., Hsu S., Longo D., Jaffe E. S. Low-grade lymphomas. Expression of developmentally regulated B-cell antigens. Am J Pathol. 1984 Apr;115(1):117–124. [PMC free article] [PubMed] [Google Scholar]
  8. Doggett R. S., Wood G. S., Horning S., Levy R., Dorfman R. F., Bindl J., Warnke R. A. The immunologic characterization of 95 nodal and extranodal diffuse large cell lymphomas in 89 patients. Am J Pathol. 1984 May;115(2):245–252. [PMC free article] [PubMed] [Google Scholar]
  9. Drexler H. G., Menon M., Sagawa K., Tatsumi E., Koshiba H., Koishi T., Minato K., Sugimoto T., Saito M., Morita M. Phenotyping of malignant hematopoietic cells. Analysis of 1200 cases of leukemia-lymphoma. Blut. 1986 Feb;52(2):99–109. doi: 10.1007/BF00321072. [DOI] [PubMed] [Google Scholar]
  10. Foon K. A., Todd R. F., 3rd Immunologic classification of leukemia and lymphoma. Blood. 1986 Jul;68(1):1–31. [PubMed] [Google Scholar]
  11. Freedman A. S., Boyd A. W., Anderson K. C., Fisher D. C., Pinkus G. S., Schlossman S. F., Nadler L. M. Immunologic heterogeneity of diffuse large cell lymphoma. Blood. 1985 Mar;65(3):630–637. [PubMed] [Google Scholar]
  12. GRAHAM R. C., Jr, LUNDHOLM U., KARNOVSKY M. J. CYTOCHEMICAL DEMONSTRATION OF PEROXIDASE ACTIVITY WITH 3-AMINO-9-ETHYLCARBAZOLE. J Histochem Cytochem. 1965 Feb;13:150–152. doi: 10.1177/13.2.150. [DOI] [PubMed] [Google Scholar]
  13. Gerdes J., Dallenbach F., Lennert K., Lemke H., Stein H. Growth fractions in malignant non-Hodgkin's lymphomas (NHL) as determined in situ with the monoclonal antibody Ki-67. Hematol Oncol. 1984 Oct-Dec;2(4):365–371. doi: 10.1002/hon.2900020406. [DOI] [PubMed] [Google Scholar]
  14. Greaves M. F., Chan L. C., Furley A. J., Watt S. M., Molgaard H. V. Lineage promiscuity in hemopoietic differentiation and leukemia. Blood. 1986 Jan;67(1):1–11. [PubMed] [Google Scholar]
  15. Grogan T. M., Hicks M. J., Jolley C. S., Rangel C. S., Jones S. E. Identification of two major B cell forms of nodular mixed lymphoma. Lab Invest. 1984 Nov;51(5):504–514. [PubMed] [Google Scholar]
  16. Haak H. L., Kluin P. M., Meyer C. J., Otter R., Stijnen T., Bieger R., van Groningen K., Kerkhofs H., Noordijk E. M., vd Sandt M. M. Population-based registration of non-Hodgkin lymphoma in the region covered by the Comprehensive Cancer Centre West. The registry of diagnosis and therapy. Neth J Med. 1986;29(4):105–110. [PubMed] [Google Scholar]
  17. Harden E. A., Haynes B. F. Phenotypic and functional characterization of human malignant T cells. Semin Hematol. 1985 Jan;22(1):13–26. [PubMed] [Google Scholar]
  18. Hsu S. M., Jaffe E. S. Phenotypic expression of T lymphocytes in thymus and peripheral lymphoid tissues. Am J Pathol. 1985 Oct;121(1):69–78. [PMC free article] [PubMed] [Google Scholar]
  19. Janossy G., Prentice H. G. T cell subpopulations, monoclonal antibodies and their therapeutic applications. Clin Haematol. 1982 Oct;11(3):631–660. [PubMed] [Google Scholar]
  20. Kansas G. S., Wood G. S., Fishwild D. M., Engleman E. G. Functional characterization of human T lymphocyte subsets distinguished by monoclonal anti-leu-8. J Immunol. 1985 May;134(5):2995–3002. [PubMed] [Google Scholar]
  21. Kluin P. M., de Weger R. A., Schuurman H. J., Peters P. P., Spies P. I., Bast B. J., van Unnik J. A., de Gast G. C. Surface immunoglobulin restriction in B-non Hodgkin's lymphomas in cell suspension and on frozen tissue sections. Scand J Haematol. 1985 Oct;35(4):399–407. doi: 10.1111/j.1600-0609.1985.tb02263.x. [DOI] [PubMed] [Google Scholar]
  22. Knowles D. M., 2nd, Dodson L., Burke J. S., Wang J. M., Bonetti F., Pelicci P. G., Flug F., Dalla-Favera R., Wang C. Y. SIg-E- ("null-cell") non-Hodgkin's lymphomas. Multiparametric determination of their B- or T-cell lineage. Am J Pathol. 1985 Sep;120(3):356–370. [PMC free article] [PubMed] [Google Scholar]
  23. Knowles D. M., 2nd, Halper J. P. Human T-cell malignancies: Correlative clinical, histopathologic, immunologic, and cytochemical analysis of 23 cases. Am J Pathol. 1982 Feb;106(2):187–203. [PMC free article] [PubMed] [Google Scholar]
  24. Mason D. Y., Stein H. Reactive and neoplastic human lymphoid cells producing J chain in the absence of immunoglobulin: evidence for the existence of 'J chain disease'? Clin Exp Immunol. 1981 Nov;46(2):305–312. [PMC free article] [PubMed] [Google Scholar]
  25. Schuurman H. J., Brekelmans P., Daemen T., Broekhuizen R., Kater L. T-cell maturation in the human thymus and tonsil: peanut agglutinin binding T lymphocytes in thymus and tonsil differ in maturation stage. Clin Immunol Immunopathol. 1983 Nov;29(2):271–281. doi: 10.1016/0090-1229(83)90029-6. [DOI] [PubMed] [Google Scholar]
  26. Schuurman H. J., Kluin P. M., de Gast G. C., Kater L. HNK-1+ cells in non-Hodgkin's lymphoma: lack of relation with transferrin receptor expression on malignant cells. Br J Cancer. 1985 Feb;51(2):171–177. doi: 10.1038/bjc.1985.26. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stein H., Gerdes J., Mason D. Y. The normal and malignant germinal centre. Clin Haematol. 1982 Oct;11(3):531–559. [PubMed] [Google Scholar]
  28. Stein H., Lennert K., Feller A. C., Mason D. Y. Immunohistological analysis of human lymphoma: correlation of histological and immunological categories. Adv Cancer Res. 1984;42:67–147. doi: 10.1016/s0065-230x(08)60456-x. [DOI] [PubMed] [Google Scholar]
  29. Warnke R. A., Gatter K. C., Falini B., Hildreth P., Woolston R. E., Pulford K., Cordell J. L., Cohen B., De Wolf-Peeters C., Mason D. Y. Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med. 1983 Nov 24;309(21):1275–1281. doi: 10.1056/NEJM198311243092102. [DOI] [PubMed] [Google Scholar]
  30. Warnke R. A., Rouse R. V. Limitations encountered in the application of tissue section immunodiagnosis to the study of lymphomas and related disorders. Hum Pathol. 1985 Apr;16(4):326–331. doi: 10.1016/s0046-8177(85)80226-4. [DOI] [PubMed] [Google Scholar]
  31. Weiss L. M., Bindl J. M., Picozzi V. J., Link M. P., Warnke R. A. Lymphoblastic lymphoma: an immunophenotype study of 26 cases with comparison to T cell acute lymphoblastic leukemia. Blood. 1986 Feb;67(2):474–478. [PubMed] [Google Scholar]
  32. van de Wiel A., Schuurman H. J., van Riessen D., Haaijman J. J., Radl J., Delacroix D. L., van Hattum J., Blok A. P., Kater L. Characteristics of IgA deposits in liver and skin of patients with liver disease. Am J Clin Pathol. 1986 Dec;86(6):724–730. doi: 10.1093/ajcp/86.6.724. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES