Abstract
The destruction of vascular smooth muscle cells (VSMCs) in autoimmune arteritis is a poorly understood phenomenon. For evaluation of the cellular interactions that may contribute to vasculitis, the immunobiology of VSMCs and lymphocytes was explored in vitro. Primary VSMC cultures were established, and the interaction of these cells (from normal or autoimmune mice) with lymphocytes was then assessed. Specifically, splenocytes from MRL/lpr or C3H mice were cocultivated with MRL/lpr or C3H VSMCs. Massive mononuclear cell clusters from normal and autoimmune mice enveloped MRL/lpr VSMCs, which culminated in the detachment of MRL/lpr VSMCs from the culture plate. In contrast, the interaction of SPs from either normal or autoimmune mice did not encompass or destroy normal VSMCs. Further investigation indicated that MRL/lpr, but not C3H, VSMCs spontaneously expressed Ia and released Il-1 like factor(s), which may be at least two mechanisms by which MRL/lpr VSMCs stimulate the in vitro mononuclear cell influx. As a result of these studies, a novel mechanism for the induction of mononuclear cell autoimmune vasculitis is proposed. VSMCs derived from autoimmune mice may stimulate a mononuclear inflammatory cell phlogistic response which culminates in VSMC autodestruction.
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- Altman A., Theofilopoulos A. N., Weiner R., Katz D. H., Dixon F. J. Analysis of T cell function in autoimmune murine strains. Defects in production and responsiveness to interleukin 2. J Exp Med. 1981 Sep 1;154(3):791–808. doi: 10.1084/jem.154.3.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Andrews B. S., Eisenberg R. A., Theofilopoulos A. N., Izui S., Wilson C. B., McConahey P. J., Murphy E. D., Roths J. B., Dixon F. J. Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestations in several strains. J Exp Med. 1978 Nov 1;148(5):1198–1215. doi: 10.1084/jem.148.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berden J. H., Hang L., McConahey P. J., Dixon F. J. Analysis of vascular lesions in murine SLE. I. Association with serologic abnormalities. J Immunol. 1983 Apr;130(4):1699–1705. [PubMed] [Google Scholar]
- Conn D. L., McDuffie F. C., Holley K. E., Schroeter A. L. Immunologic mechanisms in systemic vasculitis. Mayo Clin Proc. 1976 Aug;51(8):511–518. [PubMed] [Google Scholar]
- Cronin P. S., Sing A. P., Glimcher L. H., Kelley V. E., Reinisch C. L. The isolation and functional characterization of autoimmune clones expressing inappropriate Ia. J Immunol. 1984 Aug;133(2):822–829. [PubMed] [Google Scholar]
- DIXON F. J. THE ROLE OF ANTIGEN-ANTIBODY COMPLEXES IN DISEASE. Harvey Lect. 1963;58:21–52. [PubMed] [Google Scholar]
- Dumont F., Habbersett R. G. Unusual cell surface properties of the T lymphocyte population expanding in MRL/Mp-lpr/lpr mice. Immunology. 1982 Oct;47(2):271–281. [PMC free article] [PubMed] [Google Scholar]
- Dutton R. W., Swain S. L. Regulation of the immune response T-cell interactions. CRC Crit Rev Immunol. 1982 Feb;3(3):209–261. [PubMed] [Google Scholar]
- Gimbrone M. A., Jr, Cotran R. S. Human vascular smooth muscle in culture. Growth and ultrastructure. Lab Invest. 1975 Jul;33(1):16–27. [PubMed] [Google Scholar]
- Gower R. G., Sams W. M., Jr, Thorne E. G., Kohler P. F., Claman H. N. Leukocytoclastic vasculitis: sequential appearance of immunoreactants and cellular changes in serial biopsies. J Invest Dermatol. 1977 Nov;69(5):477–484. doi: 10.1111/1523-1747.ep12511631. [DOI] [PubMed] [Google Scholar]
- Hart M. N., Sadewasser K. L., Cancilla P. A., DeBault L. E. Experimental autoimmune type of vasculitis resulting from activation of mouse lymphocytes to cultured endothelium. Lab Invest. 1983 Apr;48(4):419–427. [PubMed] [Google Scholar]
- Ives H. E., Schultz G. S., Galardy R. E., Jamieson J. D. Preparation of functional smooth muscle cells from the rabbit aorta. J Exp Med. 1978 Nov 1;148(5):1400–1413. doi: 10.1084/jem.148.5.1400. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lewis D. E., Giorgi J. V., Warner N. L. Flow cytometry analysis of T cells and continuous T-cell lines from autoimmune MRL/l mice. Nature. 1981 Jan 22;289(5795):298–300. doi: 10.1038/289298a0. [DOI] [PubMed] [Google Scholar]
- McCluskey R. T., Fienberg R. Vasculitis in primary vasculitides, granulomatoses, and connective tissue diseases. Hum Pathol. 1983 Apr;14(4):305–315. doi: 10.1016/s0046-8177(83)80116-6. [DOI] [PubMed] [Google Scholar]
- Mizel S. B., Rosenstreich D. L. Regulation of lymphocyte-activating factor (LAF) production and secretion in P388D1 cells: identification of high molecular weight precursors of LAF. J Immunol. 1979 Jun;122(6):2173–2179. [PubMed] [Google Scholar]
- Rosenson R. S., Flaherty L., Reinisch C. L. Induction of surface Qa2 on lymphoid cells. J Immunol. 1981 Jun;126(6):2253–2257. [PubMed] [Google Scholar]
- SOKOLOFF L., BUNIM J. J. Vascular lesions in rheumatoid arthritis. J Chronic Dis. 1957 Jun;5(6):668–687. doi: 10.1016/0021-9681(57)90075-9. [DOI] [PubMed] [Google Scholar]
- Soter N. A., Mihm M. C., Jr, Gigli I., Dvorak H. F., Austen K. F. Two distinct cellular patterns in cutaneous necrotizing angiitis. J Invest Dermatol. 1976 Jun;66(6):344–350. doi: 10.1111/1523-1747.ep12482901. [DOI] [PubMed] [Google Scholar]
- Theofilopoulos A. N., Eisenberg R. A., Bourdon M., Crowell J. S., Jr, Dixon F. J. Distribution of lymphocytes identified by surface markers in murine strains with systemic lupus erythematosus-like syndromes. J Exp Med. 1979 Feb 1;149(2):516–534. doi: 10.1084/jem.149.2.516. [DOI] [PMC free article] [PubMed] [Google Scholar]