Abstract
An indirect immunofluorescent method for localizing specific types of collagen was applied to unfixed cryostat sections of autopsy and biopsy specimens of normal and fibrotic human livers. Monospecific antibodies to Types I, III, and B collagens were raised in goats by injecting collagens extracted from normal and fibrotic human livers. The antibody against B collagen stained the delicate sinusoidal meshwork within the lobule that closely paralleled in distribution the staining of reticulin fibers as seen in classical silver preparations. Antibody to Type III collagen stained at the sinusoidal aspect of the hepatocytes in a pattern similar to anti-B antibody. All three antibodies tested strongly stained the portal tracts and the fibrotic bands in specimens with cirrhosis. These observations indicate that reticulin fibers within the liver lobule contain both Type III and Type B collagen.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
- Burgeson R. E., El Adli F. A., Kaitila I. I., Hollister D. W. Fetal membrane collagens: identification of two new collagen alpha chains. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2579–2583. doi: 10.1073/pnas.73.8.2579. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chung E., Rhodes K., Miller E. J. Isolation of three collagenous components of probable basement membrane origin from several tissues. Biochem Biophys Res Commun. 1976 Aug 23;71(4):1167–1174. doi: 10.1016/0006-291x(76)90776-2. [DOI] [PubMed] [Google Scholar]
- Gay S., Fietzek P. P., Remberger K., Eder M., Kühn K. Liver cirrhosis: immunofluorescence and biochemical studies demonstrate two types of collagen. Klin Wochenschr. 1975 Mar 1;53(5):205–208. doi: 10.1007/BF01468808. [DOI] [PubMed] [Google Scholar]
- Hruban Z., Russell R. M., Boyer J. L., Glagov S., Bagheri S. A. Ultrastructural changes in livers of two patients with hypervitaminosis A. Am J Pathol. 1974 Sep;76(3):451–461. [PMC free article] [PubMed] [Google Scholar]
- Kent G., Gay S., Inouye T., Bahu R., Minick O. T., Popper H. Vitamin A-containing lipocytes and formation of type III collagen in liver injury. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3719–3722. doi: 10.1073/pnas.73.10.3719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nowack H., Gay S., Wick G., Becker U., Timpl R. Preparation and use in immunohistology of antibodies specific for type I and type III collagen and procollagen. J Immunol Methods. 1976;12(1-2):117–124. doi: 10.1016/0022-1759(76)90101-0. [DOI] [PubMed] [Google Scholar]
- Oho M., Riquetti P., Hudson B. G. Bovine renal glomerular basement membrane. Isolation and characterization of a glycoprotein component. J Biol Chem. 1975 Oct 10;250(19):7780–7787. [PubMed] [Google Scholar]
- Remberger K., Gay S., Fietzek P. P. Immunohistochemische Untersuchungen zur Kollagencharakterisierung in Lebercirrhosen. Virchows Arch A Pathol Anat Histol. 1975 Aug 12;367(3):231–240. doi: 10.1007/BF00430710. [DOI] [PubMed] [Google Scholar]
- Rhodes R. K., Miller E. J. Physicochemical characterization and molecular organization of the collagen A and B chains. Biochemistry. 1978 Aug 22;17(17):3442–3448. doi: 10.1021/bi00610a003. [DOI] [PubMed] [Google Scholar]
- Rojkind M., Giambrone M. A., Biempica L. Collagen types in normal and cirrhotic liver. Gastroenterology. 1979 Apr;76(4):710–719. [PubMed] [Google Scholar]
- Rojkind M., Martinez-Palomo A. Increase in type I and type III collagens in human alcoholic liver cirrhosis. Proc Natl Acad Sci U S A. 1976 Feb;73(2):539–543. doi: 10.1073/pnas.73.2.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHAFFNER F., POPER H. Capillarization of hepatic sinusoids in man. Gastroenterology. 1963 Mar;44:239–242. [PubMed] [Google Scholar]
- Seyer J. M., Hutcheson E. T., Kang A. H. Collagen polymorphism in normal and cirrhotic human liver. J Clin Invest. 1977 Feb;59(2):241–248. doi: 10.1172/JCI108634. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trelstad R. L., Lawley K. R. Isolation and initial characterization of human basement membrane collagens. Biochem Biophys Res Commun. 1976 May 23;76(2):376–384. doi: 10.1016/0006-291x(77)90735-5. [DOI] [PubMed] [Google Scholar]