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. 1981 Aug;104(2):132–141.

Effects of heparin on platelet aggregation and release and thromboxane A2 production.

S F Mohammad, W H Anderson, J B Smith, H Y Chuang, R G Mason
PMCID: PMC1903756  PMID: 7258300

Abstract

Heparin, when added to citrated platelet-rich plasma (PRP), caused potentiation of platelet aggregation and the release reaction induced by the aggregating agents adenosine diphosphate (ADP), arachidonic acid, collagen, and epinephrine. At low concentrations (4.7 x 10(-5) M) arachidonic acid failed to cause aggregation of platelets in citrated PRP. However, in the presence of heparin, the same concentration of arachidonic acid caused aggregation. Examination of PRP for the presence of thromboxane A2 (TxA2) by use of a bioassay revealed that heparin also stimulated release of TxA2. This finding indicated that platelets released more TxA2 when they were challenged by low concentrations of arachidonic acid in the presence of heparin than in its absence. Platelets were labeled with 3H-arachidonic acid and 14C-serotonin, and attempts were made to determine whether heparin stimulated the platelet release reaction first with subsequent increased production of TxA2, or alternatively, whether heparin stimulated TxA2 production first with subsequent enhancement of the release reaction. In view of the demonstrated simultaneous release of 14C-serotonin and 3H-arachidonic acid metabolites, it appeared that either release of 14C and 3H occurs concurrently or, even if one of these events is dependent on the other, both events take place in rapid succession. Timed sequential studies revealed that in the presence of arachidonic acid, the addition of heparin hastened the apparently simultaneous release of both 14C and 3H.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrahamsen A. F. A modification of the technique for 51Cr-labelling of blood platelets giving increased circulating platelet radioactivity. Scand J Haematol. 1968;5(1):53–63. doi: 10.1111/j.1600-0609.1968.tb01718.x. [DOI] [PubMed] [Google Scholar]
  2. Anderson W. H., Mohammad S. F., Chuang H. Y., Mason R. G. Heparin potentiates synthesis of thromboxane A2 in human platelets. Adv Prostaglandin Thromboxane Res. 1980;6:287–291. [PubMed] [Google Scholar]
  3. Ansell J., Deykin D. Heparin-induced thrombocytopenia and recurrent thromboembolism. Am J Hematol. 1980;8(3):325–332. doi: 10.1002/ajh.2830080311. [DOI] [PubMed] [Google Scholar]
  4. Bell W. R., Royall R. M. Heparin-associated thrombocytopenia: a comparison of three heparin preparations. N Engl J Med. 1980 Oct 16;303(16):902–907. doi: 10.1056/NEJM198010163031602. [DOI] [PubMed] [Google Scholar]
  5. Bell W. R., Tomasulo P. A., Alving B. M., Duffy T. P. Thrombocytopenia occurring during the administration of heparin. A prospective study in 52 patients. Ann Intern Med. 1976 Aug;85(2):155–160. doi: 10.7326/0003-4819-85-2-155. [DOI] [PubMed] [Google Scholar]
  6. Besterman E. M., Gillett M. P. Heparin effects on plasma lysolecithin formation and platelet aggregation. Atherosclerosis. 1973 May-Jun;17(3):503–513. doi: 10.1016/0021-9150(73)90040-3. [DOI] [PubMed] [Google Scholar]
  7. Eika C. On the mechanism of platelet aggregation induced by heparin, protamine and polybrene. Scand J Haematol. 1972;9(3):248–257. doi: 10.1111/j.1600-0609.1972.tb00937.x. [DOI] [PubMed] [Google Scholar]
  8. Fratantoni J. C., Pollet R., Gralnick H. R. Heparin-induced thrombocytopenia: confirmation of diagnosis with in vitro methods. Blood. 1975 Mar;45(3):395–401. [PubMed] [Google Scholar]
  9. Godal H. C. Report of the International Committee on Thrombosis and Haemostasis. Thrombocytopenia and heparin. Thromb Haemost. 1980 Jul 15;43(3):222–224. [PubMed] [Google Scholar]
  10. Halushka P. V., Knapp D. R., Grimm L. Prostaglandins, thromboxanes, and platelet function. Curr Top Hematol. 1979;2:75–143. [PubMed] [Google Scholar]
  11. Jubiz W., Frailey J., Child C., Bartholomew K. Physiologic role of prostaglandins of the E(PGE), F(PGF) and AB(PGAB) groups. Estimation by radioimmunoassay in unextracted human plasma. Prostaglandins. 1972 Dec;2(6):471–489. doi: 10.1016/s0090-6980(72)80034-0. [DOI] [PubMed] [Google Scholar]
  12. Kunze H., Vogt W. Significance of phospholipase A for prostaglandin formation. Ann N Y Acad Sci. 1971 Apr 30;180:123–125. doi: 10.1111/j.1749-6632.1971.tb53191.x. [DOI] [PubMed] [Google Scholar]
  13. Mohammad S. F., Reddick R. L., Mason R. G. Characterization of human platelets separated from blood by ADP-induced aggregation. Am J Pathol. 1975 Apr;79(1):81–94. [PMC free article] [PubMed] [Google Scholar]
  14. Mohammed S. F., Whitworth C., Chuang H. Y., Lundblad R. L., Mason R. G. Multiple active forms of thrombin: binding to platelets and effects on platelet function. Proc Natl Acad Sci U S A. 1976 May;73(5):1660–1663. doi: 10.1073/pnas.73.5.1660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Needleman P., Minkes M., Raz A. Thromboxanes: selective biosynthesis and distinct biological properties. Science. 1976 Jul 9;193(4248):163–165. doi: 10.1126/science.945611. [DOI] [PubMed] [Google Scholar]
  16. Olivecrona T., Bengtsson G., Marklund S. E., Lindahl U., Hök M. Heparin-lipoprotein lipase interactions. Fed Proc. 1977 Jan;36(1):60–65. [PubMed] [Google Scholar]
  17. Piper P. J., Vane J. R. Release of additional factors in anaphylaxis and its antagonism by anti-inflammatory drugs. Nature. 1969 Jul 5;223(5201):29–35. doi: 10.1038/223029a0. [DOI] [PubMed] [Google Scholar]
  18. Rhodes G. R., Dixon R. H., Silver D. Heparin induced thrombocytopenia with thrombotic and hemorrhagic manifestations. Surg Gynecol Obstet. 1973 Mar;136(3):409–416. [PubMed] [Google Scholar]
  19. Rowsell H. C., Glynn M. F., Mustard J. F., Murphy E. A. Effect of heparin on platelet economy in dogs. Am J Physiol. 1967 Oct;213(4):915–922. doi: 10.1152/ajplegacy.1967.213.4.915. [DOI] [PubMed] [Google Scholar]
  20. Zucker M. B. Effect of heparin on platelet function. Thromb Diath Haemorrh. 1975 Feb 28;33(1):63–65. [PubMed] [Google Scholar]
  21. Zucker M. B. Heparin and platelet function. Fed Proc. 1977 Jan;36(1):47–49. [PubMed] [Google Scholar]

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