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. 1981 Nov;105(2):97–106.

Comparison of histologic and immunologic heterogeneity of non-Hodgkin's lymphomas.

M J Borowitz, B P Croker, R S Metzgar
PMCID: PMC1903885  PMID: 6170233

Abstract

The lymphocyte surface marker phenotype in 11 selected cases of non-Hodgkin's lymphomas was determined with anti-immunoglobulin and mouse monoclonal antibodies against human lymphocyte antigens. A complement-mediated cell cytotoxicity assay on suspensions of the tumor cells was compared with an indirect immunoperoxidase technique on frozen tissue sections. Both methods gave good results in tumors with a uniform cell population, but the frozen section technique was superior in heterogeneous tumors. The six B-cell neoplasms were heterogeneous with respect to expression of surface immunoglobulin and Ia antigens. The five T-cell tumors were morphologically heterogeneous and also highly variable in their expression of different T-cell specific antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. K., Metzgar R. S. Detection and partial characterization of human T and B lymphocyte membrane antigens with antisera to HSB and SB cell lines. J Immunol. 1978 Jan;120(1):262–271. [PubMed] [Google Scholar]
  2. Bernard A., Boumsell L., Bayle C., Richard Y., Coppin H., Penit C., Rouget P., Micheau C., Clausse B., Gerard-Marchant R. Subsets of malignant lymphomas in children related to the cell phenotype. Blood. 1979 Nov;54(5):1058–1068. [PubMed] [Google Scholar]
  3. Bhan A. K., Reinherz E. L., Poppema S., McCluskey R. T., Schlossman S. F. Location of T cell and major histocompatibility complex antigens in the human thymus. J Exp Med. 1980 Oct 1;152(4):771–782. doi: 10.1084/jem.152.4.771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradstock K. F., Janossy G., Pizzolo G., Hoffbrand A. V., McMichael A., Pilch J. R., Milstein C., Beverley P., Bollum F. J. Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies. J Natl Cancer Inst. 1980 Jul;65(1):33–42. [PubMed] [Google Scholar]
  5. Collins R. D., Waldron J. A., Glick A. D. Results of multiparameter studies of T-cell lymphoid neoplasms. Am J Clin Pathol. 1979 Oct;72(4 Suppl):699–707. [PubMed] [Google Scholar]
  6. Dowell B. L., Falletta J. M., Moore J. O., Metzgar R. S. Detection and partial characterization of human thymus-leukemia antigens. J Natl Cancer Inst. 1980 Oct;65(4):691–701. doi: 10.1093/jnci/65.4.691. [DOI] [PubMed] [Google Scholar]
  7. Halper J. P., Knowles D. M., 2nd, Wang C. Y. Ia antigen expression by human malignant lymphomas: correlation with conventional lymphoid markers. Blood. 1980 Mar;55(3):373–382. [PubMed] [Google Scholar]
  8. Haynes B. F. Human T lymphocyte antigens as defined by monoclonal antibodies. Immunol Rev. 1981;57:127–161. doi: 10.1111/j.1600-065x.1981.tb00445.x. [DOI] [PubMed] [Google Scholar]
  9. Haynes B. F., Mann D. L., Hemler M. E., Schroer J. A., Shelhamer J. H., Eisenbarth G. S., Strominger J. L., Thomas C. A., Mostowski H. S., Fauci A. S. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc Natl Acad Sci U S A. 1980 May;77(5):2914–2918. doi: 10.1073/pnas.77.5.2914. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Haynes B. F., Metzgar R. S., Minna J. D., Bunn P. A. Phenotypic characterization of cutaneous T-cell lymphoma. Use of monoclonal antibodies to compare with other malignant T cells. N Engl J Med. 1981 May 28;304(22):1319–1323. doi: 10.1056/NEJM198105283042202. [DOI] [PubMed] [Google Scholar]
  11. Janossy G., Hoffbrand A. V., Greaves M. F., Ganeshaguru K., Pain C., Bradstock K. F., Prentice H. G., Kay H. E., Lister T. A. Terminal transferase enzyme assay and immunological membrane markers in the diagnosis of leukaemia: a multiparameter analysis of 300 cases. Br J Haematol. 1980 Feb;44(2):221–234. doi: 10.1111/j.1365-2141.1980.tb01204.x. [DOI] [PubMed] [Google Scholar]
  12. Janossy G., Tidman N., Selby W. S., Thomas J. A., Granger S., Kung P. C., Goldstein G. Human T lymphocytes of inducer and suppressor type occupy different microenvironments. Nature. 1980 Nov 6;288(5786):81–84. doi: 10.1038/288081a0. [DOI] [PubMed] [Google Scholar]
  13. Ko H. S., Fu S. M., Winchester R. J., Yu D. T., Kunkel H. G. Ia determinants on stimulated human T lymphocytes. Occurrence on mitogen- and antigen-activated T cells. J Exp Med. 1979 Aug 1;150(2):246–255. doi: 10.1084/jem.150.2.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Levy R., Dilley J., Fox R. I., Warnke R. A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6552–6556. doi: 10.1073/pnas.76.12.6552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lukes R. J., Parker J. W., Taylor C. R., Tindle B. H., Cramer A. D., Lincoln T. L. Immunologic approach to non-Hodgkin lymphomas and related leukemias. Analysis of the results of multiparameter studies of 425 cases. Semin Hematol. 1978 Oct;15(4):322–351. [PubMed] [Google Scholar]
  16. Mann R. B., Jaffe E. S., Berard C. W. Malignant lymphomas--a conceptual understanding of morphologic diversity. A review. Am J Pathol. 1979 Jan;94(1):105–192. [PMC free article] [PubMed] [Google Scholar]
  17. Metzgar R. S., Bertoglio J., Anderson J. K., Bonnard G. D., Ruscetti F. W. Detection of HLA-DRw (Ia-like) antigens on human T lymphocytes grown in tissue culture. J Immunol. 1979 Mar;122(3):949–953. [PubMed] [Google Scholar]
  18. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  19. Pizzolo G., Sloane J., Beverley P., Thomas J. A., Bradstock K. F., Mattingly S., Janossy G. Differential diagnosis of malignant lymphoma and nonlymphoid tumors using monoclonal anti-leucocyte antibody. Cancer. 1980 Dec 15;46(12):2640–2647. doi: 10.1002/1097-0142(19801215)46:12<2640::aid-cncr2820461218>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  20. Poppema S., Bhan A. K., Reinherz E. L., McCluskey R. T., Schlossman S. F. Distribution of T cell subsets in human lymph nodes. J Exp Med. 1981 Jan 1;153(1):30–41. doi: 10.1084/jem.153.1.30. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reinherz E. L., Kung P. C., Goldstein G., Levey R. H., Schlossman S. F. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1588–1592. doi: 10.1073/pnas.77.3.1588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Reinherz E. L., Nadler L. M., Rosenthal D. S., Moloney W. C., Schlossman S. F. T-cell-subset characterization of human T-CLL. Blood. 1979 Jun;53(6):1066–1075. [PubMed] [Google Scholar]
  23. Stein H., Bonk A., Tolksdorf G., Lennert K., Rodt H., Gerdes J. Immunohistologic analysis of the organization of normal lymphoid tissue and non-Hodgkin's lymphomas. J Histochem Cytochem. 1980 Aug;28(8):746–760. doi: 10.1177/28.8.7003001. [DOI] [PubMed] [Google Scholar]
  24. Thiel E., Rodt H., Huhn D., Netzel B., Grosse-Wilde H., Ganeshaguru K., Thierfelder S. Multimarker classification of acute lymphoblastic leukemia: evidence for further T subgroups and evaluation of their clinical significance. Blood. 1980 Nov;56(5):759–772. [PubMed] [Google Scholar]
  25. Waldron J. A., Leech J. H., Glick A. D., Flexner J. M., Collins R. D. Malignant lymphoma of peripheral T-lymphocyte origin: immunologic, pathologic, and clinical features in six patients. Cancer. 1977 Oct;40(4):1604–1617. doi: 10.1002/1097-0142(197710)40:4<1604::aid-cncr2820400433>3.0.co;2-h. [DOI] [PubMed] [Google Scholar]
  26. Warnke R., Levy R. Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method. J Histochem Cytochem. 1980 Aug;28(8):771–776. doi: 10.1177/28.8.7003003. [DOI] [PubMed] [Google Scholar]
  27. Warnke R., Levy R. Immunopathology of follicular lymphomas. A model of B-lymphocyte homing. N Engl J Med. 1978 Mar 2;298(9):481–486. doi: 10.1056/NEJM197803022980903. [DOI] [PubMed] [Google Scholar]
  28. Warnke R., Miller R., Grogan T., Pederson M., Dilley J., Levy R. Immunologic phenotype in 30 patients with diffuse large-cell lymphoma. N Engl J Med. 1980 Aug 7;303(6):293–300. doi: 10.1056/NEJM198008073030601. [DOI] [PubMed] [Google Scholar]
  29. Watanabe S., Shimosato Y., Shimoyama M., Minato K., Suzuki M., Abe M., Nagatani T. Adult T cell lymphoma with hypergammaglobulinemia. Cancer. 1980 Dec 1;46(11):2472–2483. doi: 10.1002/1097-0142(19801201)46:11<2472::aid-cncr2820461128>3.0.co;2-z. [DOI] [PubMed] [Google Scholar]
  30. Yamanaka N., Ishii Y., Koshiba H., Mikuni C., Ogasawara M., Kikuchi K. A study of surface markers in non-Hodgkin's lymphoma by using anti-T and anti-B lymphocyte sera. Cancer. 1981 Jan 15;47(2):311–318. doi: 10.1002/1097-0142(19810115)47:2<311::aid-cncr2820470217>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]

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