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. 1991 Oct;104(2):565–569. doi: 10.1111/j.1476-5381.1991.tb12469.x

Endogenous nitric oxide modulates adrenergic neural vasoconstriction in guinea-pig pulmonary artery.

S F Liu 1, D E Crawley 1, T W Evans 1, P J Barnes 1
PMCID: PMC1908560  PMID: 1686738

Abstract

1. Electrical field stimulation (EFS) of guinea-pig isolated pulmonary artery induced a frequency-dependent contraction. This was abolished by tetrodotoxin (1 microM) and prevented by phentolamine and prazosin (both 1 microM), indicating a role for alpha 1-adrenoceptors activated by noradrenaline (NA) released from perivascular adrenergic nerves. 2. L-NG-monomethyl arginine (L-NMMA, 0.3-100 microM) caused a concentration-dependent enhancement of the EFS-induced contraction with a 3.4 +/- 0.5 fold increase at 100 microM n = 6). The augmenting effect of 30 microM L-NMMA on the contraction to EFS was completely reversed by 100-300 microM L-arginine, but not by an identical concentration of D-arginine. 3. The contractile response to exogenous NA was similarly enhanced by 30 microM L-NMMA (2.9 +/- 0.6 fold increase, n = 5). 4. The contractile responses to exogenous phenylephrine and prostaglandin F2 alpha while matched the contraction to EFS (4 Hz) were equally augmented by 30 microM L-NMMA. 5. In vessel rings submaximally contracted with the thromboxane analogue U44069 (2 microM), the selective alpha 2-adrenoceptor agonist UK14304 induced concentration-dependent relaxation, which was abolished by removal of endothelium. NA had little relaxant effect on these precontracted vessel rings unless in the presence of prazosin (1 microM). 6. Indomethacin had no significant effect on the contractile response to EFS or NA, indicating that vasodilator cyclo-oxygenase products such as prostacyclin are not involved in modulating these responses.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Angus J. A., Cocks T. M., Satoh K. Alpha 2-adrenoceptors and endothelium-dependent relaxation in canine large arteries. Br J Pharmacol. 1986 Aug;88(4):767–777. doi: 10.1111/j.1476-5381.1986.tb16249.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bullock G. R., Taylor S. G., Weston A. H. Influence of the vascular endothelium on agonist-induced contractions and relaxations in rat aorta. Br J Pharmacol. 1986 Dec;89(4):819–830. doi: 10.1111/j.1476-5381.1986.tb11187.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carrier G. O., White R. E. Enhancement of alpha-1 and alpha-2 adrenergic agonist-induced vasoconstriction by removal of endothelium in rat aorta. J Pharmacol Exp Ther. 1985 Mar;232(3):682–687. [PubMed] [Google Scholar]
  4. Cocks T. M., Angus J. A. Endothelium-dependent relaxation of coronary arteries by noradrenaline and serotonin. Nature. 1983 Oct 13;305(5935):627–630. doi: 10.1038/305627a0. [DOI] [PubMed] [Google Scholar]
  5. Cohen R. A., Weisbrod R. M. Endothelium inhibits norepinephrine release from adrenergic nerves of rabbit carotid artery. Am J Physiol. 1988 May;254(5 Pt 2):H871–H878. doi: 10.1152/ajpheart.1988.254.5.H871. [DOI] [PubMed] [Google Scholar]
  6. De Mey J. G., Claeys M., Vanhoutte P. M. Endothelium-dependent inhibitory effects of acetylcholine, adenosine triphosphate, thrombin and arachidonic acid in the canine femoral artery. J Pharmacol Exp Ther. 1982 Jul;222(1):166–173. [PubMed] [Google Scholar]
  7. Eglème C., Godfraind T., Miller R. C. Enhanced responsiveness of rat isolated aorta to clonidine after removal of the endothelial cells. Br J Pharmacol. 1984 Jan;81(1):16–18. doi: 10.1111/j.1476-5381.1984.tb10736.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Furchgott R. F. The role of endothelium in the responses of vascular smooth muscle to drugs. Annu Rev Pharmacol Toxicol. 1984;24:175–197. doi: 10.1146/annurev.pa.24.040184.001135. [DOI] [PubMed] [Google Scholar]
  9. Gillespie J. S., Liu X. R., Martin W. The effects of L-arginine and NG-monomethyl L-arginine on the response of the rat anococcygeus muscle to NANC nerve stimulation. Br J Pharmacol. 1989 Dec;98(4):1080–1082. doi: 10.1111/j.1476-5381.1989.tb12650.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hynes M. R., Dang H., Duckles S. P. Contractile responses to adrenergic nerve stimulation are enhanced with removal of endothelium in rat caudal artery. Life Sci. 1988;42(4):357–365. doi: 10.1016/0024-3205(88)90073-2. [DOI] [PubMed] [Google Scholar]
  11. Ignarro L. J., Byrns R. E., Buga G. M., Wood K. S. Endothelium-derived relaxing factor from pulmonary artery and vein possesses pharmacologic and chemical properties identical to those of nitric oxide radical. Circ Res. 1987 Dec;61(6):866–879. doi: 10.1161/01.res.61.6.866. [DOI] [PubMed] [Google Scholar]
  12. Johns R. A., Peach M. J., Linden J., Tichotsky A. NG-monomethyl L-arginine inhibits endothelium-derived relaxing factor-stimulated cyclic GMP accumulation in cocultures of endothelial and vascular smooth muscle cells by an action specific to the endothelial cell. Circ Res. 1990 Oct;67(4):979–985. doi: 10.1161/01.res.67.4.979. [DOI] [PubMed] [Google Scholar]
  13. Li C. G., Rand M. J. Evidence that part of the NANC relaxant response of guinea-pig trachea to electrical field stimulation is mediated by nitric oxide. Br J Pharmacol. 1991 Jan;102(1):91–94. doi: 10.1111/j.1476-5381.1991.tb12137.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Liu S. F., Crawley D. E., Barnes P. J., Evans T. W. Endothelium-derived relaxing factor inhibits hypoxic pulmonary vasoconstriction in rats. Am Rev Respir Dis. 1991 Jan;143(1):32–37. doi: 10.1164/ajrccm/143.1.32. [DOI] [PubMed] [Google Scholar]
  15. Lues I., Schümann H. J. Effect of removing the endothelial cells on the reactivity of rat aortic segments to different alpha-adrenoceptor agonists. Naunyn Schmiedebergs Arch Pharmacol. 1984 Dec;328(2):160–163. doi: 10.1007/BF00512066. [DOI] [PubMed] [Google Scholar]
  16. Martin W., Furchgott R. F., Villani G. M., Jothianandan D. Depression of contractile responses in rat aorta by spontaneously released endothelium-derived relaxing factor. J Pharmacol Exp Ther. 1986 May;237(2):529–538. [PubMed] [Google Scholar]
  17. Moncada S., Palmer R. M., Higgs E. A. Biosynthesis of nitric oxide from L-arginine. A pathway for the regulation of cell function and communication. Biochem Pharmacol. 1989 Jun 1;38(11):1709–1715. doi: 10.1016/0006-2952(89)90403-6. [DOI] [PubMed] [Google Scholar]
  18. Moncada S., Palmer R. M., Higgs E. A. The discovery of nitric oxide as the endogenous nitrovasodilator. Hypertension. 1988 Oct;12(4):365–372. doi: 10.1161/01.hyp.12.4.365. [DOI] [PubMed] [Google Scholar]
  19. Palmer R. M., Ashton D. S., Moncada S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature. 1988 Jun 16;333(6174):664–666. doi: 10.1038/333664a0. [DOI] [PubMed] [Google Scholar]
  20. Palmer R. M., Ferrige A. G., Moncada S. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature. 1987 Jun 11;327(6122):524–526. doi: 10.1038/327524a0. [DOI] [PubMed] [Google Scholar]
  21. Palmer R. M., Rees D. D., Ashton D. S., Moncada S. L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation. Biochem Biophys Res Commun. 1988 Jun 30;153(3):1251–1256. doi: 10.1016/s0006-291x(88)81362-7. [DOI] [PubMed] [Google Scholar]
  22. Rees D. D., Palmer R. M., Moncada S. Role of endothelium-derived nitric oxide in the regulation of blood pressure. Proc Natl Acad Sci U S A. 1989 May;86(9):3375–3378. doi: 10.1073/pnas.86.9.3375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sata T., Misra H. P., Kubota E., Said S. I. Vasoactive intestinal polypeptide relaxes pulmonary artery by an endothelium-independent mechanism. Peptides. 1986;7 (Suppl 1):225–227. doi: 10.1016/0196-9781(86)90190-7. [DOI] [PubMed] [Google Scholar]
  24. Schmidt H. H., Nau H., Wittfoht W., Gerlach J., Prescher K. E., Klein M. M., Niroomand F., Böhme E. Arginine is a physiological precursor of endothelium-derived nitric oxide. Eur J Pharmacol. 1988 Sep 13;154(2):213–216. doi: 10.1016/0014-2999(88)90101-x. [DOI] [PubMed] [Google Scholar]
  25. Shaul P. W., Muntz K. H., Buja L. M. Comparison of beta adrenergic receptor binding characteristics and coupling to adenylate cyclase in rat pulmonary artery versus aorta. J Pharmacol Exp Ther. 1990 Jan;252(1):86–92. [PubMed] [Google Scholar]
  26. Staub N. C. Site of hypoxic pulmonary vasoconstriction. Chest. 1985 Oct;88(4 Suppl):240S–245S. doi: 10.1378/chest.88.4_supplement.240s. [DOI] [PubMed] [Google Scholar]
  27. Tesfamariam B., Cohen R. A. Inhibition of adrenergic vasoconstriction by endothelial cell shear stress. Circ Res. 1988 Oct;63(4):720–725. doi: 10.1161/01.res.63.4.720. [DOI] [PubMed] [Google Scholar]
  28. Tesfamariam B., Halpern W. Modulation of adrenergic responses in pressurized resistance arteries by flow. Am J Physiol. 1987 Nov;253(5 Pt 2):H1112–H1119. doi: 10.1152/ajpheart.1987.253.5.H1112. [DOI] [PubMed] [Google Scholar]
  29. Tesfamariam B., Weisbrod R. M., Cohen R. A. Endothelium inhibits responses of rabbit carotid artery to adrenergic nerve stimulation. Am J Physiol. 1987 Oct;253(4 Pt 2):H792–H798. doi: 10.1152/ajpheart.1987.253.4.H792. [DOI] [PubMed] [Google Scholar]
  30. Toda N., Okamura T. Mechanism underlying the response to vasodilator nerve stimulation in isolated dog and monkey cerebral arteries. Am J Physiol. 1990 Nov;259(5 Pt 2):H1511–H1517. doi: 10.1152/ajpheart.1990.259.5.H1511. [DOI] [PubMed] [Google Scholar]
  31. Tucker J. F., Brave S. R., Charalambous L., Hobbs A. J., Gibson A. L-NG-nitro arginine inhibits non-adrenergic, non-cholinergic relaxations of guinea-pig isolated tracheal smooth muscle. Br J Pharmacol. 1990 Aug;100(4):663–664. doi: 10.1111/j.1476-5381.1990.tb14072.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Vanhoutte P. M., Miller V. M. Alpha 2-adrenoceptors and endothelium-derived relaxing factor. Am J Med. 1989 Sep 18;87(3C):1S–5S. doi: 10.1016/0002-9343(89)90496-8. [DOI] [PubMed] [Google Scholar]
  33. Wiklund N. P., Persson M. G., Gustafsson L. E., Moncada S., Hedqvist P. Modulatory role of endogenous nitric oxide in pulmonary circulation in vivo. Eur J Pharmacol. 1990 Aug 21;185(1):123–124. doi: 10.1016/0014-2999(90)90221-q. [DOI] [PubMed] [Google Scholar]

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