Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1992 Jan;105(1):135–141. doi: 10.1111/j.1476-5381.1992.tb14224.x

Endothelin-1-induced [3H]-inositol phosphate accumulation in rat trachea.

P J Henry 1, P J Rigby 1, G J Self 1, J M Preuss 1, R G Goldie 1
PMCID: PMC1908599  PMID: 1596675

Abstract

1. The effects of endothelin-1 (ET-1) and of the muscarinic cholinoceptor agonist, carbachol, on [3H]-inositol phosphate ([3H]-InsP) accumulation and smooth muscle contraction were determined in rat isolated tracheal tissue. 2. ET-1 (1 microM) and carbachol (10 microM) induced significant accumulation of [3H]-InsPs in myo-[2-3H]-inositol-loaded rat tracheal segments. Several components of the tracheal wall including the airway smooth muscle band, the cartilaginous region and the intercartilaginous region generated significant levels of [3H]-InsPs in response to ET-1 and carbachol. Following stimulation with ET-1, a greater proportion of tracheal [3H]-InsPs were generated in the intercartilaginous region (49%) than in either the airway smooth muscle band (25%) or cartilaginous region (26%). However, when the respective weights of these regions is taken into account, ET-1-induced accumulation of [3H]-InsPs was greatest in the airway smooth muscle band. The tracheal epithelium did not appear to generate [3H]-InsPs in response to ET-1 or modulate either basal or ET-1-induced accumulation of [3H]-InsPs in rat tracheal segments. 3. In the rat tracheal smooth muscle band, ET-1 caused a time- and concentration-dependent accumulation of [3H]-InsPs. Concentrations of ET-1 as low as 10 nM produced significant accumulation of [3H]-InsPs (1.23 +/- 0.10 fold increase above basal levels of 295 +/- 2 d.p.m. mg-1 wet wt., n = 3 experiments).(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
135

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Advenier C., Sarria B., Naline E., Puybasset L., Lagente V. Contractile activity of three endothelins (ET-1, ET-2 and ET-3) on the human isolated bronchus. Br J Pharmacol. 1990 May;100(1):168–172. doi: 10.1111/j.1476-5381.1990.tb12071.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bainbridge T., Feldman R. D., Welsh M. J. Adrenergic stimulation of inositol phosphate accumulation in tracheal epithelium. J Appl Physiol (1985) 1989 Jan;66(1):504–508. doi: 10.1152/jappl.1989.66.1.504. [DOI] [PubMed] [Google Scholar]
  3. Baron C. B., Cunningham M., Strauss J. F., 3rd, Coburn R. F. Pharmacomechanical coupling in smooth muscle may involve phosphatidylinositol metabolism. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6899–6903. doi: 10.1073/pnas.81.21.6899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Battistini B., Filep J., Sirois P. Potent thromboxane-mediated in vitro bronchoconstrictor effect of endothelin in the guinea-pig. Eur J Pharmacol. 1990 Mar 13;178(1):141–142. doi: 10.1016/0014-2999(90)94808-b. [DOI] [PubMed] [Google Scholar]
  5. Battistini B., Sirois P., Braquet P., Filep J. G. Endothelin-induced constriction of guinea pig airways: role of platelet-activating factor. Eur J Pharmacol. 1990 Sep 21;186(2-3):307–310. doi: 10.1016/0014-2999(90)90449-g. [DOI] [PubMed] [Google Scholar]
  6. Berridge M. J., Downes C. P., Hanley M. R. Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands. Biochem J. 1982 Sep 15;206(3):587–595. doi: 10.1042/bj2060587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Black P. N., Ghatei M. A., Takahashi K., Bretherton-Watt D., Krausz T., Dollery C. T., Bloom S. R. Formation of endothelin by cultured airway epithelial cells. FEBS Lett. 1989 Sep 11;255(1):129–132. doi: 10.1016/0014-5793(89)81075-0. [DOI] [PubMed] [Google Scholar]
  8. Chilvers E. R., Barnes P. J., Nahorski S. R. Characterization of agonist-stimulated incorporation of myo-[3H]inositol into inositol phospholipids and [3H]inositol phosphate formation in tracheal smooth muscle. Biochem J. 1989 Sep 15;262(3):739–746. doi: 10.1042/bj2620739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chilvers E. R., Batty I. H., Barnes P. J., Nahorski S. R. Formation of inositol polyphosphates in airway smooth muscle after muscarinic receptor stimulation. J Pharmacol Exp Ther. 1990 Feb;252(2):786–791. [PubMed] [Google Scholar]
  10. Chilvers E. R., Nahorski S. R. Phosphoinositide metabolism in airway smooth muscle. Am Rev Respir Dis. 1990 Mar;141(3 Pt 2):S137–S140. doi: 10.1164/ajrccm/141.3_Pt_2.S137. [DOI] [PubMed] [Google Scholar]
  11. Fukuda Y., Hirata Y., Yoshimi H., Kojima T., Kobayashi Y., Yanagisawa M., Masaki T. Endothelin is a potent secretagogue for atrial natriuretic peptide in cultured rat atrial myocytes. Biochem Biophys Res Commun. 1988 Aug 30;155(1):167–172. doi: 10.1016/s0006-291x(88)81064-7. [DOI] [PubMed] [Google Scholar]
  12. Goldie R. G., Papadimitriou J. M., Paterson J. W., Rigby P. J., Self H. M., Spina D. Influence of the epithelium on responsiveness of guinea-pig isolated trachea to contractile and relaxant agonists. Br J Pharmacol. 1986 Jan;87(1):5–14. doi: 10.1111/j.1476-5381.1986.tb10150.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Grandordy B. M., Barnes P. J. Airway smooth muscle and disease workshop: phosphoinositide turnover. Am Rev Respir Dis. 1987 Oct;136(4 Pt 2):S17–S20. doi: 10.1164/ajrccm/136.4_Pt_2.S17. [DOI] [PubMed] [Google Scholar]
  14. Grandordy B. M., Cuss F. M., Sampson A. S., Palmer J. B., Barnes P. J. Phosphatidylinositol response to cholinergic agonists in airway smooth muscle: relationship to contraction and muscarinic receptor occupancy. J Pharmacol Exp Ther. 1986 Jul;238(1):273–279. [PubMed] [Google Scholar]
  15. Grandordy B. M., Frossard N., Rhoden K. J., Barnes P. J. Tachykinin-induced phosphoinositide breakdown in airway smooth muscle and epithelium: relationship to contraction. Mol Pharmacol. 1988 May;33(5):515–519. [PubMed] [Google Scholar]
  16. Hashimoto T., Hirata M., Ito Y. A role for inositol 1,4,5-trisphosphate in the initiation of agonist-induced contractions of dog tracheal smooth muscle. Br J Pharmacol. 1985 Sep;86(1):191–199. doi: 10.1111/j.1476-5381.1985.tb09449.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hay D. W. Mechanism of endothelin-induced contraction in guinea-pig trachea: comparison with rat aorta. Br J Pharmacol. 1990 Jun;100(2):383–392. doi: 10.1111/j.1476-5381.1990.tb15814.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hemsén A., Franco-Cereceda A., Matran R., Rudehill A., Lundberg J. M. Occurrence, specific binding sites and functional effects of endothelin in human cardiopulmonary tissue. Eur J Pharmacol. 1990 Dec 4;191(3):319–328. doi: 10.1016/0014-2999(90)94164-s. [DOI] [PubMed] [Google Scholar]
  19. Henry P. J., Rigby P. J., Self G. J., Preuss J. M., Goldie R. G. Relationship between endothelin-1 binding site densities and constrictor activities in human and animal airway smooth muscle. Br J Pharmacol. 1990 Aug;100(4):786–792. doi: 10.1111/j.1476-5381.1990.tb14093.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hwang P. M., Bredt D. S., Snyder S. H. Autoradiographic imaging of phosphoinositide turnover in the brain. Science. 1990 Aug 17;249(4970):802–804. doi: 10.1126/science.1975122. [DOI] [PubMed] [Google Scholar]
  21. Ishikawa T., Yanagisawa M., Kimura S., Goto K., Masaki T. Positive inotropic action of novel vasoconstrictor peptide endothelin on guinea pig atria. Am J Physiol. 1988 Oct;255(4 Pt 2):H970–H973. doi: 10.1152/ajpheart.1988.255.4.H970. [DOI] [PubMed] [Google Scholar]
  22. Katsuyama H., Suzuki S., Nishiye E. Actions of second messengers synthesized by various spasmogenic agents and their relation to mechanical responses in dog tracheal smooth muscle. Br J Pharmacol. 1990 May;100(1):41–48. doi: 10.1111/j.1476-5381.1990.tb12049.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kotlikoff M. I. Calcium currents in isolated canine airway smooth muscle cells. Am J Physiol. 1988 Jun;254(6 Pt 1):C793–C801. doi: 10.1152/ajpcell.1988.254.6.C793. [DOI] [PubMed] [Google Scholar]
  24. Lagente V., Chabrier P. E., Mencia-Huerta J. M., Braquet P. Pharmacological modulation of the bronchopulmonary action of the vasoactive peptide, endothelin, administered by aerosol in the guinea-pig. Biochem Biophys Res Commun. 1989 Feb 15;158(3):625–632. doi: 10.1016/0006-291x(89)92767-8. [DOI] [PubMed] [Google Scholar]
  25. Lulich K. M., Paterson J. W. An in vitro study of various drugs on central and peripheral airways of the rat: a comparison with human airways. Br J Pharmacol. 1980 Apr;68(4):633–636. doi: 10.1111/j.1476-5381.1980.tb10854.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. MacCumber M. W., Ross C. A., Glaser B. M., Snyder S. H. Endothelin: visualization of mRNAs by in situ hybridization provides evidence for local action. Proc Natl Acad Sci U S A. 1989 Sep;86(18):7285–7289. doi: 10.1073/pnas.86.18.7285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Macquin-Mavier I., Levame M., Istin N., Harf A. Mechanisms of endothelin-mediated bronchoconstriction in the guinea pig. J Pharmacol Exp Ther. 1989 Aug;250(2):740–745. [PubMed] [Google Scholar]
  28. Madison J. M., Brown J. K. Differential inhibitory effects of forskolin, isoproterenol, and dibutyryl cyclic adenosine monophosphate on phosphoinositide hydrolysis in canine tracheal smooth muscle. J Clin Invest. 1988 Oct;82(4):1462–1465. doi: 10.1172/JCI113752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Maggi C. A., Patacchini R., Giuliani S., Meli A. Potent contractile effect of endothelin in isolated guinea-pig airways. Eur J Pharmacol. 1989 Jan 24;160(1):179–182. doi: 10.1016/0014-2999(89)90670-5. [DOI] [PubMed] [Google Scholar]
  30. Marsden P. A., Danthuluri N. R., Brenner B. M., Ballermann B. J., Brock T. A. Endothelin action on vascular smooth muscle involves inositol trisphosphate and calcium mobilization. Biochem Biophys Res Commun. 1989 Jan 16;158(1):86–93. doi: 10.1016/s0006-291x(89)80180-9. [DOI] [PubMed] [Google Scholar]
  31. McCann J. D., Bhalla R. C., Welsh M. J. Release of intracellular calcium by two different second messengers in airway epithelium. Am J Physiol. 1989 Aug;257(2 Pt 1):L116–L124. doi: 10.1152/ajplung.1989.257.2.L116. [DOI] [PubMed] [Google Scholar]
  32. McDonald D. M. Neurogenic inflammation in the rat trachea. I. Changes in venules, leucocytes and epithelial cells. J Neurocytol. 1988 Oct;17(5):583–603. doi: 10.1007/BF01260988. [DOI] [PubMed] [Google Scholar]
  33. McKay K. O., Black J. L., Armour C. L. The mechanism of action of endothelin in human lung. Br J Pharmacol. 1991 Feb;102(2):422–428. doi: 10.1111/j.1476-5381.1991.tb12189.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Meurs H., Roffel A. F., Postema J. B., Timmermans A., Elzinga C. R., Kauffman H. F., Zaagsma J. Evidence for a direct relationship between phosphoinositide metabolism and airway smooth muscle contraction induced by muscarinic agonists. Eur J Pharmacol. 1988 Nov 1;156(2):271–274. doi: 10.1016/0014-2999(88)90331-7. [DOI] [PubMed] [Google Scholar]
  35. Mong S., Miller J., Wu H. L., Crooke S. T. Leukotriene D4 receptor-mediated hydrolysis of phosphoinositide and mobilization of calcium in sheep tracheal smooth muscle cells. J Pharmacol Exp Ther. 1988 Feb;244(2):508–515. [PubMed] [Google Scholar]
  36. Pang D. C., Johns A., Patterson K., Botelho L. H., Rubanyi G. M. Endothelin-1 stimulates phosphatidylinositol hydrolysis and calcium uptake in isolated canine coronary arteries. J Cardiovasc Pharmacol. 1989;13 (Suppl 5):S75–S84. doi: 10.1097/00005344-198900135-00018. [DOI] [PubMed] [Google Scholar]
  37. Payne A. N., Whittle B. J. Potent cyclo-oxygenase-mediated bronchoconstrictor effects of endothelin in the guinea-pig in vivo. Eur J Pharmacol. 1988 Dec 13;158(3):303–304. doi: 10.1016/0014-2999(88)90085-4. [DOI] [PubMed] [Google Scholar]
  38. Pons F., Touvay C., Lagente V., Mencia-Huerta J. M., Braquet P. Comparison of the effects of intra-arterial and aerosol administration of endothelin-1 (ET-1) in the guinea-pig isolated lung. Br J Pharmacol. 1991 Apr;102(4):791–796. doi: 10.1111/j.1476-5381.1991.tb12253.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Rozengurt N., Springall D. R., Polak J. M. Localization of endothelin-like immunoreactivity in airway epithelium of rats and mice. J Pathol. 1990 Jan;160(1):5–8. doi: 10.1002/path.1711600104. [DOI] [PubMed] [Google Scholar]
  40. Takuwa Y., Takuwa N., Rasmussen H. Carbachol induces a rapid and sustained hydrolysis of polyphosphoinositide in bovine tracheal smooth muscle measurements of the mass of polyphosphoinositides, 1,2-diacylglycerol, and phosphatidic acid. J Biol Chem. 1986 Nov 5;261(31):14670–14675. [PubMed] [Google Scholar]
  41. Turner N. C., Power R. F., Polak J. M., Bloom S. R., Dollery C. T. Endothelin-induced contractions of tracheal smooth muscle and identification of specific endothelin binding sites in the trachea of the rat. Br J Pharmacol. 1989 Oct;98(2):361–366. doi: 10.1111/j.1476-5381.1989.tb12605.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Twort C. H., van Breemen C. Human airway smooth muscle in cell culture: control of the intracellular calcium store. Pulm Pharmacol. 1989;2(1):45–53. doi: 10.1016/s0952-0600(89)80009-2. [DOI] [PubMed] [Google Scholar]
  43. Uchida Y., Ninomiya H., Saotome M., Nomura A., Ohtsuka M., Yanagisawa M., Goto K., Masaki T., Hasegawa S. Endothelin, a novel vasoconstrictor peptide, as potent bronchoconstrictor. Eur J Pharmacol. 1988 Sep 13;154(2):227–228. doi: 10.1016/0014-2999(88)90106-9. [DOI] [PubMed] [Google Scholar]
  44. Van Amsterdam R. G., Meurs H., Brouwer F., Postema J. B., Timmermans A., Zaagsma J. Role of phosphoinositide metabolism in functional antagonism of airway smooth muscle contraction by beta-adrenoceptor agonists. Eur J Pharmacol. 1989 May 11;172(2):175–183. doi: 10.1016/0922-4106(89)90008-4. [DOI] [PubMed] [Google Scholar]
  45. Wallenstein S., Zucker C. L., Fleiss J. L. Some statistical methods useful in circulation research. Circ Res. 1980 Jul;47(1):1–9. doi: 10.1161/01.res.47.1.1. [DOI] [PubMed] [Google Scholar]
  46. Weichman B. M., Muccitelli R. M., Osborn R. R., Holden D. A., Gleason J. G., Wasserman M. A. In vitro and in vivo mechanisms of leukotriene-mediated bronchoconstriction in the guinea pig. J Pharmacol Exp Ther. 1982 Jul;222(1):202–208. [PubMed] [Google Scholar]
  47. Xuan Y. T., Whorton A. R., Watkins W. D. Inhibition by nicardipine of endothelin-mediated inositol phosphate formation and Ca2+ mobilization in smooth muscle cell. Biochem Biophys Res Commun. 1989 Apr 28;160(2):758–764. doi: 10.1016/0006-291x(89)92498-4. [DOI] [PubMed] [Google Scholar]
  48. Yanagisawa M., Kurihara H., Kimura S., Tomobe Y., Kobayashi M., Mitsui Y., Yazaki Y., Goto K., Masaki T. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature. 1988 Mar 31;332(6163):411–415. doi: 10.1038/332411a0. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES