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. 1994 Feb;111(2):431–434. doi: 10.1111/j.1476-5381.1994.tb14753.x

Induction of emesis in Suncus murinus by pyrogallol, a generator of free radicals.

Y Torii 1, H Saito 1, N Matsuki 1
PMCID: PMC1909966  PMID: 8004387

Abstract

1. We investigated whether or not pyrogallol, a generator of free radicals, is emetogenic in Suncus murinus, the house musk shrew. Pyrogallol (i.p.) caused dose-dependent emesis in suncus with an ED50 value of 77.3 mg kg-1. At a dose of 128 mg kg-1, all suncus vomited with mean latency of 18.8 +/- 5.2 min and the number of vomiting episodes was 8.6 +/- 2.9. 2. The prophylactic effects of N-(2-mercaptopropionyl)-glycine (MPG), an antioxidant, and tropisetron, a 5-hydroxytryptamine3 (5-HT3) receptor antagonist, were studied. Pyrogallol (128 mg kg-1, i.p.)-induced emesis was prevented by treatment with MPG (i.p.) or tropisetron (s.c.) with ID50 values of 149 mg kg-1 and 117 micrograms kg-1, respectively. 3. Pyrogallol-induced emesis was completely prevented by surgical abdominal vagotomy. 4. The present results indicate that pyrogallol-induced emesis is characteristically very similar to that caused by cisplatin and support the idea that generation of free radicals causes the release of peripheral 5-HT, which stimulates vagal afferent sensory nerves to cause emesis.

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Selected References

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  1. AXELROD J., TOMCHICK R. Enzymatic O-methylation of epinephrine and other catechols. J Biol Chem. 1958 Sep;233(3):702–705. [PubMed] [Google Scholar]
  2. Andrews P. L., Rapeport W. G., Sanger G. J. Neuropharmacology of emesis induced by anti-cancer therapy. Trends Pharmacol Sci. 1988 Sep;9(9):334–341. doi: 10.1016/0165-6147(88)90106-x. [DOI] [PubMed] [Google Scholar]
  3. Baldessarini R. J., Greiner E. Inhibition of catechol-O-methyl transferase by catechols and polyphenols. Biochem Pharmacol. 1973 Jan 15;22(2):247–256. doi: 10.1016/0006-2952(73)90277-3. [DOI] [PubMed] [Google Scholar]
  4. Beleslin D. B., Strbac M. Noradrenaline-induced emesis. Alpha-2 adrenoceptor mediation in the area postrema. Neuropharmacology. 1987 Aug;26(8):1157–1165. doi: 10.1016/0028-3908(87)90262-0. [DOI] [PubMed] [Google Scholar]
  5. CROUT J. R. Inhibition of catechol-O-methyl transferase by pyrogallol in the rat. Biochem Pharmacol. 1961 Apr;6:47–50. doi: 10.1016/0006-2952(61)90067-3. [DOI] [PubMed] [Google Scholar]
  6. Dobyan D. C., Bull J. M., Strebel F. R., Sunderland B. A., Bulger R. E. Protective effects of O-(beta-hydroxyethyl)-rutoside on cis-platinum-induced acute renal failure in the rat. Lab Invest. 1986 Nov;55(5):557–563. [PubMed] [Google Scholar]
  7. Eccleston D., Ritchie I. M. Sulphate ester formation from catecholamine metabolites and pyrogallol in rat brain in vivo. J Neurochem. 1973 Sep;21(3):635–646. doi: 10.1111/j.1471-4159.1973.tb06008.x. [DOI] [PubMed] [Google Scholar]
  8. Goldschmidt J. E., Tallarida R. J. Pharmacological evidence that captopril possesses an endothelium-mediated component of vasodilation: effect of sulfhydryl groups on endothelium-derived relaxing factor. J Pharmacol Exp Ther. 1991 Jun;257(3):1136–1145. [PubMed] [Google Scholar]
  9. Hannemann J., Baumann K. Cisplatin-induced lipid peroxidation and decrease of gluconeogenesis in rat kidney cortex: different effects of antioxidants and radical scavengers. Toxicology. 1988 Oct;51(2-3):119–132. doi: 10.1016/0300-483x(88)90143-6. [DOI] [PubMed] [Google Scholar]
  10. Hollingshead L. M., Goa K. L. Recombinant granulocyte colony-stimulating factor (rG-CSF). A review of its pharmacological properties and prospective role in neutropenic conditions. Drugs. 1991 Aug;42(2):300–330. doi: 10.2165/00003495-199142020-00009. [DOI] [PubMed] [Google Scholar]
  11. Lundberg J., Ahlman H., Dahlström A., Kewenter J. Catecholamine-containing nerve fibres in the human abdominal vagus. Gastroenterology. 1976 Mar;70(3):472–474. [PubMed] [Google Scholar]
  12. Marklund S., Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974 Sep 16;47(3):469–474. doi: 10.1111/j.1432-1033.1974.tb03714.x. [DOI] [PubMed] [Google Scholar]
  13. McGinness J. E., Proctor P. H., Demopoulos H. B., Hokanson J. A., Kirkpatrick D. S. Amelioration of cis-platinum nephrotoxicity by orgotein (superoxide dismutase). Physiol Chem Phys. 1978;10(3):267–277. [PubMed] [Google Scholar]
  14. Mutoh M., Imanishi H., Torii Y., Tamura M., Saito H., Matsuki N. Cisplatin-induced emesis in Suncus murinus. Jpn J Pharmacol. 1992 Mar;58(3):321–324. doi: 10.1254/jjp.58.321. [DOI] [PubMed] [Google Scholar]
  15. Oku M., Noda T., Kiyozuka Y., Ninomiya Y., Hino K., Okamura Y., Maruyama M., Ichijo M. [Pharmacokinetic analysis of intraperitoneal cisplatinum administration]. Nihon Gan Chiryo Gakkai Shi. 1988 Mar 20;23(3):657–664. [PubMed] [Google Scholar]
  16. Racké K., Schwörer H. Regulation of serotonin release from the intestinal mucosa. Pharmacol Res. 1991 Jan;23(1):13–25. doi: 10.1016/s1043-6618(05)80101-x. [DOI] [PubMed] [Google Scholar]
  17. Rozencweig M., von Hoff D. D., Slavik M., Muggia F. M. Cis-diamminedichloroplatinum (II). A new anticancer drug. Ann Intern Med. 1977 Jun;86(6):803–812. doi: 10.7326/0003-4819-86-6-803. [DOI] [PubMed] [Google Scholar]
  18. Sangeetha P., Das U. N., Koratkar R., Suryaprabha P. Increase in free radical generation and lipid peroxidation following chemotherapy in patients with cancer. Free Radic Biol Med. 1990;8(1):15–19. doi: 10.1016/0891-5849(90)90139-a. [DOI] [PubMed] [Google Scholar]
  19. Sugihara K., Gemba M. Modification of cisplatin toxicity by antioxidants. Jpn J Pharmacol. 1986 Feb;40(2):353–355. doi: 10.1254/jjp.40.353. [DOI] [PubMed] [Google Scholar]
  20. Torii Y., Mutoh M., Saito H., Matsuki N. Involvement of free radicals in cisplatin-induced emesis in Suncus murinus. Eur J Pharmacol. 1993 Aug 2;248(2):131–135. doi: 10.1016/0926-6917(93)90034-n. [DOI] [PubMed] [Google Scholar]
  21. Torii Y., Saito H., Matsuki N. 5-Hydroxytryptamine is emetogenic in the house musk shrew, Suncus murinus. Naunyn Schmiedebergs Arch Pharmacol. 1991 Nov;344(5):564–567. doi: 10.1007/BF00170653. [DOI] [PubMed] [Google Scholar]
  22. Torii Y., Saito H., Matsuki N. Selective blockade of cytotoxic drug-induced emesis by 5-HT3 receptor antagonists in Suncus murinus. Jpn J Pharmacol. 1991 Jan;55(1):107–113. doi: 10.1254/jjp.55.107. [DOI] [PubMed] [Google Scholar]
  23. Torii Y., Shikita M., Saito H., Matsuki N. X-irradiation-induced emesis in Suncus murinus. J Radiat Res. 1993 Jun;34(2):164–170. doi: 10.1269/jrr.34.164. [DOI] [PubMed] [Google Scholar]

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