Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1975 Apr;79(1):147–169.

An experimental mouse testicular teratoma as a model for neuroepithelial neoplasia and differentiation. I. Light microscopic and tissue and organ culture observations.

S R VandenBerg, M M Herman, S K Ludwin, A Bignami
PMCID: PMC1912641  PMID: 164776

Abstract

The various stages of divergent neuroepithelial differentiation were studied in the solid transplants of a transplantable mouse testicular teratoma (OTT-6050) maintained in both ascitic and solid forms. They included: a) areas of undifferentiated medullary epithelium corresponding to the rare human medulloepithelioma; b) areas of neuroblastic differentiation corresponding to neuroblastoma, with more mature neuronal differentiation corresponding to ganglioneuroma or, when mixed with glial elements, to ganglioglioma; and c) more mature neuroglial areas resembling astrocytoma, oligodendroglioma or ependymoma, as well as more primitive areas corresponding to ependymoblastoma. In tissue culture using collagen-coated coverslips, astrocytic differentiation was found in the outgrowth zone after 15 days, confirmed by immunofluorescence with antibodies to an astroglia-specific protein. In organ culture systems, glial components, including ependymal structures, were preserved in tumor explants, and astrocytic differentiation, as expressed by glial fiber formation, was increased after 4 to 6 weeks in vitro. No neuronal differentiation was demonstrable, however. The neuroepithelial component of this experimental teratoma may provide a model for the study of neoplastic neuroepithelial differentiation.

Full text

PDF
151

Images in this article

166Fig 9
on p.166
166Fig 10
on p.166
166Fig 11
on p.166
166Fig 12
on p.166
163Fig 1
on p.163
163Fig 2
on p.163
163Fig 3
on p.163
163Fig 4
on p.163
167Fig 13
on p.167
167Fig 14
on p.167
167Fig 15
on p.167
168Fig 16
on p.168
168Fig 17
on p.168
168Fig 18
on p.168
165Fig 5
on p.165
165Fig 6
on p.165
165Fig 7
on p.165
165Fig 8
on p.165

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Artzt K., Bennett D. A genetically caused embryonal ectodermal tumor in the mouse. J Natl Cancer Inst. 1972 Jan;48(1):141–158. [PubMed] [Google Scholar]
  2. Bignami A., Dahl D. Astrocyte-specific protein and neuroglial differentiation. An immunofluorescence study with antibodies to the glial fibrillary acidic protein. J Comp Neurol. 1974 Jan 1;153(1):27–38. doi: 10.1002/cne.901530104. [DOI] [PubMed] [Google Scholar]
  3. Bignami A., Eng L. F., Dahl D., Uyeda C. T. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 1972 Aug 25;43(2):429–435. doi: 10.1016/0006-8993(72)90398-8. [DOI] [PubMed] [Google Scholar]
  4. Damjanov I., Solter D., Belicza M., Skreb N. Teratomas obtained through extrauterine growth of seven-day mouse embryos. J Natl Cancer Inst. 1971 Mar;46(3):471–passim. [PubMed] [Google Scholar]
  5. Damjanov I., Solter D., Serman D. Teratocarcinoma with the capacity for differentiation restricted to neuro-ectodermal tissue. Virchows Arch B Cell Pathol. 1973 Jul 22;13(3):179–195. doi: 10.1007/BF02889307. [DOI] [PubMed] [Google Scholar]
  6. Deck J. H. Cerebral medulloepithelioma with maturation into ependymal cells and ganglion cells. J Neuropathol Exp Neurol. 1969 Jul;28(3):442–454. doi: 10.1097/00005072-196907000-00006. [DOI] [PubMed] [Google Scholar]
  7. Eng L. F., Vanderhaeghen J. J., Bignami A., Gerstl B. An acidic protein isolated from fibrous astrocytes. Brain Res. 1971 May 7;28(2):351–354. doi: 10.1016/0006-8993(71)90668-8. [DOI] [PubMed] [Google Scholar]
  8. Evans M. J. The isolation and properties of a clonal tissue culture strain of pluripotent mouse teratoma cells. J Embryol Exp Morphol. 1972 Aug;28(1):163–176. [PubMed] [Google Scholar]
  9. FAWCETT D. W. Bilateral ovarian teratomas in a mouse. Cancer Res. 1950 Nov;10(11):705–707. [PubMed] [Google Scholar]
  10. FEKETE E., FERRIGNO M. A. Studies on a transplantable teratoma of the mouse. Cancer Res. 1952 Jun;12(6):438–440. [PubMed] [Google Scholar]
  11. Jami J., Ritz E. Multipotentiality of single cells of transplantable teratocarcinomas derived from mouse embryo grafts. J Natl Cancer Inst. 1974 May;52(5):1547–1552. doi: 10.1093/jnci/52.5.1547. [DOI] [PubMed] [Google Scholar]
  12. Jellinger K. Cerebral medulloepithelioma. Acta Neuropathol. 1972;22(2):95–101. doi: 10.1007/BF00688777. [DOI] [PubMed] [Google Scholar]
  13. KLEINSMITH L. J., PIERCE G. B., Jr MULTIPOTENTIALITY OF SINGLE EMBRYONAL CARCINOMA CELLS. Cancer Res. 1964 Oct;24:1544–1551. [PubMed] [Google Scholar]
  14. Kahan B. W., Ephrussi B. Developmental potentialities of clonal in vitro cultures of mouse testicular teratoma. J Natl Cancer Inst. 1970 May;44(5):1015–1036. [PubMed] [Google Scholar]
  15. Karch S. B., Urich H. Medulloepithelioma: definition of an entity. J Neuropathol Exp Neurol. 1972 Jan;31(1):27–53. [PubMed] [Google Scholar]
  16. LAPHAM L. W., JOHNSTONE M. A., BRUNDJAR K. H. A NEW PARAFFIN METHOD FOR THE COMBINED STAINING OF MYELIN AND GLIAL FIBERS. J Neuropathol Exp Neurol. 1964 Jan;23:156–160. [PubMed] [Google Scholar]
  17. LEIGHTON J. The growth patterns of some transplantable animal tumors in sponge matrix tissue culture. J Natl Cancer Inst. 1954 Oct;15(2):275–293. [PubMed] [Google Scholar]
  18. Lehman J. M., Speers W. C., Swartzendruber D. E., Pierce G. B. Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma. J Cell Physiol. 1974 Aug;84(1):13–27. doi: 10.1002/jcp.1040840103. [DOI] [PubMed] [Google Scholar]
  19. Levine A. J., Torosian M., Sarokhan A. J., Teresky A. K. Biochemical criteria for the in vitro differentiation of embryoid bodies produced by a transplantable teratoma of mice. The production of acetylcholine esterase and creatine phosphokinase by teratoma cells. J Cell Physiol. 1974 Oct;84(2):311–317. doi: 10.1002/jcp.1040840217. [DOI] [PubMed] [Google Scholar]
  20. Meier H., Myers D. D., Fox R. R., Laird C. W. Occurrence, pathological features, and propagation of gonadal teratomas in inbred mice and in rabbits. Cancer Res. 1970 Jan;30(1):30–34. [PubMed] [Google Scholar]
  21. Mukai N., Kobayashi S. Human adenovirus-induced medulloepitheliomatous neoplasms in Sprague-Dawley rats. Am J Pathol. 1973 Dec;73(3):671–690. [PMC free article] [PubMed] [Google Scholar]
  22. Mukai N., Kobayashi S. Primary brain and spinal cord tumors induced by human adenovirus type 12 in hamsters. J Neuropathol Exp Neurol. 1973 Oct;32(4):523–541. doi: 10.1097/00005072-197310000-00004. [DOI] [PubMed] [Google Scholar]
  23. PIERCE G. B., DIXON F. J., Jr Testicular teratomas. I. Demonstration of teratogenesis by metamorphosis of multipotential cells. Cancer. 1959 May-Jun;12(3):573–583. doi: 10.1002/1097-0142(195905/06)12:3<573::aid-cncr2820120316>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  24. PIERCE G. B., Jr, DIXON F. J., Jr, VERNEY E. L. Teratocarcinogenic and tissue-forming potentials of the cell types comprising neoplastic embryoid bodies. Lab Invest. 1960 Nov-Dec;9:583–602. [PubMed] [Google Scholar]
  25. PIERCE G. B., Jr, VERNEY E. L. An in vitro and in vivo study of differentiation in teratocarcinomas. Cancer. 1961 Sep-Oct;14:1017–1029. doi: 10.1002/1097-0142(196109/10)14:5<1017::aid-cncr2820140516>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
  26. Rosenthal M. D., Wishnow R. M., Sato G. H. In vitro growth and differetiation of clonal populations of multipotential mouse clls derived from a transplantable testicular teratocarcinoma. J Natl Cancer Inst. 1970 May;44(5):1001–1014. [PubMed] [Google Scholar]
  27. Rubinstein L. J., Herman M. M., Foley V. L. In vitro characteristics of human glioblastomas maintained in organ culture systems. Light microscopy observations. Am J Pathol. 1973 Apr;71(1):61–80. [PMC free article] [PubMed] [Google Scholar]
  28. Rubinstein L. J. Presidential address. Cytogenesis and differentiation of primitive central neuroepithelial tumors. J Neuropathol Exp Neurol. 1972 Jan;31(1):7–26. doi: 10.1097/00005072-197201000-00002. [DOI] [PubMed] [Google Scholar]
  29. Rubinstein L. J. The definition of the ependymoblastoma. Arch Pathol. 1970 Jul;90(1):35–45. [PubMed] [Google Scholar]
  30. STEVENS L. C. EXPERIMENTAL PRODUCTION OF TESTICULAR TERATOMAS IN MICE. Proc Natl Acad Sci U S A. 1964 Sep;52:654–661. doi: 10.1073/pnas.52.3.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. STEVENS L. C. Embryology of testicular teratomas in strain 129 mice. J Natl Cancer Inst. 1959 Dec;23:1249–1295. [PubMed] [Google Scholar]
  32. STEVENS L. C. Embryonic potency of embryoid bodies derived from a transplantable testicular teratoma of the mouse. Dev Biol. 1960 Jun;2:285–297. doi: 10.1016/0012-1606(60)90010-5. [DOI] [PubMed] [Google Scholar]
  33. STEVENS L. C., HUMMEL K. P. A description of spontaneous congenital testicular teratomas in strain 129 mice. J Natl Cancer Inst. 1957 May;18(5):719–747. [PubMed] [Google Scholar]
  34. STEVENS L. C. Studies on transplantable testicular teratomas of strain 129 mice. J Natl Cancer Inst. 1958 Jun;20(6):1257–1275. doi: 10.1093/jnci/20.6.1257. [DOI] [PubMed] [Google Scholar]
  35. Sipe J. C., Herman M. M., Rubinstein L. J. Electron microscopic observations on human glioblastomas and astrocytomas maintained in organ culture systems. Am J Pathol. 1973 Dec;73(3):589–606. [PMC free article] [PubMed] [Google Scholar]
  36. Sipe J. C., Rubinstein L. J., Herman M. M., Bignami A. Ethylnitrosourea-induced astrocytomas. Morphologic observations on rat tumors maintained in tissue and organ culture systems. Lab Invest. 1974 Dec;31(6):571–579. [PubMed] [Google Scholar]
  37. Stevens L. C. A new inbred subline of mice (129-terSv) with a high incidence of spontaneous congenital testicular teratomas. J Natl Cancer Inst. 1973 Jan;50(1):235–242. doi: 10.1093/jnci/50.1.235. [DOI] [PubMed] [Google Scholar]
  38. Stevens L. C. The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev Biol. 1970 Mar;21(3):364–382. doi: 10.1016/0012-1606(70)90130-2. [DOI] [PubMed] [Google Scholar]
  39. Stevens L. C., Varnum D. S. The development of teratomas from parthenogenetically activated ovarian mouse eggs. Dev Biol. 1974 Apr;37(2):369–380. doi: 10.1016/0012-1606(74)90155-9. [DOI] [PubMed] [Google Scholar]
  40. THIERY M. OVARIAN TERATOMA IN THE MOUSE. Br J Cancer. 1963 Jun;17:231–234. doi: 10.1038/bjc.1963.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Teresky A. K., Marsden M., Kuff E. L., Levine A. J. Morphological criteria for the in vitro differentiation of embryoid bodies produced by a transplantable teratoma of mice. J Cell Physiol. 1974 Oct;84(2):319–332. doi: 10.1002/jcp.1040840218. [DOI] [PubMed] [Google Scholar]
  42. Uyeda C. T., Eng L. F., Bignami A. Immunological study of the glial fibrillary acidic protein. Brain Res. 1972 Feb 11;37(1):81–89. doi: 10.1016/0006-8993(72)90347-2. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES