Skip to main content
PMC home page
American Journal of Human Genetics logoLink to American Journal of Human Genetics
. 1996 Jun;58(6):1260–1267.

Deletion mapping of gliomas suggest the presence of two small regions for candidate tumor-suppressor genes in a 17-cM interval on chromosome 10q.

R Albarosa 1, B M Colombo 1, L Roz 1, I Magnani 1, B Pollo 1, N Cirenei 1, C Giani 1, A M Conti 1, S DiDonato 1, G Finocchiaro 1
PMCID: PMC1915052  PMID: 8651304

Abstract

The loss of genetic material on chromosome 10q is frequent in different tumors and particularly in malignant gliomas. We analyzed 90 of these tumors and found loss of heterozygosity (LOH) in >90% of the informative loci in glioblastoma multiforme (GBM). Initial studies restricted the common LOH region to 10q24-qter. Subsequently, the study of a pediatric GBM suggested D10S221 and D10S209, respectively, as centromeric and telomeric markers of a 4-cM LOH region. It is interesting to note that, in one subset of cells from this tumor, locus D10S209 seems involved in the allelic imbalance of a larger region, with D10S214 as telomeric marker. This 17-cM region contains the D10S587-D10S216 interval of common deletion recently defined on another set of gliomas.

Full text

PDF
1264

Images in this article

1263Figure 2
on p.1263
1262Figure 1
on p.1262
1265Figure 4
on p.1265
1265Figure 5
on p.1265

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albarosa R., DiDonato S., Finocchiaro G. Redefinition of the coding sequence of the MXI1 gene and identification of a polymorphic repeat in the 3' non-coding region that allows the detection of loss of heterozygosity of chromosome 10q25 in glioblastomas. Hum Genet. 1995 Jun;95(6):709–711. doi: 10.1007/BF00209493. [DOI] [PubMed] [Google Scholar]
  2. Chumakov I. M., Rigault P., Le Gall I., Bellanné-Chantelot C., Billault A., Guillou S., Soularue P., Guasconi G., Poullier E., Gros I. A YAC contig map of the human genome. Nature. 1995 Sep 28;377(6547 Suppl):175–297. doi: 10.1038/377175a0. [DOI] [PubMed] [Google Scholar]
  3. Costello J. F., Futscher B. W., Kroes R. A., Pieper R. O. Methylation-related chromatin structure is associated with exclusion of transcription factors from and suppressed expression of the O-6-methylguanine DNA methyltransferase gene in human glioma cell lines. Mol Cell Biol. 1994 Oct;14(10):6515–6521. doi: 10.1128/mcb.14.10.6515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Decker R. A., Moore J., Ponder B., Weber J. L. Linkage mapping of human chromosome 10 microsatellite polymorphisms. Genomics. 1992 Mar;12(3):604–606. doi: 10.1016/0888-7543(92)90455-2. [DOI] [PubMed] [Google Scholar]
  5. Eagle L. R., Yin X., Brothman A. R., Williams B. J., Atkin N. B., Prochownik E. V. Mutation of the MXI1 gene in prostate cancer. Nat Genet. 1995 Mar;9(3):249–255. doi: 10.1038/ng0395-249. [DOI] [PubMed] [Google Scholar]
  6. Edelhoff S., Ayer D. E., Zervos A. S., Steingrímsson E., Jenkins N. A., Copeland N. G., Eisenman R. N., Brent R., Disteche C. M. Mapping of two genes encoding members of a distinct subfamily of MAX interacting proteins: MAD to human chromosome 2 and mouse chromosome 6, and MXI1 to human chromosome 10 and mouse chromosome 19. Oncogene. 1994 Feb;9(2):665–668. [PubMed] [Google Scholar]
  7. Fults D., Pedone C. Deletion mapping of the long arm of chromosome 10 in glioblastoma multiforme. Genes Chromosomes Cancer. 1993 Jul;7(3):173–177. doi: 10.1002/gcc.2870070311. [DOI] [PubMed] [Google Scholar]
  8. Gardner E., Rydberg B., Karran P., Ponder B. A. Localization of the human O6-methylguanine-DNA methyltransferase gene to chromosome 10q24.33-qter. Genomics. 1991 Oct;11(2):475–476. doi: 10.1016/0888-7543(91)90162-8. [DOI] [PubMed] [Google Scholar]
  9. Gray I. C., Phillips S. M., Lee S. J., Neoptolemos J. P., Weissenbach J., Spurr N. K. Loss of the chromosomal region 10q23-25 in prostate cancer. Cancer Res. 1995 Nov 1;55(21):4800–4803. [PubMed] [Google Scholar]
  10. Gyapay G., Morissette J., Vignal A., Dib C., Fizames C., Millasseau P., Marc S., Bernardi G., Lathrop M., Weissenbach J. The 1993-94 Généthon human genetic linkage map. Nat Genet. 1994 Jun;7(2 Spec No):246–339. doi: 10.1038/ng0694supp-246. [DOI] [PubMed] [Google Scholar]
  11. Herbst R. A., Weiss J., Ehnis A., Cavenee W. K., Arden K. C. Loss of heterozygosity for 10q22-10qter in malignant melanoma progression. Cancer Res. 1994 Jun 15;54(12):3111–3114. [PubMed] [Google Scholar]
  12. Karlbom A. E., James C. D., Boethius J., Cavenee W. K., Collins V. P., Nordenskjöld M., Larsson C. Loss of heterozygosity in malignant gliomas involves at least three distinct regions on chromosome 10. Hum Genet. 1993 Sep;92(2):169–174. doi: 10.1007/BF00219686. [DOI] [PubMed] [Google Scholar]
  13. Lang F. F., Miller D. C., Koslow M., Newcomb E. W. Pathways leading to glioblastoma multiforme: a molecular analysis of genetic alterations in 65 astrocytic tumors. J Neurosurg. 1994 Sep;81(3):427–436. doi: 10.3171/jns.1994.81.3.0427. [DOI] [PubMed] [Google Scholar]
  14. Magnani I., Guerneri S., Pollo B., Cirenei N., Colombo B. M., Broggi G., Galli C., Bugiani O., DiDonato S., Finocchiaro G. Increasing complexity of the karyotype in 50 human gliomas. Progressive evolution and de novo occurrence of cytogenetic alterations. Cancer Genet Cytogenet. 1994 Jul 15;75(2):77–89. doi: 10.1016/0165-4608(94)90157-0. [DOI] [PubMed] [Google Scholar]
  15. Matise T. C., Perlin M., Chakravarti A. Automated construction of genetic linkage maps using an expert system (MultiMap): a human genome linkage map. Nat Genet. 1994 Apr;6(4):384–390. doi: 10.1038/ng0494-384. [DOI] [PubMed] [Google Scholar]
  16. Mineura K., Izumi I., Kuwahara N., Kowada M. O6-methylguanine-DNA methyltransferase activity in cerebral gliomas. A guidance for nitrosourea treatment? Acta Oncol. 1994;33(1):29–32. doi: 10.3109/02841869409098371. [DOI] [PubMed] [Google Scholar]
  17. Morita R., Saito S., Ishikawa J., Ogawa O., Yoshida O., Yamakawa K., Nakamura Y. Common regions of deletion on chromosomes 5q, 6q, and 10q in renal cell carcinoma. Cancer Res. 1991 Nov 1;51(21):5817–5820. [PubMed] [Google Scholar]
  18. Pegg A. E. Mammalian O6-alkylguanine-DNA alkyltransferase: regulation and importance in response to alkylating carcinogenic and therapeutic agents. Cancer Res. 1990 Oct 1;50(19):6119–6129. [PubMed] [Google Scholar]
  19. Peiffer S. L., Herzog T. J., Tribune D. J., Mutch D. G., Gersell D. J., Goodfellow P. J. Allelic loss of sequences from the long arm of chromosome 10 and replication errors in endometrial cancers. Cancer Res. 1995 May 1;55(9):1922–1926. [PubMed] [Google Scholar]
  20. Pershouse M. A., Stubblefield E., Hadi A., Killary A. M., Yung W. K., Steck P. A. Analysis of the functional role of chromosome 10 loss in human glioblastomas. Cancer Res. 1993 Oct 15;53(20):5043–5050. [PubMed] [Google Scholar]
  21. Rasheed B. K., Fuller G. N., Friedman A. H., Bigner D. D., Bigner S. H. Loss of heterozygosity for 10q loci in human gliomas. Genes Chromosomes Cancer. 1992 Jul;5(1):75–82. doi: 10.1002/gcc.2870050111. [DOI] [PubMed] [Google Scholar]
  22. Rasheed B. K., McLendon R. E., Friedman H. S., Friedman A. H., Fuchs H. E., Bigner D. D., Bigner S. H. Chromosome 10 deletion mapping in human gliomas: a common deletion region in 10q25. Oncogene. 1995 Jun 1;10(11):2243–2246. [PubMed] [Google Scholar]
  23. Rempel S. A., Schwechheimer K., Davis R. L., Cavenee W. K., Rosenblum M. L. Loss of heterozygosity for loci on chromosome 10 is associated with morphologically malignant meningioma progression. Cancer Res. 1993 May 15;53(10 Suppl):2386–2392. [PubMed] [Google Scholar]
  24. Shindo H., Tani E., Matsumuto T., Hashimoto T., Furuyama J. Stabilization of c-myc protein in human glioma cells. Acta Neuropathol. 1993;86(4):345–352. doi: 10.1007/BF00369446. [DOI] [PubMed] [Google Scholar]
  25. Speaks S. L., Sanger W. G., Masih A. S., Harrington D. S., Hess M., Armitage J. O. Recurrent abnormalities of chromosome bands 10q23-25 in non-Hodgkin's lymphoma. Genes Chromosomes Cancer. 1992 Oct;5(3):239–243. doi: 10.1002/gcc.2870050311. [DOI] [PubMed] [Google Scholar]
  26. Tenan M., Colombo B. M., Pollo B., Cajola L., Broggi G., Finocchiaro G. p53 mutations and microsatellite analysis of loss of heterozygosity in malignant gliomas. Cancer Genet Cytogenet. 1994 Jun;74(2):139–143. doi: 10.1016/0165-4608(94)90012-4. [DOI] [PubMed] [Google Scholar]
  27. Voullaire L. E., Slater H. R., Petrovic V., Choo K. H. A functional marker centromere with no detectable alpha-satellite, satellite III, or CENP-B protein: activation of a latent centromere? Am J Hum Genet. 1993 Jun;52(6):1153–1163. [PMC free article] [PubMed] [Google Scholar]
  28. Weissenbach J., Gyapay G., Dib C., Vignal A., Morissette J., Millasseau P., Vaysseix G., Lathrop M. A second-generation linkage map of the human genome. Nature. 1992 Oct 29;359(6398):794–801. doi: 10.1038/359794a0. [DOI] [PubMed] [Google Scholar]
  29. Zervos A. S., Gyuris J., Brent R. Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites. Cell. 1993 Jan 29;72(2):223–232. doi: 10.1016/0092-8674(93)90662-a. [DOI] [PubMed] [Google Scholar]

Articles from American Journal of Human Genetics are provided here courtesy of American Society of Human Genetics

RESOURCES