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. 1997 May;71(5):3986–3991. doi: 10.1128/jvi.71.5.3986-3991.1997

Molecular determinants for virulence in coxsackievirus B1 infection.

J E Rinehart 1, R M Gómez 1, R P Roos 1
PMCID: PMC191550  PMID: 9094675

Abstract

Studies demonstrated that a strain derived from an infectious clone of coxsackievirus B1 (CVB1N) (N. Iizuka, H. Yonekawa, and A. Nomoto, J. Virol. 65:4867-4873, 1991) was 3 to 4 log10 less virulent than the myotropic Tucson strain of CVB1 (CVB1T) following intraperitoneal inoculation of newborn mice. Replacement of nucleotides (nt) 69 to 804 from the 5' untranslated region (5' UTR) and 1A coding region of CVB1N or nt 117 to 161 from the 5' UTR with the corresponding part from CVB1T restored greater than 90% of the virulence. Sequencing of the 5' UTR of CVB1T demonstrated areas with a greater similarity to particular echoviruses than to CVB1N, suggesting that recombination events might have occurred, perhaps influencing the virulence phenotype.

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Selected References

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  1. Auvinen P., Stanway G., Hyypiä T. Genetic diversity of enterovirus subgroups. Arch Virol. 1989;104(3-4):175–186. doi: 10.1007/BF01315541. [DOI] [PubMed] [Google Scholar]
  2. Cammack N., Phillips A., Dunn G., Patel V., Minor P. D. Intertypic genomic rearrangements of poliovirus strains in vaccinees. Virology. 1988 Dec;167(2):507–514. [PubMed] [Google Scholar]
  3. Chapman N. M., Tracy S., Gauntt C. J., Fortmueller U. Molecular detection and identification of enteroviruses using enzymatic amplification and nucleic acid hybridization. J Clin Microbiol. 1990 May;28(5):843–850. doi: 10.1128/jcm.28.5.843-850.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diedrich S., Driesel G., Schreier E. Sequence comparison of echovirus type 30 isolates to other enteroviruses in the 5'noncoding region. J Med Virol. 1995 Jun;46(2):148–152. doi: 10.1002/jmv.1890460212. [DOI] [PubMed] [Google Scholar]
  5. Evans D. M., Dunn G., Minor P. D., Schild G. C., Cann A. J., Stanway G., Almond J. W., Currey K., Maizel J. V., Jr Increased neurovirulence associated with a single nucleotide change in a noncoding region of the Sabin type 3 poliovaccine genome. Nature. 1985 Apr 11;314(6011):548–550. doi: 10.1038/314548a0. [DOI] [PubMed] [Google Scholar]
  6. Furione M., Guillot S., Otelea D., Balanant J., Candrea A., Crainic R. Polioviruses with natural recombinant genomes isolated from vaccine-associated paralytic poliomyelitis. Virology. 1993 Sep;196(1):199–208. doi: 10.1006/viro.1993.1468. [DOI] [PubMed] [Google Scholar]
  7. Georgescu M. M., Delpeyroux F., Crainic R. Tripartite genome organization of a natural type 2 vaccine/nonvaccine recombinant poliovirus. J Gen Virol. 1995 Sep;76(Pt 9):2343–2348. doi: 10.1099/0022-1317-76-9-2343. [DOI] [PubMed] [Google Scholar]
  8. Huttunen P., Santti J., Pulli T., Hyypiä T. The major echovirus group is genetically coherent and related to coxsackie B viruses. J Gen Virol. 1996 Apr;77(Pt 4):715–725. doi: 10.1099/0022-1317-77-4-715. [DOI] [PubMed] [Google Scholar]
  9. Iizuka N., Kuge S., Nomoto A. Complete nucleotide sequence of the genome of coxsackievirus B1. Virology. 1987 Jan;156(1):64–73. doi: 10.1016/0042-6822(87)90436-3. [DOI] [PubMed] [Google Scholar]
  10. Iizuka N., Yonekawa H., Nomoto A. Nucleotide sequences important for translation initiation of enterovirus RNA. J Virol. 1991 Sep;65(9):4867–4873. doi: 10.1128/jvi.65.9.4867-4873.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jackson R. J., Kaminski A. Internal initiation of translation in eukaryotes: the picornavirus paradigm and beyond. RNA. 1995 Dec;1(10):985–1000. [PMC free article] [PubMed] [Google Scholar]
  12. Jakob J., Roos R. P. Molecular determinants of Theiler's murine encephalomyelitis-induced disease. J Neurovirol. 1996 Apr;2(2):70–77. doi: 10.3109/13550289609146540. [DOI] [PubMed] [Google Scholar]
  13. Jang S. K., Pestova T. V., Hellen C. U., Witherell G. W., Wimmer E. Cap-independent translation of picornavirus RNAs: structure and function of the internal ribosomal entry site. Enzyme. 1990;44(1-4):292–309. doi: 10.1159/000468766. [DOI] [PubMed] [Google Scholar]
  14. Kawamura N., Kohara M., Abe S., Komatsu T., Tago K., Arita M., Nomoto A. Determinants in the 5' noncoding region of poliovirus Sabin 1 RNA that influence the attenuation phenotype. J Virol. 1989 Mar;63(3):1302–1309. doi: 10.1128/jvi.63.3.1302-1309.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kirkegaard K., Baltimore D. The mechanism of RNA recombination in poliovirus. Cell. 1986 Nov 7;47(3):433–443. doi: 10.1016/0092-8674(86)90600-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kong W. P., Ghadge G. D., Roos R. P. Involvement of cardiovirus leader in host cell-restricted virus expression. Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1796–1800. doi: 10.1073/pnas.91.5.1796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. La Monica N., Meriam C., Racaniello V. R. Mapping of sequences required for mouse neurovirulence of poliovirus type 2 Lansing. J Virol. 1986 Feb;57(2):515–525. doi: 10.1128/jvi.57.2.515-525.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Minnich L. L., Ray C. G. Variable susceptibility of mice to group B coxsackievirus infections. J Clin Microbiol. 1980 Jan;11(1):73–75. doi: 10.1128/jcm.11.1.73-75.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pelletier J., Kaplan G., Racaniello V. R., Sonenberg N. Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region. Mol Cell Biol. 1988 Mar;8(3):1103–1112. doi: 10.1128/mcb.8.3.1103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ray C. G., Minnich L. L., Johnson P. C. Selective polymyositis inducted by coxsackievirus B1 in mice. J Infect Dis. 1979 Aug;140(2):239–243. doi: 10.1093/infdis/140.2.239. [DOI] [PubMed] [Google Scholar]
  21. Roos R. P., Stein S., Ohara Y., Fu J. L., Semler B. L. Infectious cDNA clones of the DA strain of Theiler's murine encephalomyelitis virus. J Virol. 1989 Dec;63(12):5492–5496. doi: 10.1128/jvi.63.12.5492-5496.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Semler B. L., Johnson V. H., Tracy S. A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1777–1781. doi: 10.1073/pnas.83.6.1777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Strongwater S. L., Dorovini-Zis K., Ball R. D., Schnitzer T. J. A murine model of polymyositis induced by coxsackievirus B1 (Tucson strain). Arthritis Rheum. 1984 Apr;27(4):433–442. doi: 10.1002/art.1780270411. [DOI] [PubMed] [Google Scholar]
  24. Svitkin Y. V., Maslova S. V., Agol V. I. The genomes of attenuated and virulent poliovirus strains differ in their in vitro translation efficiencies. Virology. 1985 Dec;147(2):243–252. doi: 10.1016/0042-6822(85)90127-8. [DOI] [PubMed] [Google Scholar]
  25. Tolskaya E. A., Romanova L. I., Blinov V. M., Viktorova E. G., Sinyakov A. N., Kolesnikova M. S., Agol V. I. Studies on the recombination between RNA genomes of poliovirus: the primary structure and nonrandom distribution of crossover regions in the genomes of intertypic poliovirus recombinants. Virology. 1987 Nov;161(1):54–61. doi: 10.1016/0042-6822(87)90170-x. [DOI] [PubMed] [Google Scholar]
  26. Toniolo A., Onodera T., Jordan G., Yoon J. W., Notkins A. L. Virus-induced diabetes mellitus. Glucose abnormalities produced in mice by the six members of the Coxsackie B virus group. Diabetes. 1982 Jun;31(6 Pt 1):496–499. doi: 10.2337/diab.31.6.496. [DOI] [PubMed] [Google Scholar]
  27. Tu Z., Chapman N. M., Hufnagel G., Tracy S., Romero J. R., Barry W. H., Zhao L., Currey K., Shapiro B. The cardiovirulent phenotype of coxsackievirus B3 is determined at a single site in the genomic 5' nontranslated region. J Virol. 1995 Aug;69(8):4607–4618. doi: 10.1128/jvi.69.8.4607-4618.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Zhang G., Wilsden G., Knowles N. J., McCauley J. W. Complete nucleotide sequence of a coxsackie B5 virus and its relationship to swine vesicular disease virus. J Gen Virol. 1993 May;74(Pt 5):845–853. doi: 10.1099/0022-1317-74-5-845. [DOI] [PubMed] [Google Scholar]
  29. Zoll J., Galama J., Melchers W. Intratypic genome variability of the coxsackievirus B1 2A protease region. J Gen Virol. 1994 Mar;75(Pt 3):687–692. doi: 10.1099/0022-1317-75-3-687. [DOI] [PubMed] [Google Scholar]

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