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. 1983 Apr;111(1):27–34.

Differential expression of a lamininlike substance by high- and low-metastatic tumor cells.

J Varani, E J Lovett 3rd, J P McCoy Jr, S Shibata, D E Maddox, I J Goldstein, M Wicha
PMCID: PMC1916194  PMID: 6340517

Abstract

High-metastatic murine fibrosarcoma cells readily attached to Type IV (basement membrane) collagen, whereas low-metastatic cells isolated from the same tumor did not. The addition of laminin--a glycoprotein that facilitates the adherence of epithelial cells to their basement membranes--enhanced the attachment of the low-metastatic cells, but not the high-metastatic cells. Using anti-laminin antibodies and a laminin-binding lectin as probes, the authors were able to identify by immunofluorescence a moiety associated with the high-metastatic cells, but not the low-metastatic cells, which cross-reacted with murine laminin purified from the EHS sarcoma. When extracts from the high-metastatic cells were separated by affinity chromatography, with the laminin-binding lectin as the affinity substrate, a substance was isolated that had an apparent molecular weight of 56,000 daltons. The affinity-purified material reacted strongly with anti-laminin antibodies by enzyme-linked immunosorbent assay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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