Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1983 Aug;112(2):139–146.

Immunohistochemical localization of S-100 protein and peripheral nerve myelin proteins (P2 protein, P0 protein) in granular cell tumors.

M Mukai
PMCID: PMC1916267  PMID: 6192721

Abstract

The presence and distribution of nervous-system-specific protein (S-100 protein), peripheral nerve myelin proteins (P2 protein and P0 protein) that have not been given any attention in the field of tumor pathology, and striated muscle-related proteins (myoglobin and myosin) were studied in 18 cases of granular cell tumor by the peroxidase-antiperoxidase method. The granular cells of all cases were negatively stained with anti-striated-muscle-related protein antiserums. On the other hand, they were positively stained with anti-S-100 protein, P2 protein, and P0 protein antiserums. The distribution of P2 protein and P0 protein corresponded with that of characteristic PAS-positive granules on serial sections. Angular bodies in the interstitial cells were also positively stained with anti-P2 protein antiserum and anti-P0 protein antiserum. These results further support the hypothesis that the granular cell tumor is derived from Schwann cells and also suggest that myelin proteins are major core proteins of the characteristic granules and angular bodies of interstitial cells. The biologic significance of these results in relation to myelinogenesis is also discussed.

Full text

PDF
142

Images in this article

141Figure 1
on p.141
141Figure 2
on p.141
142Figure 3
on p.142
142Figure 4
on p.142
142Figure 5
on p.142
142Figure 6
on p.142
143Figure 7
on p.143
143Figure 8
on p.143
143Figure 9
on p.143
143Figure 10
on p.143
143Figure 11
on p.143
143Figure 12
on p.143
143Figure 13
on p.143
144Figure 14
on p.144

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alkek D. S., Johnson W. C., Graham J. H. Granular cell myoblastoma. A histological and enzymatic study. Arch Dermatol. 1968 Nov;98(5):543–547. [PubMed] [Google Scholar]
  2. Aparicio S. R., Lumsden C. E. Light- and electron-microscope studies on the granular cell myoblastoma of the tongue. J Pathol. 1969 Feb;97(2):339–355. doi: 10.1002/path.1710970221. [DOI] [PubMed] [Google Scholar]
  3. BANGLE R., Jr A morphological and histochemical study of the granular-cell myoblastoma. Cancer. 1952 Sep;5(5):950–965. doi: 10.1002/1097-0142(195209)5:5<950::aid-cncr2820050511>3.0.co;2-k. [DOI] [PubMed] [Google Scholar]
  4. Corson J. M., Pinkus G. S. Intracellular myoglobin--a specific marker for skeletal muscle differentiation in soft tissue sarcomas. An immunoperoxidase study. Am J Pathol. 1981 Jun;103(3):384–389. [PMC free article] [PubMed] [Google Scholar]
  5. Eylar E. H., Uyemura K., Brostoff S. W., Kitamura K., Ishaque A., Greenfield S. Proposed nomenclature for PNS myelin proteins. Neurochem Res. 1979 Apr;4(2):289–293. doi: 10.1007/BF00964152. [DOI] [PubMed] [Google Scholar]
  6. FISHER E. R., WECHSLER H. Granular cell myoblastoma--a misnomer. Electron microscopic and histochemical evidence concerning its Schwann cell derivation and nature (granular cell schwannoma). Cancer. 1962 Sep-Oct;15:936–954. doi: 10.1002/1097-0142(196209/10)15:5<936::aid-cncr2820150509>3.0.co;2-f. [DOI] [PubMed] [Google Scholar]
  7. Farris K. B., Faust B. F. Granular cell tumors of biliary ducts. Report of two cases and review of the literature. Arch Pathol Lab Med. 1979 Sep;103(10):510–512. [PubMed] [Google Scholar]
  8. Garancis J. C., Komorowski R. A., Kuzma J. F. Granular cell myoblastoma. Cancer. 1970 Mar;25(3):542–550. doi: 10.1002/1097-0142(197003)25:3<542::aid-cncr2820250308>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  9. HAISKEN W., LANGER E. [Submicroscopic structure of so-called myoblastic myoma (lipid fibroma, granular neuroma)]. Frankf Z Pathol. 1962;71:600–616. [PubMed] [Google Scholar]
  10. Johnson W., Jurand J., Hiramoto R. Immunohistologic studies of tumors containing myosin. Am J Pathol. 1965 Dec;47(6):1139–1155. [PMC free article] [PubMed] [Google Scholar]
  11. Kadlubowski M., Hughes R. A. Identification of the neuritogen for experimental allergic neuritis. Nature. 1979 Jan 11;277(5692):140–141. doi: 10.1038/277140a0. [DOI] [PubMed] [Google Scholar]
  12. Kitamura K., Suzuki A., Suzuki M., Uyemura K. Amino acid sequence of the glycopeptide derived from a major glycoprotein in bovine peripheral nerve myelin. FEBS Lett. 1979 Apr 1;100(1):67–70. doi: 10.1016/0014-5793(79)81132-1. [DOI] [PubMed] [Google Scholar]
  13. Kitamura K., Suzuki M., Suzuki A., Uyemura K. The complete amino acid sequence of the P2 protein in bovine peripheral nerve myelin. FEBS Lett. 1980 Jun 16;115(1):27–30. doi: 10.1016/0014-5793(80)80719-8. [DOI] [PubMed] [Google Scholar]
  14. Kitamura K., Suzuki M., Uyemura K. Purification and partial characterization of two glycoproteins in bovine peripheral nerve myelin membrane. Biochim Biophys Acta. 1976 Dec 14;455(3):806–816. doi: 10.1016/0005-2736(76)90050-x. [DOI] [PubMed] [Google Scholar]
  15. Mukai K., Rosai J., Hallaway B. E. Localization of myoglobin in normal and neoplastic human skeletal muscle cells using an immunoperoxidase method. Am J Surg Pathol. 1979 Aug;3(4):373–376. doi: 10.1097/00000478-197908000-00008. [DOI] [PubMed] [Google Scholar]
  16. Nakajima T., Watanabe S., Sato Y., Shimosato Y., Motoi M., Lennert K. S-100 protein in Langerhans cells, interdigitating reticulum cells and histiocytosis X cells. Gan. 1982 Jun;73(3):429–432. [PubMed] [Google Scholar]
  17. Nakazato Y., Ishizeki J., Takahashi K., Yamaguchi H. Immunohistochemical localization of S-100 protein in granular cell myoblastoma. Cancer. 1982 Apr 15;49(8):1624–1628. doi: 10.1002/1097-0142(19820415)49:8<1624::aid-cncr2820490816>3.0.co;2-h. [DOI] [PubMed] [Google Scholar]
  18. PEARSE A. G. E. The histogenesis of granular-cell myoblastoma (? granular-cell perineural fibroblastoma). J Pathol Bacteriol. 1950 Jul;62(3):351–362. doi: 10.1002/path.1700620306. [DOI] [PubMed] [Google Scholar]
  19. Shousha S., Lyssiotis T. Granular cell myoblastoma: positive staining for carcinoembryonic antigen. J Clin Pathol. 1979 Mar;32(3):219–224. doi: 10.1136/jcp.32.3.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sobel H. J., Marquet E., Avrin E., Schwarz R. Granular cell myoblastoma. An electron microscopic and cytochemical study illustrating the genesis of granules and aging of myoblastoma cells. Am J Pathol. 1971 Oct;65(1):59–78. [PMC free article] [PubMed] [Google Scholar]
  21. Sobel H. J., Schwarz R., Marquet E. Light- and electron-microscope study of the origin of granular-cell myoblastoma. J Pathol. 1973 Feb;109(2):101–111. doi: 10.1002/path.1711090204. [DOI] [PubMed] [Google Scholar]
  22. Stefansson K., Wollmann R. L. S-100 protein in granular cell tumors (granular cell myoblastomas). Cancer. 1982 May 1;49(9):1834–1838. doi: 10.1002/1097-0142(19820501)49:9<1834::aid-cncr2820490916>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  23. Stefansson K., Wollmann R., Jerkovic M. S-100 protein in soft-tissue tumors derived from Schwann cells and melanocytes. Am J Pathol. 1982 Feb;106(2):261–268. [PMC free article] [PubMed] [Google Scholar]
  24. Sternberger L. A., Hardy P. H., Jr, Cuculis J. J., Meyer H. G. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem. 1970 May;18(5):315–333. doi: 10.1177/18.5.315. [DOI] [PubMed] [Google Scholar]
  25. Suzuki M., Kitamura K., Uyemura K., Ogawa Y., Ishihara Y., Matsuyama H. Neuritogenic activity of peripheral nerve myelin proteins in Lewis rats. Neurosci Lett. 1980 Oct 2;19(3):353–358. doi: 10.1016/0304-3940(80)90287-6. [DOI] [PubMed] [Google Scholar]
  26. Usui M., Ishii S., Yamawaki S., Sasaki T., Minami A. Malignant granular cell tumor of the radial nerve: an autopsy observation with electron microscopic and tissue culture studies. Cancer. 1977 Apr;39(4):1547–1555. doi: 10.1002/1097-0142(197704)39:4<1547::aid-cncr2820390428>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
  27. Uyemura K., Kato-Yamanaka T., Kitamura K. Distribution and optical activity of the basic protein in bovine peripheral nerve myelin. J Neurochem. 1977 Jul;29(1):61–68. doi: 10.1111/j.1471-4159.1977.tb03924.x. [DOI] [PubMed] [Google Scholar]
  28. Uyemura K., Suzuki M., Kitamura K., Horie K., Ogawa Y., Matsuyama H., Nozaki S., Muramatsu I. Neuritogenic determinant of bovine P2 protein in peripheral nerve myelin. J Neurochem. 1982 Sep;39(3):895–898. doi: 10.1111/j.1471-4159.1982.tb07979.x. [DOI] [PubMed] [Google Scholar]
  29. Uyemura K., Vincendon G., Gombos G., Mandel P. Purification and some properties of S-100 protein fractions from sheep and pig brains. J Neurochem. 1971 Mar;18(3):429–438. doi: 10.1111/j.1471-4159.1971.tb11970.x. [DOI] [PubMed] [Google Scholar]
  30. WAKSMAN B. H., ADAMS R. D. Allergic neuritis: an experimental disease of rabbits induced by the injection of peripheral nervous tissue and adjuvants. J Exp Med. 1955 Aug 1;102(2):213–236. doi: 10.1084/jem.102.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. al-Sarraf M., Loud A. V., Vaitkevicius V. K. Malignant granular cell tumor. Histochemical and electron microscopic study. Arch Pathol. 1971 Jun;91(6):550–558. [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES