Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1985 Aug;85(4):933–941. doi: 10.1111/j.1476-5381.1985.tb11094.x

Identification of presynaptic beta 2-adrenoceptors on the sympathetic nerve fibres of the human pulmonary artery.

M Göthert, F Hentrich
PMCID: PMC1916672  PMID: 2994790

Abstract

Strips of human pulmonary arteries from patients undergoing surgery for lung tumour were incubated with [3H]-noradrenaline. Subsequently, they were superfused with physiological salt solution containing cocaine and corticosterone. Tritium overflow from the strips was stimulated by transmural electrical impulses (2 Hz). The electrically evoked overflow of tritium consisted of 91% unmetabolized [3H]-noradrenaline, and this percentage was not altered by isoprenaline. Adrenaline (in the presence of rauwolscine), isoprenaline and the preferential beta 2-adrenoceptor agonist, procaterol, concentration-dependently increased the electrically evoked tritium overflow. Prenalterol, a beta-adrenoceptor agonist with moderate preference for beta 1-adrenoceptors, was considerably less active than the previously mentioned agonists; noradrenaline (in the presence of rauwolscine) was ineffective. The concentration-response curve of procaterol was shifted to the right by the preferential beta 2-adrenoceptor antagonist ICI 118-551 but was not affected by the beta 1-selective antagonist, atenolol. Propranolol, but not atenolol, produced a shift to the right of the concentration-response curve of isoprenaline. It is concluded that the sympathetic nerve fibres of the human pulmonary artery are endowed with facilitatory presynaptic beta 2-adrenoceptors.

Full text

PDF
939

Images in this article

938Figure 6
on p.938

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler-Graschinsky E., Langer S. Z. Possible role of a beta-adrenoceptor in the regulation of noradrenaline release by nerve stimulation through a positive feed-back mechanism. Br J Pharmacol. 1975 Jan;53(1):43–50. doi: 10.1111/j.1476-5381.1975.tb07328.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barrett A. M., Carter J., Fitzgerald J. D., Hull R., Le Count D. A new type of cardioselective adrenoceptive blocking drug. Br J Pharmacol. 1973 Jun;48(2):340P–340P. [PMC free article] [PubMed] [Google Scholar]
  3. Brodde O. E., Kuhlhoff F., Arroyo J., Prywarra A. No evidence for temperature-dependent changes in the pharmacological specificity of beta 1- and beta 2-adrenoceptors in rabbit lung membranes. Naunyn Schmiedebergs Arch Pharmacol. 1983 Feb;322(1):20–28. doi: 10.1007/BF00649347. [DOI] [PubMed] [Google Scholar]
  4. Carlsson E., Dahlöf C. G., Hedberg A., Persson H., Tångstrand B. Differentiation of cardiac chronotropic and inotropic effects of beta-adrenoceptor agonists. Naunyn Schmiedebergs Arch Pharmacol. 1977 Nov;300(2):101–105. doi: 10.1007/BF00505039. [DOI] [PubMed] [Google Scholar]
  5. Cook N., Richardson A., Barnett D. B. Comparison of the beta 1 selective affinity of prenalterol and corwin demonstrated by radioligand binding. Eur J Pharmacol. 1984 Mar 2;98(3-4):407–412. doi: 10.1016/0014-2999(84)90289-9. [DOI] [PubMed] [Google Scholar]
  6. Dahlöf C., Ljung B., Ablad B. Pre- and postjunctional beta-adrenoceptor mediated effects on transmitter release and effector response in the isolated rat portal vein. Acta Physiol Scand. 1980 Jan;108(1):39–47. doi: 10.1111/j.1748-1716.1980.tb06498.x. [DOI] [PubMed] [Google Scholar]
  7. Endo T., Starke K., Bangerter A., Taube H. D. Presynaptic receptor systems on the noradrenergic neurones of the rabbit pulmonary artery. Naunyn Schmiedebergs Arch Pharmacol. 1977 Feb;296(3):229–247. doi: 10.1007/BF00498689. [DOI] [PubMed] [Google Scholar]
  8. Freeman W. K., Rorie D. K., Tyce G. M. Effects of 5-hydroxytryptamine on neuroeffector junction in human pulmonary artery. J Appl Physiol Respir Environ Exerc Physiol. 1981 Sep;51(3):693–698. doi: 10.1152/jappl.1981.51.3.693. [DOI] [PubMed] [Google Scholar]
  9. Furchgott R. F. The pharmacological differentiation of adrenergic receptors. Ann N Y Acad Sci. 1967 Feb 10;139(3):553–570. doi: 10.1111/j.1749-6632.1967.tb41229.x. [DOI] [PubMed] [Google Scholar]
  10. Graffe K. H., Stefano F. J., Langer S. Z. Preferential metabolism of (-) 3 H-norepinephrine through the deaminated glycol in the rat vas deferens. Biochem Pharmacol. 1973 May 15;22(10):1147–1160. doi: 10.1016/0006-2952(73)90231-1. [DOI] [PubMed] [Google Scholar]
  11. Guimarães S., Brandão F., Paiva M. Q. A study of the adrenoceptor-mediated feedback mechanism by using adrenaline as a false transmitter. Naunyn Schmiedebergs Arch Pharmacol. 1978 Nov;305(2):185–188. doi: 10.1007/BF00508291. [DOI] [PubMed] [Google Scholar]
  12. Göthert M. Facilitatory effect of adrenocorticotropic hormone and related peptides on Ca2+-dependent noradrenaline release from sympathetic nerves. Neuroscience. 1984 Apr;11(4):1001–1009. doi: 10.1016/0306-4522(84)90210-0. [DOI] [PubMed] [Google Scholar]
  13. Göthert M., Schlicker E., Hentrich F., Rohm N., Zerkowski H. R. Modulation of noradrenaline release in human saphenous vein via presynaptic alpha 2-adrenoceptors. Eur J Pharmacol. 1984 Jul 13;102(2):261–267. doi: 10.1016/0014-2999(84)90257-7. [DOI] [PubMed] [Google Scholar]
  14. Hedqvist P., Moawad A. Presynaptic alpha- and beta-adrenoceptor medicated control of noradrenaline release in human oviduct. Acta Physiol Scand. 1975 Dec;95(4):494–496. doi: 10.1111/j.1748-1716.1975.tb10079.x. [DOI] [PubMed] [Google Scholar]
  15. Johansson U., Waldeck B. On the stereospecificity of the beta2-adrenoceptor blocking properties of prenalterol. J Pharm Pharmacol. 1980 Sep;32(9):659–660. doi: 10.1111/j.2042-7158.1980.tb13029.x. [DOI] [PubMed] [Google Scholar]
  16. Lands A. M., Arnold A., McAuliff J. P., Luduena F. P., Brown T. G., Jr Differentiation of receptor systems activated by sympathomimetic amines. Nature. 1967 May 6;214(5088):597–598. doi: 10.1038/214597a0. [DOI] [PubMed] [Google Scholar]
  17. Langer S. Z. Presynaptic regulation of the release of catecholamines. Pharmacol Rev. 1980 Dec;32(4):337–362. [PubMed] [Google Scholar]
  18. Langer S. Z. Sixth gaddum memorial lecture, National Institute for Medical Research, Mill Hill, January 1977. Presynaptic receptors and their role in the regulation of transmitter release. Br J Pharmacol. 1977 Aug;60(4):481–497. doi: 10.1111/j.1476-5381.1977.tb07526.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Majewski H., McCulloch M. W., Rand M. J., Story D. F. Adrenaline activation of prejunctional beta-adrenoceptors in guinea-pig atria. Br J Pharmacol. 1980;71(2):435–444. doi: 10.1111/j.1476-5381.1980.tb10956.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Majewski H. Modulation of noradrenaline release through activation of presynaptic beta-adrenoreceptors. J Auton Pharmacol. 1983 Mar;3(1):47–60. doi: 10.1111/j.1474-8673.1983.tb00496.x. [DOI] [PubMed] [Google Scholar]
  21. Misu Y., Kaiho M., Ogawa K., Kubo T. Adrenergic transmission failure via the blockade of presynaptic beta receptors in guinea-pig pulmonary arteries. J Pharmacol Exp Ther. 1981 Jul;218(1):242–247. [PubMed] [Google Scholar]
  22. Misu Y., Kuwahara M., Kaiho M., Kubo T. Further characterization of presynaptic beta-adrenoceptors in guinea-pig pulmonary arteries. Eur J Pharmacol. 1983 Jul 22;91(2-3):287–290. doi: 10.1016/0014-2999(83)90480-6. [DOI] [PubMed] [Google Scholar]
  23. Moulds R. F., Stevens M. J. Facilitatory prejunctional beta-adrenoceptors in human arteries and veins. Gen Pharmacol. 1983;14(1):81–83. doi: 10.1016/0306-3623(83)90069-1. [DOI] [PubMed] [Google Scholar]
  24. Nahorski S. R., Howlett D. R., Redgrave P. Loss of beta-adrenoceptor binding sites in rat striatum following kainic acid lesions. Eur J Pharmacol. 1979 Dec 7;60(2-3):249–252. doi: 10.1016/0014-2999(79)90226-7. [DOI] [PubMed] [Google Scholar]
  25. Rand M., Law M., Story D. F., McCulloch M. W. Effects of beta-adrenoreceptor blocking drugs on adrenergic transmission. Drugs. 1976;11(Suppl 1):134–143. doi: 10.2165/00003495-197600111-00029. [DOI] [PubMed] [Google Scholar]
  26. Starke K. Presynaptic receptors. Annu Rev Pharmacol Toxicol. 1981;21:7–30. doi: 10.1146/annurev.pa.21.040181.000255. [DOI] [PubMed] [Google Scholar]
  27. Starke K. Regulation of noradrenaline release by presynaptic receptor systems. Rev Physiol Biochem Pharmacol. 1977;77:1–124. doi: 10.1007/BFb0050157. [DOI] [PubMed] [Google Scholar]
  28. Stevens M. J., Rittinghausen R. E., Medcalf R. L., Moulds R. F. Prejunctional beta-adrenoceptors in human digital arteries. Eur J Pharmacol. 1982 Sep 24;83(3-4):263–270. doi: 10.1016/0014-2999(82)90259-x. [DOI] [PubMed] [Google Scholar]
  29. Stj-5aarne L., Brundin J. Dual adreoceptor-mediated control of noradrenaline secretion from human vasoconstrictor nerves: facilitation by BETA-receptors and inhibitor by alpha-receptors. Acta Physiol Scand. 1975 May;94(1):139–141. doi: 10.1111/j.1748-1716.1975.tb05872.x. [DOI] [PubMed] [Google Scholar]
  30. Stjärne L., Brundin J. Beta2-adrenoceptors facilitating noradrenaline secretion from human vasoconstrictor nerves. Acta Physiol Scand. 1976 Mar;97(1):88–93. doi: 10.1111/j.1748-1716.1976.tb10238.x. [DOI] [PubMed] [Google Scholar]
  31. Vincent H. H., Man In't Veld A. J., Boomsma F., Wenting G. J., Schalekamp M. A. Elevated plasma noradrenaline in response to beta-adrenoceptor stimulation in man. Br J Clin Pharmacol. 1982 May;13(5):717–721. doi: 10.1111/j.1365-2125.1982.tb01442.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Westfall T. C., Peach M. J., Tittermary V. Enhancement of the electrically induced release of norepinephrine from the rat portal vein: mediation by beta 2-adrenoceptors. Eur J Pharmacol. 1979 Sep 1;58(1):67–74. doi: 10.1016/0014-2999(79)90341-8. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES