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. 1985 Sep;86(1):117–124. doi: 10.1111/j.1476-5381.1985.tb09441.x

The effects of sodium nitroprusside and 8-bromo-cyclic GMP on electrical and mechanical activities of the rat tail artery.

D W Cheung, M J MacKay
PMCID: PMC1916861  PMID: 2996672

Abstract

The effects of sodium nitroprusside and 8-bromo-guanosine 3':5'-cyclic monophosphate (8-bromocyclic GMP) on the electrical and mechanical activities of the rat tail artery were compared. The inhibitory effects of sodium nitroprusside on the contractions induced by noradrenaline, phenylephrine, KC1 and clonidine were mimicked by 8-bromo-cyclic GMP. Sodium nitroprusside and 8-bromo-cyclic GMP increased the resting membrane potential only in preparations with low initial resting membrane potentials. In tissues previously contracted and depolarized with noradrenaline, KC1 and clonidine, both sodium nitroprusside and 8-bromo-cyclic GMP caused relaxation without significantly affecting the membrane potential. Both sodium nitroprusside and 8-bromo-cyclic GMP abolished neurally-mediated contractions without any significant effect on the electrical responses. These results suggest that the actions of sodium nitroprusside and 8-bromo-cyclic GMP are not related to membrane hyperpolarization or inhibition of membrane excitability.

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Selected References

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  1. Bond M., Kitazawa T., Somlyo A. P., Somlyo A. V. Release and recycling of calcium by the sarcoplasmic reticulum in guinea-pig portal vein smooth muscle. J Physiol. 1984 Oct;355:677–695. doi: 10.1113/jphysiol.1984.sp015445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cheung D. W. Neural regulation of electrical and mechanical activities in the rat tail artery. Pflugers Arch. 1984 Mar;400(3):335–337. doi: 10.1007/BF00581570. [DOI] [PubMed] [Google Scholar]
  3. Edwards J. C., Ignarro L. J., Hyman A. L., Kadowitz P. J. Relaxation of intrapulmonary artery and vein by nitrogen oxide-containing vasodilators and cyclic GMP. J Pharmacol Exp Ther. 1984 Jan;228(1):33–42. [PubMed] [Google Scholar]
  4. Heaslip R. J., Rahwan R. G. Norepinephrine-induced contractions of the rat aorta in the absence of extracellular calcium--III. Effects of cyclic nucleotides. Gen Pharmacol. 1983;14(5):513–518. doi: 10.1016/0306-3623(83)90111-8. [DOI] [PubMed] [Google Scholar]
  5. Hester R. K., Weiss G. B. Effects of nitroprusside and D 600 on norepinephrine- and KCl-stimulated Ca++ activation and contraction systems in canine renal vein as compared to canine renal artery. J Cardiovasc Pharmacol. 1984 Sep-Oct;6(5):762–771. doi: 10.1097/00005344-198409000-00005. [DOI] [PubMed] [Google Scholar]
  6. Hirst G. D., van Helden D. F. Ionic basis of the resting potential of submucosal arterioles in the ileum of the guinea-pig. J Physiol. 1982 Dec;333:53–67. doi: 10.1113/jphysiol.1982.sp014438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Imai S., Nakazawa M., Imai H., Nabata H. Effects of procaine on the isolated dog coronary artery. Arch Int Pharmacodyn Ther. 1984 Sep;271(1):98–105. [PubMed] [Google Scholar]
  8. Ito Y., Suzuki H., Kuriyama H. Effects of sodium nitroprusside on smooth muscle cells of rabbit pulmonary artery and portal vein. J Pharmacol Exp Ther. 1978 Dec;207(3):1022–1031. [PubMed] [Google Scholar]
  9. Itoh T., Kajiwara M., Kitamura K., Kuriyama H. Effects of vasodilator agents on smooth muscle cells of the coronary artery of the pig. Br J Pharmacol. 1981 Oct;74(2):455–468. doi: 10.1111/j.1476-5381.1981.tb09991.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Itoh T., Kajiwara M., Kitamura K., Kuriyama H. Roles of stored calcium on the mechanical response evoked in smooth muscle cells of the porcine coronary artery. J Physiol. 1982 Jan;322:107–125. doi: 10.1113/jphysiol.1982.sp014026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Karaki H., Nakagawa H., Urakawa N. Comparative effects of verapamil and sodium nitroprusside on contraction and 45Ca uptake in the smooth muscle of rabbit aorta, rat aorta and guinea-pig taenia coli. Br J Pharmacol. 1984 Feb;81(2):393–400. doi: 10.1111/j.1476-5381.1984.tb10091.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Katsuki S., Arnold W. P., Murad F. Effects of sodium nitroprusside, nitroglycerin, and sodium azide on levels of cyclic nucleotides and mechanical activity of various tissues. J Cyclic Nucleotide Res. 1977 Aug;3(4):239–247. [PubMed] [Google Scholar]
  13. Keith R. A., Burkman A. M., Sokoloski T. D., Fertel R. H. Vascular tolerance to nitroglycerin and cyclic GMP generation in rat aortic smooth muscle. J Pharmacol Exp Ther. 1982 Jun;221(3):525–531. [PubMed] [Google Scholar]
  14. Kreye V. A., Baron G. D., Lüth J. B., Schmidt-Gayk H. Mode of action of sodium nitroprusside on vascular smooth muscle. Naunyn Schmiedebergs Arch Pharmacol. 1975;288(4):381–402. doi: 10.1007/BF00501284. [DOI] [PubMed] [Google Scholar]
  15. PAGE I. H., CORCORAN A. C., DUSTAN H. P., KOPPANYI T. Cardiovascular actions of sodium nitroprusside in animals and hypertensive patients. Circulation. 1955 Feb;11(2):188–198. doi: 10.1161/01.cir.11.2.188. [DOI] [PubMed] [Google Scholar]
  16. Schultz K., Schultz K., Schultz G. Sodium nitroprusside and other smooth muscle-relaxants increase cyclic GMP levels in rat ductus deferens. Nature. 1977 Feb 24;265(5596):750–751. doi: 10.1038/265750a0. [DOI] [PubMed] [Google Scholar]
  17. Verhaeghe R. H., Shepherd J. T. Effect of nitroprusside on smooth muscle and adrenergic nerve terminals in isolated blood vessels. J Pharmacol Exp Ther. 1976 Oct;199(1):269–277. [PubMed] [Google Scholar]

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