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. 1987 Feb;90(2):413–419. doi: 10.1111/j.1476-5381.1987.tb08971.x

Crystal-induced inflammation in the rat subcutaneous air-pouch.

P M Brooks, D Burton, M J Forrest
PMCID: PMC1916951  PMID: 3030485

Abstract

Monosodium urate (MSU) and calcium pyrophosphate dihydrate (CPPD) crystals initiated acute inflammatory reactions characterized by increased plasma extravasation and polymorphonuclear leukocyte (PMNL) accumulation in the rat subcutaneous air-pouch. Pretreatment of rats with colchicine (1 mg kg-1, s.c.) inhibited PMNL accumulation induced by either crystal type but had a greater inhibitory effect on MSU-induced plasma extravasation compared with that induced by CPPD crystals. Colchicine (1 mg kg-1, s.c.) did not reduce histamine-induced plasma extravasation in the air-pouch. The lipoxygenase product of arachidonic acid metabolism, leukotriene B4 (LTB4), was detected in MSU-induced exudates but not in CPPD-induced exudates. Pretreatment of rats with colchicine (1 mg kg-1, s.c.) inhibited LTB4 production in MSU-induced exudates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhattacherjee P., Hammond B., Salmon J. A., Stepney R., Eakins K. E. Chemotactic response to some arachidonic acid lipoxygenase products in the rabbit eye. Eur J Pharmacol. 1981 Jul 17;73(1):21–28. doi: 10.1016/0014-2999(81)90141-2. [DOI] [PubMed] [Google Scholar]
  2. Björk J., Hedqvist P., Arfors K. E. Increase in vascular permeability induced by leukotriene B4 and the role of polymorphonuclear leukocytes. Inflammation. 1982 Jun;6(2):189–200. doi: 10.1007/BF00916243. [DOI] [PubMed] [Google Scholar]
  3. Borgeat P., Hamberg M., Samuelsson B. Transformation of arachidonic acid and homo-gamma-linolenic acid by rabbit polymorphonuclear leukocytes. Monohydroxy acids from novel lipoxygenases. J Biol Chem. 1976 Dec 25;251(24):7816–7820. [PubMed] [Google Scholar]
  4. Bray M. A., Cunningham F. M., Ford-Hutchinson A. W., Smith M. J. Leukotriene B4: a mediator of vascular permeability. Br J Pharmacol. 1981 Mar;72(3):483–486. doi: 10.1111/j.1476-5381.1981.tb11000.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bray M. A., Ford-Hutchinson A. W., Smith M. J. Leukotriene B4: an inflammatory mediator in vivo. Prostaglandins. 1981 Aug;22(2):213–222. doi: 10.1016/0090-6980(81)90036-8. [DOI] [PubMed] [Google Scholar]
  6. Denko C. W., Whitehouse M. W. Experimental inflammation induced by naturally occurring microcrystalline calcium salts. J Rheumatol. 1976 Mar;3(1):54–62. [PubMed] [Google Scholar]
  7. Edwards J. C., Sedgwick A. D., Willoughby D. A. The formation of a structure with the features of synovial lining by subcutaneous injection of air: an in vivo tissue culture system. J Pathol. 1981 Jun;134(2):147–156. doi: 10.1002/path.1711340205. [DOI] [PubMed] [Google Scholar]
  8. Glatt M., Dieppe P., Willoughby D. Crystal-induced inflammation, enzyme release and the effects of drugs in the rat pleural space. J Rheumatol. 1979 May-Jun;6(3):251–258. [PubMed] [Google Scholar]
  9. Goetzl E. J., Pickett W. C. The human PMN leukocyte chemotactic activity of complex hydroxy-eicosatetraenoic acids (HETEs). J Immunol. 1980 Oct;125(4):1789–1791. [PubMed] [Google Scholar]
  10. KOHN N. N., HUGHES R. E., McCARTY D. J., Jr, FAIRES J. S. The significance of calcium phosphate crystals in the synovial fluid of arthritic patients: the "pseudogout syndrome". II. Identification of crystals. Ann Intern Med. 1962 May;56:738–745. doi: 10.7326/0003-4819-56-5-738. [DOI] [PubMed] [Google Scholar]
  11. MCCARTY D. J., HOLLANDER J. L. Identification of urate crystals in gouty synovial fluid. Ann Intern Med. 1961 Mar;54:452–460. doi: 10.7326/0003-4819-54-3-452. [DOI] [PubMed] [Google Scholar]
  12. McCarty D. J., Jr, Phelps P., Pyenson J. Crystal-induced inflammation in canine joints. I. An experimental model with quantification of the host response. J Exp Med. 1966 Jul 1;124(1):99–114. doi: 10.1084/jem.124.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Palmer R. M., Stepney R. J., Higgs G. A., Eakins K. E. Chemokinetic activity of arachidonic and lipoxygenase products on leuocyctes of different species. Prostaglandins. 1980 Aug;20(2):411–418. doi: 10.1016/s0090-6980(80)80058-x. [DOI] [PubMed] [Google Scholar]
  14. Rae S. A., Davidson E. M., Smith M. J. Leukotriene B4, an inflammatory mediator in gout. Lancet. 1982 Nov 20;2(8308):1122–1124. doi: 10.1016/s0140-6736(82)92785-4. [DOI] [PubMed] [Google Scholar]
  15. Reibman J., Haines K. A., Rich A. M., Cristello P., Giedd K. N., Weissmann G. Colchicine inhibits ionophore-induced formation of leukotriene B4 by human neutrophils: the role of microtubules. J Immunol. 1986 Feb 1;136(3):1027–1032. [PubMed] [Google Scholar]
  16. Serhan C. N., Lundberg U., Weissmann G., Samuelsson B. Formation of leukotrienes and hydroxy acids by human neutrophils and platelets exposed to monosodium urate. Prostaglandins. 1984 Apr;27(4):563–581. doi: 10.1016/0090-6980(84)90092-3. [DOI] [PubMed] [Google Scholar]
  17. Simmons P. M., Salmon J. A., Moncada S. The release of leukotriene B4 during experimental inflammation. Biochem Pharmacol. 1983 Apr 15;32(8):1353–1359. doi: 10.1016/0006-2952(83)90446-x. [DOI] [PubMed] [Google Scholar]
  18. Smith M. J., Ford-Hutchinson A. W., Bray M. A. Leukotriene B: a potential mediator of inflammation. J Pharm Pharmacol. 1980 Jul;32(7):517–518. doi: 10.1111/j.2042-7158.1980.tb12985.x. [DOI] [PubMed] [Google Scholar]
  19. Vinegar R., Truax J. F., Selph J. L., Johnston P. R., Venable A. L., Voelker F. A. Development of carrageenan pleurisy in the rat: effects of colchicine on inhibition of cell mobilization. Proc Soc Exp Biol Med. 1981 Oct;168(1):24–32. doi: 10.3181/00379727-168-41229. [DOI] [PubMed] [Google Scholar]
  20. Wallace S. I., Bernstein D., Diamond H. Diagnostic value of the colchicine therapeutic trial. JAMA. 1967 Feb 20;199(8):525–528. [PubMed] [Google Scholar]
  21. Wedmore C. V., Williams T. J. Control of vascular permeability by polymorphonuclear leukocytes in inflammation. Nature. 1981 Feb 19;289(5799):646–650. doi: 10.1038/289646a0. [DOI] [PubMed] [Google Scholar]

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