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. 1987 Jan;90(1):111–120. doi: 10.1111/j.1476-5381.1987.tb16830.x

A pharmacological study of the rabbit saphenous artery in vitro: a vessel with a large purinergic contractile response to sympathetic nerve stimulation.

G Burnstock, J J Warland
PMCID: PMC1917280  PMID: 3814914

Abstract

Mechanical responses to transmural electrical stimulation were recorded in isolated transverse ring preparations of rabbit saphenous artery. Electrical stimulation for a period of 1 s produced a rapid monophasic contraction and, for a period of 1 min, a biphasic contraction consisting of a rapid constriction followed by a slower sustained constriction. All contractions were abolished in the presence of tetrodotoxin (1 microgram ml-1) or guanethidine (4 microM). After desensitization of the P2-purinoceptor with alpha,beta-methylene ATP, contractions to electrical stimulation for 1 s were reduced significantly at all frequencies tested: responses evoked by stimulation at 4 Hz were usually almost completely inhibited, whereas those evoked by stimulation at 64 Hz were only partially inhibited. On the other hand, in the presence of the alpha-adrenoceptor antagonist, prazosin, neurogenic contractions were only partially reduced: at 4 Hz there was no significant reduction in the neurogenic contractions while at 32 and 64 Hz, contractions were reduced by an average of 20 and 28% respectively. Usually all contractions were abolished by a combination of the two drugs. Prazosin antagonized contractions of the vessel to exogenously applied noradrenaline but not to ATP, whereas desensitization of the P2-purinoceptor with alpha,beta-methylene ATP blocked responses to ATP but not those to noradrenaline. The concentration response curve to histamine was not affected by treatment of the vessel with prazosin, or after desensitization of the P2-purinoceptor with alpha,beta-methylene ATP. These results suggest that noradrenaline and ATP are co-released from sympathetic nerves supplying the rabbit saphenous artery, both substances being involved in the mechanical contractions of this tissue. Further, the ratio of ATP to noradrenaline involved in these mechanical contractions is dependent upon the frequency of stimulation, but at all frequencies tested the purinergic component is greater than the adrenergic component.

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Selected References

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  1. BULBRING E. Measurements of oxygen consumption in smooth muscle. J Physiol. 1953 Oct;122(1):111–134. doi: 10.1113/jphysiol.1953.sp004983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bevan J. A., Osher J. V. A direct method for recording tension changes in the wall of small blood vessels in vitro. Agents Actions. 1972;2(5):257–260. doi: 10.1007/BF02087051. [DOI] [PubMed] [Google Scholar]
  3. Burnstock G. Do some nerve cells release more than one transmitter? Neuroscience. 1976 Aug;1(4):239–248. doi: 10.1016/0306-4522(76)90054-3. [DOI] [PubMed] [Google Scholar]
  4. Burnstock G. Evolution of the autonomic innervation of visceral and cardiovascular systems in vertebrates. Pharmacol Rev. 1969 Dec;21(4):247–324. [PubMed] [Google Scholar]
  5. Burnstock G., Kennedy C. A dual function for adenosine 5'-triphosphate in the regulation of vascular tone. Excitatory cotransmitter with noradrenaline from perivascular nerves and locally released inhibitory intravascular agent. Circ Res. 1986 Mar;58(3):319–330. doi: 10.1161/01.res.58.3.319. [DOI] [PubMed] [Google Scholar]
  6. Burnstock G., Kennedy C. Is there a basis for distinguishing two types of P2-purinoceptor? Gen Pharmacol. 1985;16(5):433–440. doi: 10.1016/0306-3623(85)90001-1. [DOI] [PubMed] [Google Scholar]
  7. Burnstock G. Purinergic nerves. Pharmacol Rev. 1972 Sep;24(3):509–581. [PubMed] [Google Scholar]
  8. Cavero I., Roach A. G. The pharmacology of prazosin, a novel antihypertensive agent. Life Sci. 1980 Oct 27;27(17):1525–1540. doi: 10.1016/0024-3205(80)90561-5. [DOI] [PubMed] [Google Scholar]
  9. Duval N., Hicks P. E., Langer S. Z. Inhibitory effects of alpha, beta-methylene ATP on nerve-mediated contractions of the nictitating membrane in reserpinised cats. Eur J Pharmacol. 1985 Apr 16;110(3):373–377. doi: 10.1016/0014-2999(85)90567-9. [DOI] [PubMed] [Google Scholar]
  10. Fedan J. S., Hogaboom G. K., O'Donnell J. P., Colby J., Westfall D. P. Contribution by purines to the neurogenic response of the vas deferens of the guinea pig. Eur J Pharmacol. 1981 Jan 5;69(1):41–53. doi: 10.1016/0014-2999(81)90600-2. [DOI] [PubMed] [Google Scholar]
  11. Gillespie J. S., Rae R. M. Constrictor and compliance responses of some arteries to nerve or drug stimulation. J Physiol. 1972 May;223(1):109–130. doi: 10.1113/jphysiol.1972.sp009837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Head R. J., Stitzel R. E., de la Lande I. S., Johnson S. M. Effect of chronic denervation on the activities of monoamine oxidase and catechol-O-methyl transferase and on the contents of noradrenaline and adenosine triphosphate in the rabbit ear artery. Blood Vessels. 1977;14(4):229–239. doi: 10.1159/000158131. [DOI] [PubMed] [Google Scholar]
  13. Holman M. E., Surprenant A. An electrophysiological analysis of the effects of noradrenaline and alpha-receptor antagonists on neuromuscular transmission in mammalian muscular arteries. Br J Pharmacol. 1980;71(2):651–661. doi: 10.1111/j.1476-5381.1980.tb10986.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ishikawa S. Actions of ATP and alpha, beta-methylene ATP on neuromuscular transmission and smooth muscle membrane of the rabbit and guinea-pig mesenteric arteries. Br J Pharmacol. 1985 Dec;86(4):777–787. doi: 10.1111/j.1476-5381.1985.tb11099.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Katsuragi T., Su C. Facilitation by clonidine of purine release induced by high KCl from the rabbit pulmonary artery. Br J Pharmacol. 1981 Nov;74(3):709–713. doi: 10.1111/j.1476-5381.1981.tb10482.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kennedy C., Saville V. L., Burnstock G. The contributions of noradrenaline and ATP to the responses of the rabbit central ear artery to sympathetic nerve stimulation depend on the parameters of stimulation. Eur J Pharmacol. 1986 Apr 2;122(3):291–300. doi: 10.1016/0014-2999(86)90409-7. [DOI] [PubMed] [Google Scholar]
  17. Meldrum L. A., Burnstock G. Evidence that ATP acts as a co-transmitter with noradrenaline in sympathetic nerves supplying the guinea-pig vas deferens. Eur J Pharmacol. 1983 Aug 19;92(1-2):161–163. doi: 10.1016/0014-2999(83)90126-7. [DOI] [PubMed] [Google Scholar]
  18. Moss H. E., Burnstock G. A comparative study of electrical field stimulation of the guinea-pig, ferret and marmoset urinary bladder. Eur J Pharmacol. 1985 Aug 27;114(3):311–316. doi: 10.1016/0014-2999(85)90375-9. [DOI] [PubMed] [Google Scholar]
  19. Muramatsu I. Evidence for sympathetic, purinergic transmission in the mesenteric artery of the dog. Br J Pharmacol. 1986 Mar;87(3):478–480. doi: 10.1111/j.1476-5381.1986.tb10187.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Muramatsu I., Fujiwara M., Miura A., Sakakibara Y. Possible involvement of adenine nucleotides in sympathetic neuroeffector mechanisms of dog basilar artery. J Pharmacol Exp Ther. 1981 Feb;216(2):401–409. [PubMed] [Google Scholar]
  21. Sneddon P., Burnstock G. ATP as a co-transmitter in rat tail artery. Eur J Pharmacol. 1984 Oct 30;106(1):149–152. doi: 10.1016/0014-2999(84)90688-5. [DOI] [PubMed] [Google Scholar]
  22. Sneddon P., Burnstock G. Inhibition of excitatory junction potentials in guinea-pig vas deferens by alpha, beta-methylene-ATP: further evidence for ATP and noradrenaline as cotransmitters. Eur J Pharmacol. 1984 Apr 13;100(1):85–90. doi: 10.1016/0014-2999(84)90318-2. [DOI] [PubMed] [Google Scholar]
  23. Sneddon P., Westfall D. P., Fedan J. S. Cotransmitters in the motor nerves of the guinea pig vas deferens: electrophysiological evidence. Science. 1982 Nov 12;218(4573):693–695. doi: 10.1126/science.6291151. [DOI] [PubMed] [Google Scholar]
  24. Stjärne L., Astrand P. Relative pre- and postjunctional roles of noradrenaline and adenosine 5'-triphosphate as neurotransmitters of the sympathetic nerves of guinea-pig and mouse vas deferens. Neuroscience. 1985 Mar;14(3):929–946. doi: 10.1016/0306-4522(85)90155-1. [DOI] [PubMed] [Google Scholar]
  25. Su C. Neurogenic release of purine compounds in blood vessels. J Pharmacol Exp Ther. 1975 Oct;195(1):159–166. [PubMed] [Google Scholar]
  26. Vidal M., Hicks P. E., Langer S. Z. Differential effects of alpha-beta-methylene ATP on responses to nerve stimulation in SHR and WKY tail arteries. Naunyn Schmiedebergs Arch Pharmacol. 1986 Apr;332(4):384–390. doi: 10.1007/BF00500092. [DOI] [PubMed] [Google Scholar]
  27. Westfall D. P., Fedan J. S., Colby J., Hogaboom G. K., O'Donnell J. P. Evidence for a contribution by purines to the neurogenic response of the guinea-pig urinary bladder. Eur J Pharmacol. 1983 Mar 4;87(4):415–422. doi: 10.1016/0014-2999(83)90080-8. [DOI] [PubMed] [Google Scholar]
  28. von Kügelgen I., Starke K. Noradrenaline and adenosine triphosphate as co-transmitters of neurogenic vasoconstriction in rabbit mesenteric artery. J Physiol. 1985 Oct;367:435–455. doi: 10.1113/jphysiol.1985.sp015834. [DOI] [PMC free article] [PubMed] [Google Scholar]

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