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. 1990 Feb;99(2):389–395. doi: 10.1111/j.1476-5381.1990.tb14714.x

Enhancement by neuropeptide Y (NPY) of the dihydropyridine-sensitive component of the response to alpha 1-adrenoceptor stimulation in rat isolated mesenteric arterioles.

R Andriantsitohaina 1, J C Stoclet 1
PMCID: PMC1917373  PMID: 1970270

Abstract

1. The mechanism by which neuropeptide Y (NPY) potentiates the vasoconstriction induced by alpha 1-adrenoceptor agonists was investigated in 3rd generation mesenteric arterioles of the rat. 2. At a maximally active concentration, nitrendipine (10(-6) M) displaced to the right the concentration-response curves to noradrenaline (pD2 decreased from 6.2 +/- 0.06 to 5.7 +/- 0.03) and phenylephrine (pD2 decreased from 5.6 +/- 0.03 to 5.3 +/- 0.03). Diltiazem (10(-5) M) also shifted to the right the concentration-response curve to phenylephrine (pD2 decreased from 6.0 +/- 0.06 to 5.5 +/- 0.04). In addition, the maximal response to phenylephrine was significantly decreased in the presence of either nitrendipine or diltiazem. 3. In the absence of a calcium channel blocking agent, NPY (100 nM) produced a leftward shift of the concentration-response curves to noradrenaline (pD2 increased from 6.2 +/- 0.06 to 6.5 +/- 0.05) and phenylephrine (pD2 increased from 5.6 +/- 0.03 to 6.0 +/- 0.06 and from 6.0 +/- 0.06 to 6.3 +/- 0.11). In the presence of either nitrendipine (10(-6) M) or diltiazem (10(-5) M), NPY (100 nM) did not alter the concentration-response curves to either noradrenaline or phenylephrine. 4. NPY was added to arterioles brought to the same level of tension (40% of the maximal contraction) either by phenylephrine alone (1.5 x 10(-6) M) or by a higher concentration of phenylephrine (3 x 10(-6) M) followed by the addition of prazosin (1.3 x 10(-9) M; a concentration at which it partially blocks alpha 1-adrenoceptors).(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Abel P. W., Han C. Effects of neuropeptide Y on contraction, relaxation, and membrane potential of rabbit cerebral arteries. J Cardiovasc Pharmacol. 1989 Jan;13(1):52–63. [PubMed] [Google Scholar]
  2. Agnati L. F., Fuxe K., Benfenati F., Battistini N., Härfstrand A., Tatemoto K., Hökfelt T., Mutt V. Neuropeptide Y in vitro selectivity increases the number of alpha 2-adrenergic binding sites in membranes of the medulla oblongata of the rat. Acta Physiol Scand. 1983 Jul;118(3):293–295. doi: 10.1111/j.1748-1716.1983.tb07273.x. [DOI] [PubMed] [Google Scholar]
  3. Brayden J. E., Conway M. A. Neuropeptide Y and vasoactive intestinal polypeptide in cerebral arteries of the rat: relationships between innervation pattern and mechanical response. Regul Pept. 1988 Aug;22(3):253–265. doi: 10.1016/0167-0115(88)90038-9. [DOI] [PubMed] [Google Scholar]
  4. Cauvin C., Loutzenhiser R., Van Breemen C. Mechanisms of calcium antagonist-induced vasodilation. Annu Rev Pharmacol Toxicol. 1983;23:373–396. doi: 10.1146/annurev.pa.23.040183.002105. [DOI] [PubMed] [Google Scholar]
  5. Cauvin C., Malik S. Induction of Ca++ influx and intracellular Ca++ release in isolated rat aorta and mesenteric resistance vessels by norepinephrine activation of alpha-1 receptors. J Pharmacol Exp Ther. 1984 Aug;230(2):413–418. [PubMed] [Google Scholar]
  6. Dahlöf C., Dahlöf P., Lundberg J. M. Neuropeptide Y (NPY): enhancement of blood pressure increase upon alpha-adrenoceptor activation and direct pressor effects in pithed rats. Eur J Pharmacol. 1985 Feb 26;109(2):289–292. doi: 10.1016/0014-2999(85)90433-9. [DOI] [PubMed] [Google Scholar]
  7. Edvinsson L. Characterization of the contractile effect of neuropeptide Y in feline cerebral arteries. Acta Physiol Scand. 1985 Sep;125(1):33–41. doi: 10.1111/j.1748-1716.1985.tb07690.x. [DOI] [PubMed] [Google Scholar]
  8. Edvinsson L., Ekblad E., Håkanson R., Wahlestedt C. Neuropeptide Y potentiates the effect of various vasoconstrictor agents on rabbit blood vessels. Br J Pharmacol. 1984 Oct;83(2):519–525. doi: 10.1111/j.1476-5381.1984.tb16516.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Edvinsson L., Emson P., McCulloch J., Tatemoto K., Uddman R. Neuropeptide Y: immunocytochemical localization to and effect upon feline pial arteries and veins in vitro and in situ. Acta Physiol Scand. 1984 Oct;122(2):155–163. doi: 10.1111/j.1748-1716.1984.tb07493.x. [DOI] [PubMed] [Google Scholar]
  10. Ekblad E., Edvinsson L., Wahlestedt C., Uddman R., Håkanson R., Sundler F. Neuropeptide Y co-exists and co-operates with noradrenaline in perivascular nerve fibers. Regul Pept. 1984 Apr;8(3):225–235. doi: 10.1016/0167-0115(84)90064-8. [DOI] [PubMed] [Google Scholar]
  11. Exton J. H. Mechanisms of action of calcium-mobilizing agonists: some variations on a young theme. FASEB J. 1988 Aug;2(11):2670–2676. doi: 10.1096/fasebj.2.11.2456243. [DOI] [PubMed] [Google Scholar]
  12. Fredholm B. B., Jansen I., Edvinsson L. Neuropeptide Y is a potent inhibitor of cyclic AMP accumulation in feline cerebral blood vessels. Acta Physiol Scand. 1985 Jul;124(3):467–469. doi: 10.1111/j.1748-1716.1985.tb07683.x. [DOI] [PubMed] [Google Scholar]
  13. Glover W. E. Increased sensitivity of rabbit ear artery to noradrenaline following perivascular nerve stimulation may be a response to neuropeptide Y released as cotransmitter. Clin Exp Pharmacol Physiol. 1985 May-Jun;12(3):227–230. doi: 10.1111/j.1440-1681.1985.tb02636.x. [DOI] [PubMed] [Google Scholar]
  14. Gray T. S., Morley J. E. Neuropeptide Y: anatomical distribution and possible function in mammalian nervous system. Life Sci. 1986 Feb 3;38(5):389–401. doi: 10.1016/0024-3205(86)90061-5. [DOI] [PubMed] [Google Scholar]
  15. Han C., Abel P. W., Minneman K. P. Alpha 1-adrenoceptor subtypes linked to different mechanisms for increasing intracellular Ca2+ in smooth muscle. Nature. 1987 Sep 24;329(6137):333–335. doi: 10.1038/329333a0. [DOI] [PubMed] [Google Scholar]
  16. Han C., Abel P. W. Neuropeptide Y potentiates contraction and inhibits relaxation of rabbit coronary arteries. J Cardiovasc Pharmacol. 1987 Jun;9(6):675–681. doi: 10.1097/00005344-198706000-00006. [DOI] [PubMed] [Google Scholar]
  17. Hanko J. H., Törnebrandt K., Hardebo J. E., Kåhrström J., Nobin A., Owman C. Neuropeptide Y induces and modulates vasoconstriction in intracranial and peripheral vessels of animals and man. J Auton Pharmacol. 1986 Jun;6(2):117–124. doi: 10.1111/j.1474-8673.1986.tb00638.x. [DOI] [PubMed] [Google Scholar]
  18. Hassid A. Atriopeptin II decreases cytosolic free Ca in cultured vascular smooth muscle cells. Am J Physiol. 1986 Nov;251(5 Pt 1):C681–C686. doi: 10.1152/ajpcell.1986.251.5.C681. [DOI] [PubMed] [Google Scholar]
  19. Hinson J., Rauh C., Coupet J. Neuropeptide Y stimulates inositol phospholipid hydrolysis in rat brain miniprisms. Brain Res. 1988 Apr 19;446(2):379–382. doi: 10.1016/0006-8993(88)90898-0. [DOI] [PubMed] [Google Scholar]
  20. Häggblad J., Fredholm B. B. Adenosine and neuropeptide Y enhance alpha 1-adrenoceptor-induced accumulation of inositol phosphates and attenuate forskolin-induced accumulation of cyclic AMP in rat vas deferens. Neurosci Lett. 1987 Nov 23;82(2):211–216. doi: 10.1016/0304-3940(87)90132-7. [DOI] [PubMed] [Google Scholar]
  21. Juan H., Sametz W., Saria A., Pöch G. Eicosapentaenoic acid inhibits vasoconstrictor- and noradrenaline-potentiating effects of neuropeptide Y in the isolated rabbit ear. J Auton Nerv Syst. 1988 Apr;22(3):237–242. doi: 10.1016/0165-1838(88)90112-9. [DOI] [PubMed] [Google Scholar]
  22. Julou G., Freslon J. L. Effects of calcium entry blockers on Ca2+-induced contraction of depolarized and noradrenaline-exposed rat resistance vessels. Eur J Pharmacol. 1986 Oct 7;129(3):261–270. doi: 10.1016/0014-2999(86)90436-x. [DOI] [PubMed] [Google Scholar]
  23. Lundberg J. M., Tatemoto K. Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistant to alpha-adrenoceptor blockade. Acta Physiol Scand. 1982 Dec;116(4):393–402. doi: 10.1111/j.1748-1716.1982.tb07157.x. [DOI] [PubMed] [Google Scholar]
  24. Lundberg J. M., Terenius L., Hökfelt T., Martling C. R., Tatemoto K., Mutt V., Polak J., Bloom S., Goldstein M. Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic function. Acta Physiol Scand. 1982 Dec;116(4):477–480. doi: 10.1111/j.1748-1716.1982.tb07171.x. [DOI] [PubMed] [Google Scholar]
  25. Morgan J. P., Morgan K. G. Alteration of cytoplasmic ionized calcium levels in smooth muscle by vasodilators in the ferret. J Physiol. 1984 Dec;357:539–551. doi: 10.1113/jphysiol.1984.sp015516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Mulvany M. J., Halpern W. Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circ Res. 1977 Jul;41(1):19–26. doi: 10.1161/01.res.41.1.19. [DOI] [PubMed] [Google Scholar]
  27. Mulvany M. J., Nilsson H., Flatman J. A. Role of membrane potential in the response of rat small mesenteric arteries to exogenous noradrenaline stimulation. J Physiol. 1982 Nov;332:363–373. doi: 10.1113/jphysiol.1982.sp014418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Neild T. O. Actions of neuropeptide Y on innervated and denervated rat tail arteries. J Physiol. 1987 May;386:19–30. doi: 10.1113/jphysiol.1987.sp016519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Nichols A. J., Ruffolo R. R., Jr The relationship of alpha-adrenoceptor reserve and agonist intrinsic efficacy to calcium utilization in the vasculature. Trends Pharmacol Sci. 1988 Jul;9(7):236–241. doi: 10.1016/0165-6147(88)90152-6. [DOI] [PubMed] [Google Scholar]
  30. O'Donohue T. L., Chronwall B. M., Pruss R. M., Mezey E., Kiss J. Z., Eiden L. E., Massari V. J., Tessel R. E., Pickel V. M., DiMaggio D. A. Neuropeptide Y and peptide YY neuronal and endocrine systems. Peptides. 1985 Jul-Aug;6(4):755–768. doi: 10.1016/0196-9781(85)90180-9. [DOI] [PubMed] [Google Scholar]
  31. Paglin S., Takuwa Y., Kamm K. E., Stull J. T., Gavras H., Rasmussen H. Atrial natriuretic peptide inhibits the agonist-induced increase in extent of myosin light chain phosphorylation in aortic smooth muscle. J Biol Chem. 1988 Sep 15;263(26):13117–13120. [PubMed] [Google Scholar]
  32. Potter E. K. Neuropeptide Y as an autonomic neurotransmitter. Pharmacol Ther. 1988;37(2):251–273. doi: 10.1016/0163-7258(88)90028-9. [DOI] [PubMed] [Google Scholar]
  33. Reynolds E. E., Yokota S. Neuropeptide Y receptor-effector coupling mechanisms in cultured vascular smooth muscle cells. Biochem Biophys Res Commun. 1988 Mar 15;151(2):919–925. doi: 10.1016/s0006-291x(88)80369-3. [DOI] [PubMed] [Google Scholar]
  34. Saida K., van Breemen C. Mechanism of Ca++ antagonist-induced vasodilation. Intracellular actions. Circ Res. 1983 Feb;52(2):137–142. doi: 10.1161/01.res.52.2.137. [DOI] [PubMed] [Google Scholar]
  35. Seamon K. B., Daly J. W. Forskolin: its biological and chemical properties. Adv Cyclic Nucleotide Protein Phosphorylation Res. 1986;20:1–150. [PubMed] [Google Scholar]
  36. Suzuki Y., Shibuya M., Ikegaki I., Satoh S., Takayasu M., Asano T. Effects of neuropeptide Y on canine cerebral circulation. Eur J Pharmacol. 1988 Feb 9;146(2-3):271–277. doi: 10.1016/0014-2999(88)90302-0. [DOI] [PubMed] [Google Scholar]
  37. Tremblay J., Gerzer R., Hamet P. Cyclic GMP in cell function. Adv Second Messenger Phosphoprotein Res. 1988;22:319–383. [PubMed] [Google Scholar]
  38. Wahlestedt C., Edvinsson L., Ekblad E., Håkanson R. Neuropeptide Y potentiates noradrenaline-evoked vasoconstriction: mode of action. J Pharmacol Exp Ther. 1985 Sep;234(3):735–741. [PubMed] [Google Scholar]
  39. Wahlestedt C., Yanaihara N., Håkanson R. Evidence for different pre-and post-junctional receptors for neuropeptide Y and related peptides. Regul Pept. 1986 Feb;13(3-4):307–318. doi: 10.1016/0167-0115(86)90048-0. [DOI] [PubMed] [Google Scholar]
  40. Zukowska-Grojec Z., Haass M., Bayorh M. A. Neuropeptide Y and peptide YY mediate nonadrenergic vasoconstriction and modulate sympathetic responses in rats. Regul Pept. 1986 Sep;15(2):99–110. doi: 10.1016/0167-0115(86)90080-7. [DOI] [PubMed] [Google Scholar]
  41. Zukowska-Grojec Z., Marks E. S., Haass M. Neuropeptide Y is a potent vasoconstrictor and a cardiodepressant in rat. Am J Physiol. 1987 Nov;253(5 Pt 2):H1234–H1239. doi: 10.1152/ajpheart.1987.253.5.H1234. [DOI] [PubMed] [Google Scholar]

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