Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1990 Feb;99(2):267–272. doi: 10.1111/j.1476-5381.1990.tb14692.x

Eosinophil accumulation in pulmonary airways of guinea-pigs induced by exposure to an aerosol of platelet-activating factor: effect of anti-asthma drugs.

S Sanjar 1, S Aoki 1, K Boubekeur 1, I D Chapman 1, D Smith 1, M A Kings 1, J Morley 1
PMCID: PMC1917376  PMID: 2328394

Abstract

1. Exposure of guinea-pigs to aerosols of platelet activating factor (PAF) (0.01 to 100 micrograms ml-1) induced a dose-dependent increased incidence of eosinophils in bronchoalveolar lavage fluid (BAL) at 48 h. Total leucocyte numbers and the percentages of lymphocytes and neutrophils were unchanged in BAL fluid. 2. Increased numbers of eosinophils were detected in BAL 1 h after exposure to PAF but eosinophilia was not maximal until 48 h. One week after exposure to PAF, the percentage of eosinophils in BAL was within the normal range. 3. Depletion of circulating platelets or neutrophils by intravenous injection of specific antisera did not modify accumulation of eosinophils in the airway lumen following inhalation of PAF (10 micrograms ml-1). 4. PAF-induced pulmonary airway eosinophil accumulation was inhibited by treatment with SDZ 64-412, a selective PAF-antagonist, whether the compound was administered before, or 30 min after, inhalation of PAF. 5. Pulmonary airway eosinophil accumulation due to inhaled PAF (10 micrograms ml-1) was inhibited by prior treatment with aminophylline, cromoglycate, ketotifen, dexamethasone and AH 21-132. 6. Pulmonary airway eosinophil accumulation due to inhaled PAF (10 micrograms ml-1) was not inhibited by prior treatment with indomethacin, salbutamol or mepyramine.

Full text

PDF
271

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archer C. B., Page C. P., Morley J., MacDonald D. M. Accumulation of inflammatory cells in response to intracutaneous platelet activating factor (Paf-acether) in man. Br J Dermatol. 1985 Mar;112(3):285–290. doi: 10.1111/j.1365-2133.1985.tb04855.x. [DOI] [PubMed] [Google Scholar]
  2. Arnoux B., Denjean A., Page C. P., Nolibe D., Morley J., Benveniste J. Accumulation of platelets and eosinophils in baboon lung after paf-acether challenge. Inhibition by ketotifen. Am Rev Respir Dis. 1988 Apr;137(4):855–860. doi: 10.1164/ajrccm/137.4.855. [DOI] [PubMed] [Google Scholar]
  3. Arnoux B., Duval D., Benveniste J. Release of platelet-activating factor (PAF-acether) from alveolar macrophages by the calcium ionophore A23187 and phagocytosis. Eur J Clin Invest. 1980 Dec;10(6):437–441. doi: 10.1111/j.1365-2362.1980.tb02082.x. [DOI] [PubMed] [Google Scholar]
  4. Benveniste J., Henson P. M., Cochrane C. G. Leukocyte-dependent histamine release from rabbit platelets. The role of IgE, basophils, and a platelet-activating factor. J Exp Med. 1972 Dec 1;136(6):1356–1377. doi: 10.1084/jem.136.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bourgain R. H., Maes L., Andries R., Braquet P. Thrombus induction by endogenic paf-acether and its inhibition by Ginkgo Biloba extracts in the guinea pig. Prostaglandins. 1986 Jul;32(1):142–144. doi: 10.1016/0090-6980(86)90157-7. [DOI] [PubMed] [Google Scholar]
  6. Braquet P., Touqui L., Shen T. Y., Vargaftig B. B. Perspectives in platelet-activating factor research. Pharmacol Rev. 1987 Jun;39(2):97–145. [PubMed] [Google Scholar]
  7. Broder I., Rogers S., Chamberlain D. W., Milne E. N. Model of allergic bronchoconstriction in the guinea pig. I. Characteristics of the system. Clin Immunol Immunopathol. 1978 Jan;9(1):1–15. doi: 10.1016/0090-1229(78)90114-9. [DOI] [PubMed] [Google Scholar]
  8. Brown H. M., Storey G., Jackson F. A. Beclomethasone dipropionate aerosol in long-term treatment of perennial and seasonal asthma in children and adults: a report of five-and-half years' experience in 600 asthmatic patients. Br J Clin Pharmacol. 1977;4 (Suppl 3):259S–267S. doi: 10.1111/j.1365-2125.1977.tb04517.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Camussi G., Pawlowski I., Tetta C., Roffinello C., Alberton M., Brentjens J., Andres G. Acute lung inflammation induced in the rabbit by local instillation of 1-0-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine or of native platelet-activating factor. Am J Pathol. 1983 Jul;112(1):78–88. [PMC free article] [PubMed] [Google Scholar]
  10. Dahl R., Venge P., Olsson I. Variations of blood eosinophils and eosinophil cationic protein in serum in patients with bronchial asthma. Studies during inhalation challenge test. Allergy. 1978 Aug;33(4):211–215. doi: 10.1111/j.1398-9995.1978.tb01536.x. [DOI] [PubMed] [Google Scholar]
  11. Dahl R., Venge P. Role of the eosinophil in bronchial asthma. Eur J Respir Dis Suppl. 1982;122:23–28. [PubMed] [Google Scholar]
  12. De Monchy J. G., Kauffman H. F., Venge P., Koëter G. H., Jansen H. M., Sluiter H. J., De Vries K. Bronchoalveolar eosinophilia during allergen-induced late asthmatic reactions. Am Rev Respir Dis. 1985 Mar;131(3):373–376. doi: 10.1164/arrd.1985.131.3.373. [DOI] [PubMed] [Google Scholar]
  13. Dewar A., Archer C. B., Paul W., Page C. P., MacDonald D. M., Morley J. Cutaneous and pulmonary histopathological responses to platelet activating factor (Paf-acether) in the guinea-pig. J Pathol. 1984 Sep;144(1):25–34. doi: 10.1002/path.1711440104. [DOI] [PubMed] [Google Scholar]
  14. Diaz P., Galleguillos F. R., Gonzalez M. C., Pantin C. F., Kay A. B. Bronchoalveolar lavage in asthma: the effect of disodium cromoglycate (cromolyn) on leukocyte counts, immunoglobulins, and complement. J Allergy Clin Immunol. 1984 Jul;74(1):41–48. doi: 10.1016/0091-6749(84)90085-x. [DOI] [PubMed] [Google Scholar]
  15. Fitzgerald M. F., Moncada S., Parente L. The anaphylactic release of platelet-activating factor from perfused guinea-pig lungs. Br J Pharmacol. 1986 May;88(1):149–153. doi: 10.1111/j.1476-5381.1986.tb09481.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Frigas E., Gleich G. J. The eosinophil and the pathophysiology of asthma. J Allergy Clin Immunol. 1986 Apr;77(4):527–537. doi: 10.1016/0091-6749(86)90341-6. [DOI] [PubMed] [Google Scholar]
  17. Frigas E., Loegering D. A., Solley G. O., Farrow G. M., Gleich G. J. Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma. Mayo Clin Proc. 1981 Jun;56(6):345–353. [PubMed] [Google Scholar]
  18. Fügner A. Formation of oedema and accumulation of eosinophils in the guinea pig lung. Inhibition by inhaled beta-stimulants. Int Arch Allergy Appl Immunol. 1989;88(1-2):225–227. doi: 10.1159/000234792. [DOI] [PubMed] [Google Scholar]
  19. Göbel P. The protective effect of ketotifen in bronchial asthma. J Int Med Res. 1978;6(2):78–85. [PubMed] [Google Scholar]
  20. Handley D. A., Van Valen R. G., Melden M. K., Houlihan W. J., Saunders R. N. Biological effects of the orally active platelet activating factor receptor antagonist SDZ 64-412. J Pharmacol Exp Ther. 1988 Nov;247(2):617–623. [PubMed] [Google Scholar]
  21. Henocq E., Vargaftig B. B. Accumulation of eosinophils in response to intracutaneous PAF-acether and allergens in man. Lancet. 1986 Jun 14;1(8494):1378–1379. doi: 10.1016/s0140-6736(86)91683-1. [DOI] [PubMed] [Google Scholar]
  22. Horn B. R., Robin E. D., Theodore J., Van Kessel A. Total eosinophil counts in the management of bronchial asthma. N Engl J Med. 1975 May 29;292(22):1152–1155. doi: 10.1056/NEJM197505292922204. [DOI] [PubMed] [Google Scholar]
  23. Hutson P. A., Holgate S. T., Church M. K. The effect of cromolyn sodium and albuterol on early and late phase bronchoconstriction and airway leukocyte infiltration after allergen challenge of nonanesthetized guinea pigs. Am Rev Respir Dis. 1988 Nov;138(5):1157–1163. doi: 10.1164/ajrccm/138.5.1157. [DOI] [PubMed] [Google Scholar]
  24. Lartigue-Mattei C., Godeneche D., Chabard J. L., Petit J., Berger J. A. Pharmacokinetic study of 3H-labelled PAF-acether II. Comparison with 3H-labelled lyso-PAF-acether after intravenous administration in the rabbit and protein binding. Agents Actions. 1984 Dec;15(5-6):643–648. doi: 10.1007/BF01966786. [DOI] [PubMed] [Google Scholar]
  25. Lee T., Lenihan D. J., Malone B., Roddy L. L., Wasserman S. I. Increased biosynthesis of platelet-activating factor in activated human eosinophils. J Biol Chem. 1984 May 10;259(9):5526–5530. [PubMed] [Google Scholar]
  26. Lefort J., Rotilio D., Vargaftig B. B. The platelet-independent release of thromboxane A2 by Paf-acether from guinea-pig lungs involves mechanisms distinct from those for leukotriene. Br J Pharmacol. 1984 Jul;82(3):565–575. doi: 10.1111/j.1476-5381.1984.tb10795.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Lellouch-Tubiana A., Lefort J., Simon M. T., Pfister A., Vargaftig B. B. Eosinophil recruitment into guinea pig lungs after PAF-acether and allergen administration. Modulation by prostacyclin, platelet depletion, and selective antagonists. Am Rev Respir Dis. 1988 Apr;137(4):948–954. doi: 10.1164/ajrccm/137.4.948. [DOI] [PubMed] [Google Scholar]
  28. Nakamura T., Morita Y., Kuriyama M., Ishihara K., Ito K., Miyamoto T. Platelet-activating factor in late asthmatic response. Int Arch Allergy Appl Immunol. 1987;82(1):57–61. doi: 10.1159/000234290. [DOI] [PubMed] [Google Scholar]
  29. Richerson H. B. Acute experimental hypersensitivity pneumonitis in the guinea pig. J Lab Clin Med. 1972 May;79(5):745–757. [PubMed] [Google Scholar]
  30. Spicer B. A., Laycock S. M., Smith H. The effects of drugs on leucocyte changes following the injection of antigen into the peritoneal cavities of actively sensitised rats. Agents Actions. 1986 Mar;17(5-6):498–505. doi: 10.1007/BF01965521. [DOI] [PubMed] [Google Scholar]
  31. Thorne K. J., Richardson B. A., Butterworth A. E., Hay I., Higenbottam T. W. Effect of drugs used in the treatment of asthma on the production of eosinophil-activating factor by monocytes. Int Arch Allergy Appl Immunol. 1988;85(2):257–259. doi: 10.1159/000234513. [DOI] [PubMed] [Google Scholar]
  32. Vargaftig B. B., Lefort J., Chignard M., Benveniste J. Platelet-activating factor induces a platelet-dependent bronchoconstriction unrelated to the formation of prostaglandin derivatives. Eur J Pharmacol. 1980 Jul 25;65(2-3):185–192. doi: 10.1016/0014-2999(80)90391-x. [DOI] [PubMed] [Google Scholar]
  33. Verhagen J., Bruynzeel P. L., Koedam J. A., Wassink G. A., de Boer M., Terpstra G. K., Kreukniet J., Veldink G. A., Vliegenthart J. F. Specific leukotriene formation by purified human eosinophils and neutrophils. FEBS Lett. 1984 Mar 12;168(1):23–28. doi: 10.1016/0014-5793(84)80199-4. [DOI] [PubMed] [Google Scholar]
  34. Wardlaw A. J., Moqbel R., Cromwell O., Kay A. B. Platelet-activating factor. A potent chemotactic and chemokinetic factor for human eosinophils. J Clin Invest. 1986 Dec;78(6):1701–1706. doi: 10.1172/JCI112765. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES