Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1993 Dec;37(12):2699–2705. doi: 10.1128/aac.37.12.2699

A new tetracycline resistance determinant, Tet H, from Pasteurella multocida specifying active efflux of tetracycline.

L M Hansen 1, L M McMurry 1, S B Levy 1, D C Hirsh 1
PMCID: PMC192782  PMID: 8109938

Abstract

The tetracycline resistance determinant on plasmid pVM111 from an avian strain of Pasteurella multocida mediates tetracycline resistance by a regulated active efflux mechanism. DNA coding for the determinant did not hybridize at high stringency with DNA representing a group of common tetracycline resistance determinants. The DNA sequence, however; revealed a structural gene and a repressor gene which had significant (37 to 64%) sequence similarities with previously described classes of efflux-type tetracycline resistance genes from members of the family Enterobacteriaceae. The new determinant has been assigned to class H.

Full text

PDF
2701

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allard J. D., Bertrand K. P. Sequence of a class E tetracycline resistance gene from Escherichia coli and comparison of related tetracycline efflux proteins. J Bacteriol. 1993 Jul;175(14):4554–4560. doi: 10.1128/jb.175.14.4554-4560.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allard J. D., Gibson M. L., Vu L. H., Nguyen T. T., Bertrand K. P. Nucleotide sequence of class D tetracycline resistance genes from Salmonella ordonez. Mol Gen Genet. 1993 Feb;237(1-2):301–305. doi: 10.1007/BF00282811. [DOI] [PubMed] [Google Scholar]
  3. Aoki T., Takahashi A. Class D tetracycline resistance determinants of R plasmids from the fish pathogens Aeromonas hydrophila, Edwardsiella tarda, and Pasteurella piscicida. Antimicrob Agents Chemother. 1987 Aug;31(8):1278–1280. doi: 10.1128/aac.31.8.1278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Archer G. L., Scott J. Conjugative transfer genes in staphylococcal isolates from the United States. Antimicrob Agents Chemother. 1991 Dec;35(12):2500–2504. doi: 10.1128/aac.35.12.2500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bernardi A., Bernardi F. Complete sequence of pSC101. Nucleic Acids Res. 1984 Dec 21;12(24):9415–9426. doi: 10.1093/nar/12.24.9415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burdett V., Inamine J., Rajagopalan S. Heterogeneity of tetracycline resistance determinants in Streptococcus. J Bacteriol. 1982 Mar;149(3):995–1004. doi: 10.1128/jb.149.3.995-1004.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carpenter T. E., Snipes K. P., Wallis D., McCapes R. Epidemiology and financial impact of fowl cholera in turkeys: a retrospective analysis. Avian Dis. 1988 Jan-Mar;32(1):16–23. [PubMed] [Google Scholar]
  8. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cuchural G. J., Jr, Tally F. P., Jacobus N. V., Aldridge K., Cleary T., Finegold S. M., Hill G., Iannini P., O'Keefe J. P., Pierson C. Susceptibility of the Bacteroides fragilis group in the United States: analysis by site of isolation. Antimicrob Agents Chemother. 1988 May;32(5):717–722. doi: 10.1128/aac.32.5.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Curiale M. S., Levy S. B. Two complementation groups mediate tetracycline resistance determined by Tn10. J Bacteriol. 1982 Jul;151(1):209–215. doi: 10.1128/jb.151.1.209-215.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Guerry P., van Embden J., Falkow S. Molecular nature of two nonconjugative plasmids carrying drug resistance genes. J Bacteriol. 1974 Feb;117(2):619–630. doi: 10.1128/jb.117.2.619-630.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hirsh D. C., Burton G. C., Blenden D. C. The effect of tetracycline upon establishment of Escherichia coli of bovine origin in the enteric tract of man. J Appl Bacteriol. 1974 Sep;37(3):327–333. doi: 10.1111/j.1365-2672.1974.tb00447.x. [DOI] [PubMed] [Google Scholar]
  13. Hirsh D. C., Hansen L. M., Dorfman L. C., Snipes K. P., Carpenter T. E., Hird D. W., McCapes R. H. Resistance to antimicrobial agents and prevalence of R plasmids in Pasteurella multocida from turkeys. Antimicrob Agents Chemother. 1989 May;33(5):670–673. doi: 10.1128/aac.33.5.670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hirsh D. C., Ling G. V., Ruby A. L. Incidence of R-plasmids in fecal flora of healthy household dogs. Antimicrob Agents Chemother. 1980 Mar;17(3):313–315. doi: 10.1128/aac.17.3.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hirsh D. C., Martin L. D., Rhoades K. R. Resistance plasmids of Pasteurella multocida isolated from turkeys. Am J Vet Res. 1985 Jul;46(7):1490–1493. [PubMed] [Google Scholar]
  16. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Levy S. B. Evolution and spread of tetracycline resistance determinants. J Antimicrob Chemother. 1989 Jul;24(1):1–3. doi: 10.1093/jac/24.1.1. [DOI] [PubMed] [Google Scholar]
  18. Marshall B., Morrissey S., Flynn P., Levy S. B. A new tetracycline-resistance determinant, class E, isolated from Enterobacteriaceae. Gene. 1986;50(1-3):111–117. doi: 10.1016/0378-1119(86)90315-x. [DOI] [PubMed] [Google Scholar]
  19. Marshall B., Tachibana C., Levy S. B. Frequency of tetracycline resistance determinant classes among lactose-fermenting coliforms. Antimicrob Agents Chemother. 1983 Dec;24(6):835–840. doi: 10.1128/aac.24.6.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. McMurry L. M., Cullinane J. C., Levy S. B. Transport of the lipophilic analog minocycline differs from that of tetracycline in susceptible and resistant Escherichia coli strains. Antimicrob Agents Chemother. 1982 Nov;22(5):791–799. doi: 10.1128/aac.22.5.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mendez B., Tachibana C., Levy S. B. Heterogeneity of tetracycline resistance determinants. Plasmid. 1980 Mar;3(2):99–108. doi: 10.1016/0147-619x(80)90101-8. [DOI] [PubMed] [Google Scholar]
  22. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Nguyen T. T., Postle K., Bertrand K. P. Sequence homology between the tetracycline-resistance determinants of Tn10 and pBR322. Gene. 1983 Nov;25(1):83–92. doi: 10.1016/0378-1119(83)90170-1. [DOI] [PubMed] [Google Scholar]
  24. Pacer R. E., Spika J. S., Thurmond M. C., Hargrett-Bean N., Potter M. E. Prevalence of Salmonella and multiple antimicrobial-resistant Salmonella in California dairies. J Am Vet Med Assoc. 1989 Jul 1;195(1):59–63. [PubMed] [Google Scholar]
  25. Peden K. W. Revised sequence of the tetracycline-resistance gene of pBR322. Gene. 1983 May-Jun;22(2-3):277–280. doi: 10.1016/0378-1119(83)90112-9. [DOI] [PubMed] [Google Scholar]
  26. Postle K., Nguyen T. T., Bertrand K. P. Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant. Nucleic Acids Res. 1984 Jun 25;12(12):4849–4863. doi: 10.1093/nar/12.12.4849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Reece R. L., Coloe P. J. The resistance to anti-microbial agents of bacteria isolated from pathological conditions of birds in Victoria, 1978 to 1983. Aust Vet J. 1985 Nov;62(11):379–381. doi: 10.1111/j.1751-0813.1985.tb14214.x. [DOI] [PubMed] [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Simoni R. D., Shallenberger M. K. Coupling of energy to active transport of amino acids in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2663–2667. doi: 10.1073/pnas.69.9.2663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tauxe R. V., Puhr N. D., Wells J. G., Hargrett-Bean N., Blake P. A. Antimicrobial resistance of Shigella isolates in the USA: the importance of international travelers. J Infect Dis. 1990 Nov;162(5):1107–1111. doi: 10.1093/infdis/162.5.1107. [DOI] [PubMed] [Google Scholar]
  31. Tovar K., Ernst A., Hillen W. Identification and nucleotide sequence of the class E tet regulatory elements and operator and inducer binding of the encoded purified Tet repressor. Mol Gen Genet. 1988 Dec;215(1):76–80. doi: 10.1007/BF00331306. [DOI] [PubMed] [Google Scholar]
  32. Unger B., Klock G., Hillen W. Nucleotide sequence of the repressor gene of the RA1 tetracycline resistance determinant: structural and functional comparison with three related Tet repressor genes. Nucleic Acids Res. 1984 Oct 25;12(20):7693–7703. doi: 10.1093/nar/12.20.7693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Waters S. H., Rogowsky P., Grinsted J., Altenbuchner J., Schmitt R. The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis. Nucleic Acids Res. 1983 Sep 10;11(17):6089–6105. doi: 10.1093/nar/11.17.6089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Yamaguchi A., Ohmori H., Kaneko-Ohdera M., Nomura T., Sawai T. Delta pH-dependent accumulation of tetracycline in Escherichia coli. Antimicrob Agents Chemother. 1991 Jan;35(1):53–56. doi: 10.1128/aac.35.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Zhao J., Aoki T. Nucleotide sequence analysis of the class G tetracycline resistance determinant from Vibrio anguillarum. Microbiol Immunol. 1992;36(10):1051–1060. doi: 10.1111/j.1348-0421.1992.tb02109.x. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES