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. 1992 Feb;58(2):765–768. doi: 10.1128/aem.58.2.765-768.1992

Suitability of the prfA gene, which encodes a regulator of virulence genes in Listeria monocytogenes, in the identification of pathogenic Listeria spp.

K Wernars 1, K Heuvelman 1, S Notermans 1, E Domann 1, M Leimeister-Wächter 1, T Chakraborty 1
PMCID: PMC195324  PMID: 1610204

Abstract

The pathogenesis of listerial infections is complex and involves a number of virulence factors expressed by virulent Listeria species. We have recently described a regulator gene, prfA, that positively regulates the expression of a number of virulence factors in Listeria monocytogenes. When the prfA gene was used as a DNA probe, we found it to be extremely specific for the pathogenic species L. monocytogenes. No reaction was obtained with strains of all other species of this genus. By using this information, an oligonucleotide primer pair was developed that specifically amplifies the prfA gene in L. monocytogenes strains of all known serotypes.

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Selected References

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  1. Border P. M., Howard J. J., Plastow G. S., Siggens K. W. Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction. Lett Appl Microbiol. 1990 Sep;11(3):158–162. doi: 10.1111/j.1472-765x.1990.tb00149.x. [DOI] [PubMed] [Google Scholar]
  2. Chakraborty T., Goebel W. Recent developments in the study of virulence in Listeria monocytogenes. Curr Top Microbiol Immunol. 1988;138:41–58. [PubMed] [Google Scholar]
  3. Chakraborty T., Leimeister-Wächter M., Domann E., Hartl M., Goebel W., Nichterlein T., Notermans S. Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prfA gene. J Bacteriol. 1992 Jan;174(2):568–574. doi: 10.1128/jb.174.2.568-574.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Datta A. R., Wentz B. A., Hill W. E. Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization. Appl Environ Microbiol. 1987 Sep;53(9):2256–2259. doi: 10.1128/aem.53.9.2256-2259.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Domann E., Leimeister-Wächter M., Goebel W., Chakraborty T. Molecular cloning, sequencing, and identification of a metalloprotease gene from Listeria monocytogenes that is species specific and physically linked to the listeriolysin gene. Infect Immun. 1991 Jan;59(1):65–72. doi: 10.1128/iai.59.1.65-72.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fleming D. W., Cochi S. L., MacDonald K. L., Brondum J., Hayes P. S., Plikaytis B. D., Holmes M. B., Audurier A., Broome C. V., Reingold A. L. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med. 1985 Feb 14;312(7):404–407. doi: 10.1056/NEJM198502143120704. [DOI] [PubMed] [Google Scholar]
  7. Furrer B., Candrian U., Hoefelein C., Luethy J. Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in vitro amplification of haemolysin gene fragments. J Appl Bacteriol. 1991 May;70(5):372–379. doi: 10.1111/j.1365-2672.1991.tb02951.x. [DOI] [PubMed] [Google Scholar]
  8. Gellin B. G., Broome C. V. Listeriosis. JAMA. 1989 Mar 3;261(9):1313–1320. [PubMed] [Google Scholar]
  9. Leimeister-Wächter M., Domann E., Chakraborty T. Detection of a gene encoding a phosphatidylinositol-specific phospholipase C that is co-ordinately expressed with listeriolysin in Listeria monocytogenes. Mol Microbiol. 1991 Feb;5(2):361–366. doi: 10.1111/j.1365-2958.1991.tb02117.x. [DOI] [PubMed] [Google Scholar]
  10. Leimeister-Wächter M., Haffner C., Domann E., Goebel W., Chakraborty T. Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of listeria monocytogenes. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8336–8340. doi: 10.1073/pnas.87.21.8336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Linnan M. J., Mascola L., Lou X. D., Goulet V., May S., Salminen C., Hird D. W., Yonekura M. L., Hayes P., Weaver R. Epidemic listeriosis associated with Mexican-style cheese. N Engl J Med. 1988 Sep 29;319(13):823–828. doi: 10.1056/NEJM198809293191303. [DOI] [PubMed] [Google Scholar]
  12. Notermans S. H., Dufrenne J., Leimeister-Wächter M., Domann E., Chakraborty T. Phosphatidylinositol-specific phospholipase C activity as a marker to distinguish between pathogenic and nonpathogenic Listeria species. Appl Environ Microbiol. 1991 Sep;57(9):2666–2670. doi: 10.1128/aem.57.9.2666-2670.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Notermans S., Chakraborty T., Leimeister-Wächter M., Dufrenne J., Heuvelman K. J., Maas H., Jansen W., Wernars K., Guinee P. Specific gene probe for detection of biotyped and serotyped Listeria strains. Appl Environ Microbiol. 1989 Apr;55(4):902–906. doi: 10.1128/aem.55.4.902-906.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rocourt J., Schrettenbrunner A., Seeliger H. P. Différenciation biochimique des groupes génomiques de Listeria monocytogenes (sensu lato). Ann Microbiol (Paris) 1983 Jan-Feb;134A(1):65–71. [PubMed] [Google Scholar]
  15. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  16. Schlech W. F., 3rd, Lavigne P. M., Bortolussi R. A., Allen A. C., Haldane E. V., Wort A. J., Hightower A. W., Johnson S. E., King S. H., Nicholls E. S. Epidemic listeriosis--evidence for transmission by food. N Engl J Med. 1983 Jan 27;308(4):203–206. doi: 10.1056/NEJM198301273080407. [DOI] [PubMed] [Google Scholar]
  17. Wernars K., Heuvelman C. J., Chakraborty T., Notermans S. H. Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese. J Appl Bacteriol. 1991 Feb;70(2):121–126. doi: 10.1111/j.1365-2672.1991.tb04437.x. [DOI] [PubMed] [Google Scholar]
  18. van Netten P., Perales I., van de Moosdijk A., Curtis G. D., Mossel D. A. Liquid and solid selective differential media for the detection and enumeration of L. monocytogenes and other Listeria spp. Int J Food Microbiol. 1989 Jul;8(4):299–316. doi: 10.1016/0168-1605(89)90001-9. [DOI] [PubMed] [Google Scholar]

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