Abstract
A mutant, which has low Δ5-desaturase activity, of an arachidonic acid-producing fungus, Mortierella alpina 1S-4, was shown to be a novel potent producer of dihomo-γ-linolenic acid (DHGA). On submerged culture under optimal conditions for 6 days at 28°C in a 10-liter fermentor, the mutant produced 3.2 g of DHGA per liter of culture broth (123 mg/g of dry mycelia), which accounted for 23.4% of the total mycelial fatty acids. Mycelial arachidonic acid amounted to only 19 mg/g of dry mycelia (0.5 g/liter of culture broth), which accounted for 3.7% of the total mycelial fatty acids. The other major mycelial fatty acids were palmitic acid (11.0%), stearic acid (12.8%), oleic acid (22.7%), linoleic acid (8.9%), γ-linolenic acid (6.5%), and lignoceric acid (7.8%). More than 97 mol% of the DHGA produced was found in the triglyceride fraction irrespective of the growth temperature employed (12 to 28°C).
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- FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
- HULANICKA D., ERWIN J., BLOCH K. LIPID METABOLISM OF EUGLENA GRACILIS. J Biol Chem. 1964 Sep;239:2778–2787. [PubMed] [Google Scholar]
- Pugh E. L., Kates M. Direct desaturation of eicosatrienoyl lecithin to arachidonoyl lecithin by rat liver microsomes. J Biol Chem. 1977 Jan 10;252(1):68–73. [PubMed] [Google Scholar]
- Shimizu S., Akimoto K., Shinmen Y., Kawashima H., Sugano M., Yamada H. Sesamin is a potent and specific inhibitor of delta 5 desaturase in polyunsaturated fatty acid biosynthesis. Lipids. 1991 Jul;26(7):512–516. doi: 10.1007/BF02536595. [DOI] [PubMed] [Google Scholar]
- Shimizu S., Shinmen Y., Kawashima H., Akimoto K., Yamada H. Fungal mycelia as a novel source of eicosapentaenoic acid. Activation of enzyme(s) involved in eicosapentaenoic acid production at low temperature. Biochem Biophys Res Commun. 1988 Jan 15;150(1):335–341. doi: 10.1016/0006-291x(88)90525-6. [DOI] [PubMed] [Google Scholar]