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. 1993 Dec;59(12):4051–4055. doi: 10.1128/aem.59.12.4051-4055.1993

Surface Chemistry of Thiobacillus ferrooxidans Relevant to Adhesion on Mineral Surfaces

Preston Devasia 1, K A Natarajan 1,*, D N Sathyanarayana 1, G Ramananda Rao 1
PMCID: PMC195866  PMID: 16349107

Abstract

Thiobacillus ferrooxidans cells grown on sulfur, pyrite, and chalcopyrite exhibit greater hydrophobicity than ferrous ion-grown cells. The isoelectric points of sulfur-, pyrite-, and chalcopyrite-grown cells were observed to be at a pH higher than that for ferrous ion-grown cells. Microbe-mineral interactions result in change in the surface chemistry of the organism as well as that of the minerals with which it has interacted. Sulfur, pyrite, and chalcopyrite after interaction with T. ferrooxidans exhibited a significant shift in their isoelectric points from the initial values exhibited by uninteracted minerals. With antibodies raised against sulfur-grown T. ferrooxidans, pyrite- and chalcopyrite-grown cells showed immunoreactivity, whereas ferrous ion-grown cells failed to do so. Fourier transform infrared spectroscopy of sulfur-grown cells suggested that a proteinaceous new cell surface appendage synthesized in mineral-grown cells brings about adhesion to the solid mineral substrates. Such an appendage was found to be absent in ferrous ion-grown cells as it is not required during growth in liquid substrates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartlett W. C., Noelle R. J. A cell surface ELISA to detect interleukin-4-induced class II MHC expression on murine B cells. J Immunol Methods. 1987 Dec 4;105(1):79–85. doi: 10.1016/0022-1759(87)90416-9. [DOI] [PubMed] [Google Scholar]
  2. Brierley C. L. Bacterial leaching. CRC Crit Rev Microbiol. 1978;6(3):207–26I. doi: 10.3109/10408417809090623. [DOI] [PubMed] [Google Scholar]
  3. Espejo R. T., Romero P. Growth of Thiobacillus ferrooxidans on Elemental Sulfur. Appl Environ Microbiol. 1987 Aug;53(8):1907–1912. doi: 10.1128/aem.53.8.1907-1912.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hutchins S. R., Davidson M. S., Brierley J. A., Brierley C. L. Microorganisms in reclamation of metals. Annu Rev Microbiol. 1986;40:311–336. doi: 10.1146/annurev.mi.40.100186.001523. [DOI] [PubMed] [Google Scholar]
  5. SILVERMAN M. P., LUNDGREN D. G. Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J Bacteriol. 1959 May;77(5):642–647. doi: 10.1128/jb.77.5.642-647.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Smyth C. J., Jonsson P., Olsson E., Soderlind O., Rosengren J., Hjertén S., Wadström T. Differences in hydrophobic surface characteristics of porcine enteropathogenic Escherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography. Infect Immun. 1978 Nov;22(2):462–472. doi: 10.1128/iai.22.2.462-472.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. van Loosdrecht M. C., Lyklema J., Norde W., Schraa G., Zehnder A. J. Electrophoretic mobility and hydrophobicity as a measured to predict the initial steps of bacterial adhesion. Appl Environ Microbiol. 1987 Aug;53(8):1898–1901. doi: 10.1128/aem.53.8.1898-1901.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. van Loosdrecht M. C., Lyklema J., Norde W., Zehnder A. J. Influence of interfaces on microbial activity. Microbiol Rev. 1990 Mar;54(1):75–87. doi: 10.1128/mr.54.1.75-87.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. van der Mei H. C., Léonard A. J., Weerkamp A. H., Rouxhet P. G., Busscher H. J. Surface properties of Streptococcus salivarius HB and nonfibrillar mutants: measurement of zeta potential and elemental composition with X-ray photoelectron spectroscopy. J Bacteriol. 1988 Jun;170(6):2462–2466. doi: 10.1128/jb.170.6.2462-2466.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. van der Mei H. C., Noordmans J., Busscher H. J. Molecular surface characterization of oral streptococci by Fourier transform infrared spectroscopy. Biochim Biophys Acta. 1989 Jun 27;991(3):395–398. doi: 10.1016/0304-4165(89)90063-9. [DOI] [PubMed] [Google Scholar]

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