Abstract
Vibrio sp. strain ABE-1 was grown in a medium that contained as its stable isotope tracer either [2,2-2H2]cis-9-hexadecenoic or [2,2-2H2]trans-9-hexadecenoic acid. Gas chromatographic-mass spectrometric analysis of the cis-9-hexadecenoic and trans-9-hexadecenoic acid fractions from the cells revealed the formation of an intracellularly isomerized 2,2-2H2-fatty acid which differed from the tracer only in the geometrical configuration of the double bond. This observation shows that cis-trans isomerization without a shift in double-bond position between these two geometric hexadecenoic acid isomers can occur in the cells.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- Morita N., Gotoh M., Okajima N., Okuyama H., Hayashi H., Higashi S., Murata N. Both the anaerobic pathway and aerobic desaturation are involved in the synthesis of unsaturated fatty acids in Vibrio sp. strain ABE-1. FEBS Lett. 1992 Feb 3;297(1-2):9–12. doi: 10.1016/0014-5793(92)80316-9. [DOI] [PubMed] [Google Scholar]
- Morita N., Okajima N., Gotoh M., Hayashi H., Okuyama H., Sasaki S. Synthesis in vitro of very long chain fatty acids in Vibrio sp. strain ABE-1. Arch Microbiol. 1992;157(3):223–228. doi: 10.1007/BF00245154. [DOI] [PubMed] [Google Scholar]
- Okuyama H., Okajima N., Sasaki S., Higashi S., Murata N. The cis/trans isomerization of the double bond of a fatty acid as a strategy for adaptation to changes in ambient temperature in the psychrophilic bacterium, Vibrio sp. strain ABE-1. Biochim Biophys Acta. 1991 Jun 19;1084(1):13–20. doi: 10.1016/0005-2760(91)90049-n. [DOI] [PubMed] [Google Scholar]
- Okuyama H., Sasaki S., Higashi S., Murata N. A trans-unsaturated fatty acid in a psychrophilic bacterium, Vibrio sp. strain ABE-1. J Bacteriol. 1990 Jun;172(6):3515–3518. doi: 10.1128/jb.172.6.3515-3518.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Omura S. Cerulenin. Methods Enzymol. 1981;72:520–532. [PubMed] [Google Scholar]
- Shibahara A., Yamamoto K., Takeoka M., Kinoshita A., Kajimoto G., Nakayama T., Noda M. Novel pathways of oleic and cis-vaccenic acid biosynthesis by an enzymatic double-bond shifting reaction in higher plants. FEBS Lett. 1990 May 21;264(2):228–230. doi: 10.1016/0014-5793(90)80254-g. [DOI] [PubMed] [Google Scholar]
- Wright R. S. A reagent for the non-destructive location of steroids and some other lipophilic materials on silica gel thin-layer chromatograms. J Chromatogr. 1971 Jul 8;59(1):220–221. doi: 10.1016/s0021-9673(01)80033-9. [DOI] [PubMed] [Google Scholar]