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. 1994 Sep;176(18):5835–5842. doi: 10.1128/jb.176.18.5835-5842.1994

Sequencing analysis reveals a unique gene organization in the gyrB region of Mycoplasma hominis.

S A Ladefoged 1, G Christiansen 1
PMCID: PMC196789  PMID: 7521872

Abstract

The homolog of the gyrB gene, which has been reported to be present in the vicinity of the initiation site of replication in bacteria, was mapped on the Mycoplasma hominis genome, and the region was subsequently sequenced. Five open reading frames were identified flanking the gyrB gene, one of which showed similarity to that which encodes the LicA protein of Haemophilus influenzae. The organization of the genes in the region showed no resemblance to that in the corresponding regions of other bacteria sequenced so far. The gyrA gene was mapped 35 kb downstream from the gyrB gene.

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Selected References

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  1. Adachi T., Mizuuchi M., Robinson E. A., Appella E., O'Dea M. H., Gellert M., Mizuuchi K. DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy. Nucleic Acids Res. 1987 Jan 26;15(2):771–784. doi: 10.1093/nar/15.2.771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  3. Contreras A., Maxwell A. gyrB mutations which confer coumarin resistance also affect DNA supercoiling and ATP hydrolysis by Escherichia coli DNA gyrase. Mol Microbiol. 1992 Jun;6(12):1617–1624. doi: 10.1111/j.1365-2958.1992.tb00886.x. [DOI] [PubMed] [Google Scholar]
  4. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fujita M. Q., Yoshikawa H., Ogasawara N. Structure of the dnaA region of Pseudomonas putida: conservation among three bacteria, Bacillus subtilis, Escherichia coli and P. putida. Mol Gen Genet. 1989 Feb;215(3):381–387. doi: 10.1007/BF00427033. [DOI] [PubMed] [Google Scholar]
  6. Gellert M. DNA topoisomerases. Annu Rev Biochem. 1981;50:879–910. doi: 10.1146/annurev.bi.50.070181.004311. [DOI] [PubMed] [Google Scholar]
  7. Hansen E. B., Atlung T., Hansen F. G., Skovgaard O., von Meyenburg K. Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli. Mol Gen Genet. 1984;196(3):387–396. doi: 10.1007/BF00436184. [DOI] [PubMed] [Google Scholar]
  8. Harasawa R., Uemori T., Asada K., Kato I., Shiragami N. 'boxA'-like sequence between the 16 S/23 S spacer in rRNA operon of mycoplasmas. FEBS Lett. 1992 Feb 10;297(3):209–211. doi: 10.1016/0014-5793(92)80539-s. [DOI] [PubMed] [Google Scholar]
  9. Hattori M., Sakaki Y. Dideoxy sequencing method using denatured plasmid templates. Anal Biochem. 1986 Feb 1;152(2):232–238. doi: 10.1016/0003-2697(86)90403-3. [DOI] [PubMed] [Google Scholar]
  10. Kato J., Suzuki H., Ikeda H. Purification and characterization of DNA topoisomerase IV in Escherichia coli. J Biol Chem. 1992 Dec 25;267(36):25676–25684. [PubMed] [Google Scholar]
  11. Ladefoged S. A., Christiansen G. Analysis of the nucleotide sequence of the Mycoplasma hominis tuf gene and its flanking region. FEMS Microbiol Lett. 1991 Apr 15;63(2-3):133–139. doi: 10.1016/0378-1097(91)90075-l. [DOI] [PubMed] [Google Scholar]
  12. Ladefoged S. A., Christiansen G. Physical and genetic mapping of the genomes of five Mycoplasma hominis strains by pulsed-field gel electrophoresis. J Bacteriol. 1992 Apr;174(7):2199–2207. doi: 10.1128/jb.174.7.2199-2207.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lai C. Y., Baumann P. Genetic analysis of an aphid endosymbiont DNA fragment homologous to the rnpA-rpmH-dnaA-dnaN-gyrB region of eubacteria. Gene. 1992 Apr 15;113(2):175–181. doi: 10.1016/0378-1119(92)90393-4. [DOI] [PubMed] [Google Scholar]
  14. Margerrison E. E., Hopewell R., Fisher L. M. Nucleotide sequence of the Staphylococcus aureus gyrB-gyrA locus encoding the DNA gyrase A and B proteins. J Bacteriol. 1992 Mar;174(5):1596–1603. doi: 10.1128/jb.174.5.1596-1603.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miyata M., Sano K., Okada R., Fukumura T. Mapping of replication initiation site in Mycoplasma capricolum genome by two-dimensional gel-electrophoretic analysis. Nucleic Acids Res. 1993 Oct 11;21(20):4816–4823. doi: 10.1093/nar/21.20.4816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ogasawara N., Moriya S., von Meyenburg K., Hansen F. G., Yoshikawa H. Conservation of genes and their organization in the chromosomal replication origin region of Bacillus subtilis and Escherichia coli. EMBO J. 1985 Dec 1;4(12):3345–3350. doi: 10.1002/j.1460-2075.1985.tb04087.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ohama T., Muto A., Osawa S. Spectinomycin operon of Micrococcus luteus: evolutionary implications of organization and novel codon usage. J Mol Evol. 1989 Nov;29(5):381–395. doi: 10.1007/BF02602908. [DOI] [PubMed] [Google Scholar]
  18. Ohkubo S., Muto A., Kawauchi Y., Yamao F., Osawa S. The ribosomal protein gene cluster of Mycoplasma capricolum. Mol Gen Genet. 1987 Dec;210(2):314–322. doi: 10.1007/BF00325700. [DOI] [PubMed] [Google Scholar]
  19. Old I. G., Margarita D., Saint Girons I. Unique genetic arrangement in the dnaA region of the Borrelia burgdorferi linear chromosome: nucleotide sequence of the dnaA gene. FEMS Microbiol Lett. 1993 Jul 15;111(1):109–114. doi: 10.1111/j.1574-6968.1993.tb06369.x. [DOI] [PubMed] [Google Scholar]
  20. Parales R. E., Harwood C. S. Nucleotide sequence of the gyrB gene of Pseudomonas putida. Nucleic Acids Res. 1990 Oct 11;18(19):5880–5880. doi: 10.1093/nar/18.19.5880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Peterson S. N., Hu P. C., Bott K. F., Hutchison C. A., 3rd A survey of the Mycoplasma genitalium genome by using random sequencing. J Bacteriol. 1993 Dec;175(24):7918–7930. doi: 10.1128/jb.175.24.7918-7930.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Peterson S. N., Schramm N., Hu P. C., Bott K. F., Hutchison C. A., 3rd A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium. Nucleic Acids Res. 1991 Nov 11;19(21):6027–6031. doi: 10.1093/nar/19.21.6027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Reece R. J., Maxwell A. DNA gyrase: structure and function. Crit Rev Biochem Mol Biol. 1991;26(3-4):335–375. doi: 10.3109/10409239109114072. [DOI] [PubMed] [Google Scholar]
  24. Skovgaard O. Nucleotide sequence of a Proteus mirabilis DNA fragment homologous to the 60K-rnpA-rpmH-dnaA-dnaN-recF-gyrB region of Escherichia coli. Gene. 1990 Sep 1;93(1):27–34. doi: 10.1016/0378-1119(90)90131-a. [DOI] [PubMed] [Google Scholar]
  25. Tamura J. K., Gellert M. Characterization of the ATP binding site on Escherichia coli DNA gyrase. Affinity labeling of Lys-103 and Lys-110 of the B subunit by pyridoxal 5'-diphospho-5'-adenosine. J Biol Chem. 1990 Dec 5;265(34):21342–21349. [PubMed] [Google Scholar]
  26. Tinoco I., Jr, Uhlenbeck O. C., Levine M. D. Estimation of secondary structure in ribonucleic acids. Nature. 1971 Apr 9;230(5293):362–367. doi: 10.1038/230362a0. [DOI] [PubMed] [Google Scholar]
  27. Weiser J. N., Love J. M., Moxon E. R. The molecular mechanism of phase variation of H. influenzae lipopolysaccharide. Cell. 1989 Nov 17;59(4):657–665. doi: 10.1016/0092-8674(89)90011-1. [DOI] [PubMed] [Google Scholar]
  28. Wigley D. B., Davies G. J., Dodson E. J., Maxwell A., Dodson G. Crystal structure of an N-terminal fragment of the DNA gyrase B protein. Nature. 1991 Jun 20;351(6328):624–629. doi: 10.1038/351624a0. [DOI] [PubMed] [Google Scholar]
  29. Yamao F., Muto A., Kawauchi Y., Iwami M., Iwagami S., Azumi Y., Osawa S. UGA is read as tryptophan in Mycoplasma capricolum. Proc Natl Acad Sci U S A. 1985 Apr;82(8):2306–2309. doi: 10.1073/pnas.82.8.2306. [DOI] [PMC free article] [PubMed] [Google Scholar]

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