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. 1993 Jul;68(1):130–136. doi: 10.1038/bjc.1993.300

Expression of carcinoembryonic antigen (CEA) and nonspecific crossreacting antigen (NCA) in gastrointestinal cancer; the correlation with degree of differentiation.

Y Kodera 1, K Isobe 1, M Yamauchi 1, T Satta 1, T Hasegawa 1, S Oikawa 1, K Kondoh 1, S Akiyama 1, K Itoh 1, I Nakashima 1, et al.
PMCID: PMC1968295  PMID: 8318403

Abstract

In spite of its reputation as a tumour marker, little is known about the function of carcinoembryonic antigen (CEA). We examined the mRNA expression of CEA and NCA in 26 gastric and 14 colorectal cancers together with adjacent morphologically normal mucosae. There was no significant difference between the CEA mRNA levels of colorectal cancer and adjacent mucosa, whereas the CEA mRNA levels were significantly elevated in gastric cancer compared with normal gastric mucosa. The expression of NCA, on the other hand, was more cancer-specific and was significantly up-regulated in both gastric and colorectal cancers compared with the corresponding normal mucosae. No correlation was found between the mRNA level and plasma CEA value. No significant up-regulation was recognised in the node positive cancer, cancer with distant metastasis, or the metastatic tissues themselves. Marked diversity in the degree of differentiation in gastric cancer tissues, however, resulted in varied expression of the CEA gene family, compared with the constantly high expression found in colorectal cancer. Further analysis revealed significant up-regulation of NCA in well and moderately differentiated gastric cancers over poorly differentiated cancers, suggesting that NCA might have roles in differentiation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beauchemin N., Benchimol S., Cournoyer D., Fuks A., Stanners C. P. Isolation and characterization of full-length functional cDNA clones for human carcinoembryonic antigen. Mol Cell Biol. 1987 Sep;7(9):3221–3230. doi: 10.1128/mcb.7.9.3221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benchimol S., Fuks A., Jothy S., Beauchemin N., Shirota K., Stanners C. P. Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion molecule. Cell. 1989 Apr 21;57(2):327–334. doi: 10.1016/0092-8674(89)90970-7. [DOI] [PubMed] [Google Scholar]
  3. Chi K. F., Jessup J. M., Frazier M. L. Predominant expression of mRNA coding for nonspecific cross-reacting antigen in colorectal carcinomas. Tumour Biol. 1991;12(5):298–308. doi: 10.1159/000217718. [DOI] [PubMed] [Google Scholar]
  4. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  5. Cournoyer D., Beauchemin N., Boucher D., Benchimol S., Fuks A., Stanners C. P. Transcription of genes of the carcinoembryonic antigen family in malignant and nonmalignant human tissues. Cancer Res. 1988 Jun 1;48(11):3153–3157. [PubMed] [Google Scholar]
  6. Fantini J., Rognoni J. B., Culouscou J. M., Pommier G., Marvaldi J., Tirard A. Induction of polarized apical expression and vectorial release of carcinoembryonic antigen (CEA) during the process of differentiation of HT29-D4 cells. J Cell Physiol. 1989 Oct;141(1):126–134. doi: 10.1002/jcp.1041410119. [DOI] [PubMed] [Google Scholar]
  7. GOLD P., FREEDMAN S. O. DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES. J Exp Med. 1965 Mar 1;121:439–462. doi: 10.1084/jem.121.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hamada Y., Yamamura M., Hioki K., Yamamoto M., Nagura H., Watanabe K. Immunohistochemical study of carcinoembryonic antigen in patients with colorectal cancer. Correlation with plasma carcinoembryonic antigen levels. Cancer. 1985 Jan 1;55(1):136–141. doi: 10.1002/1097-0142(19850101)55:1<136::aid-cncr2820550121>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
  9. Hasegawa T., Isobe K., Tsuchiya Y., Oikawa S., Nakazato H., Nakashima I., Shimokata K. Nonspecific crossreacting antigen (NCA) is a major member of the carcinoembryonic antigen (CEA)-related gene family expressed in lung cancer. Br J Cancer. 1993 Jan;67(1):58–65. doi: 10.1038/bjc.1993.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hauck W., Stanners C. P. Control of carcinoembryonic antigen gene family expression in a differentiating colon carcinoma cell line, Caco-2. Cancer Res. 1991 Jul 1;51(13):3526–3533. [PubMed] [Google Scholar]
  11. Higashide T., Hinoda Y., Itoh J., Takahashi H., Satoh Y., Ibayashi Y., Imai K., Yachi A. Detection of mRNAs of carcinoembryonic antigen and nonspecific cross-reacting antigen genes in colorectal adenomas and carcinomas by in situ hybridization. Jpn J Cancer Res. 1990 Nov;81(11):1149–1154. doi: 10.1111/j.1349-7006.1990.tb02527.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hinoda Y., Takahashi H., Higashide T., Nakano T., Arimura Y., Yoshimoto M., Tsujisaki M., Imai K., Yachi A. Correlated expression of mRNAs of carcinoembryonic antigen and nonspecific cross-reacting antigen genes in malignant and nonmalignant tissues of the colon. Jpn J Clin Oncol. 1991 Apr;21(2):75–81. doi: 10.1093/oxfordjournals.jjco.a039449. [DOI] [PubMed] [Google Scholar]
  13. Hostetter R. B., Augustus L. B., Mankarious R., Chi K. F., Fan D., Toth C., Thomas P., Jessup J. M. Carcinoembryonic antigen as a selective enhancer of colorectal cancer metastasis. J Natl Cancer Inst. 1990 Mar 7;82(5):380–385. doi: 10.1093/jnci/82.5.380. [DOI] [PubMed] [Google Scholar]
  14. Kamarck M. E., Elting J. J., Hart J. T., Goebel S. J., Rae P. M., Nothdurft M. A., Nedwin J. J., Barnett T. R. Carcinoembryonic antigen family: expression in a mouse L-cell transfectant and characterization of a partial cDNA in bacteriophage lambda gt11. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5350–5354. doi: 10.1073/pnas.84.15.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kuroki M., Koga Y., Matsuoka Y. Purification and characterization of carcinoembryonic antigen-related antigens in normal adult feces. Cancer Res. 1981 Feb;41(2):713–720. [PubMed] [Google Scholar]
  16. Liotta L. A., Stetler-Stevenson W. G. Tumor invasion and metastasis: an imbalance of positive and negative regulation. Cancer Res. 1991 Sep 15;51(18 Suppl):5054s–5059s. [PubMed] [Google Scholar]
  17. Nakajima-Iijima S., Hamada H., Reddy P., Kakunaga T. Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6133–6137. doi: 10.1073/pnas.82.18.6133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Neumaier M., Zimmermann W., Shively L., Hinoda Y., Riggs A. D., Shively J. E. Characterization of a cDNA clone for the nonspecific cross-reacting antigen (NCA) and a comparison of NCA and carcinoembryonic antigen. J Biol Chem. 1988 Mar 5;263(7):3202–3207. [PubMed] [Google Scholar]
  19. Oikawa S., Nakazato H., Kosaki G. Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence. Biochem Biophys Res Commun. 1987 Jan 30;142(2):511–518. doi: 10.1016/0006-291x(87)90304-4. [DOI] [PubMed] [Google Scholar]
  20. Sato C., Miyaki M., Oikawa S., Nakazato H., Kosaki G. Differential expression of carcinoembryonic antigen and nonspecific crossreacting antigen genes in human colon adenocarcinomas and normal colon mucosa. Jpn J Cancer Res. 1988 Apr;79(4):433–437. doi: 10.1111/j.1349-7006.1988.tb01610.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schipper J. H., Frixen U. H., Behrens J., Unger A., Jahnke K., Birchmeier W. E-cadherin expression in squamous cell carcinomas of head and neck: inverse correlation with tumor dedifferentiation and lymph node metastasis. Cancer Res. 1991 Dec 1;51(23 Pt 1):6328–6337. [PubMed] [Google Scholar]
  22. Shimoyama Y., Hirohashi S. Expression of E- and P-cadherin in gastric carcinomas. Cancer Res. 1991 Apr 15;51(8):2185–2192. [PubMed] [Google Scholar]
  23. Tawaragi Y., Oikawa S., Matsuoka Y., Kosaki G., Nakazato H. Primary structure of nonspecific crossreacting antigen (NCA), a member of carcinoembryonic antigen (CEA) gene family, deduced from cDNA sequence. Biochem Biophys Res Commun. 1988 Jan 15;150(1):89–96. doi: 10.1016/0006-291x(88)90490-1. [DOI] [PubMed] [Google Scholar]
  24. Toribara N. W., Sack T. L., Gum J. R., Ho S. B., Shively J. E., Willson J. K., Kim Y. S. Heterogeneity in the induction and expression of carcinoembryonic antigen-related antigens in human colon cancer cell lines. Cancer Res. 1989 Jun 15;49(12):3321–3327. [PubMed] [Google Scholar]
  25. Zimmermann W., Ortlieb B., Friedrich R., von Kleist S. Isolation and characterization of cDNA clones encoding the human carcinoembryonic antigen reveal a highly conserved repeating structure. Proc Natl Acad Sci U S A. 1987 May;84(9):2960–2964. doi: 10.1073/pnas.84.9.2960. [DOI] [PMC free article] [PubMed] [Google Scholar]

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