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. 1993 Jul;68(1):1–7. doi: 10.1038/bjc.1993.276

The modulation of radiation-induced damage to pig skin by essential fatty acids.

J W Hopewell 1, M E Robbins 1, G J van den Aardweg 1, G M Morris 1, G A Ross 1, E Whitehouse 1, D F Horrobin 1, C A Scott 1
PMCID: PMC1968327  PMID: 8391301

Abstract

The ability of essential fatty acids (EFAs) to modulate radiation-induced normal tissue injury was assessed in pig skin. Female Large White pigs (approximately 25 Kg) received 3 ml/day orally of either an 'active' oil [So-1100, containing 9% gamma-linolenic acid (GLA)] or a 'placebo' oil (So-1129) for just 4 weeks before or for 4 weeks before and for 16 weeks after irradiation; localised irradiation of skin was with single doses of beta-rays from 22.5 mm diameter 90Sr/90Y plaques. The severity of the acute reaction, assessed in terms of erythema or moist desquamation, was significantly less in those pigs that received So-1100 both before and after irradiation, as compared with those receiving that oil only prior to irradiation and the 'placebo' groups. Dose modification factors (DMFs) of between 1.13-1.24 were obtained. A similar reduction in the severity of acute skin injury was seen in pigs receiving So-1100 for only 10 weeks after irradiation. Late skin damage, assessed in terms of late erythema or dermal necrosis, was also reduced with So-1100, with DMFs of 1.14-1.51. No such modification was observed if So-1100 was only administered for 4 weeks prior to irradiation. No adverse side-effects were apparent as a result of EFA administration. So-1100 may represent a safe and valuable method of increasing the therapeutic gain in radiotherapy.

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Selected References

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  1. Abdelaal A. S., Barker D. S., Fergusson M. M. Treatment for irradiation-induced mucositis. Lancet. 1989 Jan 14;1(8629):97–97. doi: 10.1016/s0140-6736(89)91448-7. [DOI] [PubMed] [Google Scholar]
  2. Allen J. B., Sagerman R. H., Stuart M. J. Irradiation decreases vascular prostacyclin formation with no concomitant effect on platelet thromboxane production. Lancet. 1981 Nov 28;2(8257):1193–1196. doi: 10.1016/s0140-6736(81)91437-9. [DOI] [PubMed] [Google Scholar]
  3. Blumberg A. L., Nelson D. F., Gramkowski M., Glover D., Glick J. H., Yuhas J. M., Kligerman M. M. Clinical trials of WR-2721 with radiation therapy. Int J Radiat Oncol Biol Phys. 1982 Mar-Apr;8(3-4):561–563. doi: 10.1016/0360-3016(82)90684-8. [DOI] [PubMed] [Google Scholar]
  4. Booyens J., Engelbrecht P., le Roux S., Louwrens C. C., Van der Merwe C. F., Katzeff I. E. Some effects of the essential fatty acids linoleic acid and alpha-linolenic acid and of their metabolites gamma-linolenic acid, arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid, and of prostaglandins A1 and E1 on the proliferation of human osteogenic sarcoma cells in culture. Prostaglandins Leukot Med. 1984 Jul;15(1):15–33. doi: 10.1016/0262-1746(84)90053-2. [DOI] [PubMed] [Google Scholar]
  5. Bégin M. E., Ells G., Das U. N., Horrobin D. F. Differential killing of human carcinoma cells supplemented with n-3 and n-6 polyunsaturated fatty acids. J Natl Cancer Inst. 1986 Nov;77(5):1053–1062. [PubMed] [Google Scholar]
  6. Bégin M. E., Ells G., Das U. N. Selected fatty acids as possible intermediates for selective cytotoxic activity of anticancer agents involving oxygen radicals. Anticancer Res. 1986 Mar-Apr;6(2):291–295. [PubMed] [Google Scholar]
  7. Dion M. W., Hussey D. H., Doornbos J. F., Vigliotti A. P., Wen B. C., Anderson B. Preliminary results of a pilot study of pentoxifylline in the treatment of late radiation soft tissue necrosis. Int J Radiat Oncol Biol Phys. 1990 Aug;19(2):401–407. doi: 10.1016/0360-3016(90)90549-y. [DOI] [PubMed] [Google Scholar]
  8. Dion M. W., Hussey D. H., Osborne J. W. The effect of pentoxifylline on early and late radiation injury following fractionated irradiation in C3H mice. Int J Radiat Oncol Biol Phys. 1989 Jul;17(1):101–107. doi: 10.1016/0360-3016(89)90376-3. [DOI] [PubMed] [Google Scholar]
  9. Eldor A., Vlodavsky I., Fuks Z., Matzner Y., Rubin D. B. Arachidonic metabolism and radiation toxicity in cultures of vascular endothelial cells. Prostaglandins Leukot Essent Fatty Acids. 1989 Jun;36(4):251–258. doi: 10.1016/0952-3278(89)90137-3. [DOI] [PubMed] [Google Scholar]
  10. Hanson W. R., Ainsworth E. J. 16,16-Dimethyl prostaglandin E2 induces radioprotection in murine intestinal and hematopoietic stem cells. Radiat Res. 1985 Aug;103(2):196–203. [PubMed] [Google Scholar]
  11. Hopewell J. W. Mechanisms of the action of radiation on skin and underlying tissues. Br J Radiol Suppl. 1986;19:39–47. [PubMed] [Google Scholar]
  12. Hopewell J. W., van den Aardweg G. J. Radiobiological studies with pig skin. Int J Radiat Oncol Biol Phys. 1988 May;14(5):1047–1050. doi: 10.1016/0360-3016(88)90031-4. [DOI] [PubMed] [Google Scholar]
  13. Hornsey S., Myers R., Jenkinson T. The reduction of radiation damage to the spinal cord by post-irradiation administration of vasoactive drugs. Int J Radiat Oncol Biol Phys. 1990 Jun;18(6):1437–1442. doi: 10.1016/0360-3016(90)90319-f. [DOI] [PubMed] [Google Scholar]
  14. Horrobin D. F., Ells K. M., Morse-Fisher N., Manku M. S. The effects of evening primrose oil, safflower oil and paraffin on plasma fatty acid levels in humans: choice of an appropriate placebo for clinical studies on primrose oil. Prostaglandins Leukot Essent Fatty Acids. 1991 Apr;42(4):245–249. doi: 10.1016/0952-3278(91)90090-r. [DOI] [PubMed] [Google Scholar]
  15. Horrobin D. F. Is the main problem in free radical damage caused by radiation, oxygen and other toxins the loss of membrane essential fatty acids rather than the accumulation of toxic materials? Med Hypotheses. 1991 May;35(1):23–26. doi: 10.1016/0306-9877(91)90078-d. [DOI] [PubMed] [Google Scholar]
  16. Horrobin D. F. Nutritional and medical importance of gamma-linolenic acid. Prog Lipid Res. 1992;31(2):163–194. doi: 10.1016/0163-7827(92)90008-7. [DOI] [PubMed] [Google Scholar]
  17. Levi S., Goodlad R. A., Lee C. Y., Stamp G., Walport M. J., Wright N. A., Hodgson H. J. Inhibitory effect of non-steroidal anti-inflammatory drugs on mucosal cell proliferation associated with gastric ulcer healing. Lancet. 1990 Oct 6;336(8719):840–843. doi: 10.1016/0140-6736(90)92341-e. [DOI] [PubMed] [Google Scholar]
  18. Overgaard J., Hansen H. S., Andersen A. P., Hjelm-Hansen M., Jørgensen K., Sandberg E., Berthelsen A., Hammer R., Pedersen M. Misonidazole combined with split-course radiotherapy in the treatment of invasive carcinoma of larynx and pharynx: report from the DAHANCA 2 study. Int J Radiat Oncol Biol Phys. 1989 Apr;16(4):1065–1068. doi: 10.1016/0360-3016(89)90917-6. [DOI] [PubMed] [Google Scholar]
  19. Robbins M. E., Hopewell J. W. Physiological factors effecting renal radiation tolerance: a guide to the treatment of late effects. Br J Cancer Suppl. 1986;7:265–267. [PMC free article] [PubMed] [Google Scholar]
  20. Rubin D. B., Drab E. A., Ward W. F. Physiological and biochemical markers of the endothelial cell response to irradiation. Int J Radiat Biol. 1991 Jul-Aug;60(1-2):29–32. doi: 10.1080/09553009114551461. [DOI] [PubMed] [Google Scholar]
  21. Schneidkraut M. J., Kot P. A., Ramwell P. W., Rose J. C. Thromboxane and prostacyclin synthesis following whole body irradiation in rats. J Appl Physiol Respir Environ Exerc Physiol. 1984 Sep;57(3):833–838. doi: 10.1152/jappl.1984.57.3.833. [DOI] [PubMed] [Google Scholar]
  22. Sinzinger H., Cromwell M., Firbas W. Long-lasting depression of rabbit aortic prostacyclin formation by single-dose irradiation. Radiat Res. 1984 Mar;97(3):533–536. [PubMed] [Google Scholar]
  23. Stark G. The effect of ionizing radiation on lipid membranes. Biochim Biophys Acta. 1991 Jul 22;1071(2):103–122. doi: 10.1016/0304-4157(91)90020-w. [DOI] [PubMed] [Google Scholar]
  24. Tochner Z., Barnes M., Mitchell J. B., Orr K., Glatstein E., Russo A. Protection by indomethacin against acute radiation esophagitis. Digestion. 1990;47(2):81–87. doi: 10.1159/000200480. [DOI] [PubMed] [Google Scholar]
  25. Ward W. F., Molteni A., Ts'ao C. H., Kim Y. T., Hinz J. M. Radiation pneumotoxicity in rats: modification by inhibitors of angiotensin converting enzyme. Int J Radiat Oncol Biol Phys. 1992;22(3):623–625. doi: 10.1016/0360-3016(92)90890-t. [DOI] [PubMed] [Google Scholar]
  26. Ward W. F., Molteni A., Ts'ao C., Hinz J. M. The effect of Captopril on benign and malignant reactions in irradiated rat skin. Br J Radiol. 1990 May;63(749):349–354. doi: 10.1259/0007-1285-63-749-349. [DOI] [PubMed] [Google Scholar]
  27. Willis A. L. Nutritional and pharmacological factors in eicosanoid biology. Nutr Rev. 1981 Aug;39(8):289–301. doi: 10.1111/j.1753-4887.1981.tb06794.x. [DOI] [PubMed] [Google Scholar]
  28. Zurier R. B. Prostaglandin E1: is it useful? J Rheumatol. 1990 Nov;17(11):1439–1441. [PubMed] [Google Scholar]
  29. van den Aardweg G. J., Hopewell J. W., Simmonds R. H. Repair and recovery in the epithelial and vascular connective tissues of pig skin after irradiation. Radiother Oncol. 1988 Jan;11(1):73–82. doi: 10.1016/0167-8140(88)90047-3. [DOI] [PubMed] [Google Scholar]

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