Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1993 Sep;68(3):460–464. doi: 10.1038/bjc.1993.370

Involvement of RET oncogene in human tumours: specificity of RET activation to thyroid tumours.

M Santoro 1, N Sabino 1, Y Ishizaka 1, T Ushijima 1, F Carlomagno 1, A Cerrato 1, M Grieco 1, C Battaglia 1, M L Martelli 1, C Paulin 1, et al.
PMCID: PMC1968385  PMID: 8353035

Abstract

Non-thyroid neoplasia were analysed by Southern blot of genomic DNA and DNA prepared by reverse transcription and amplification by polymerase chain reaction (RT/PCR) for the activation of the RET oncogene. It is known that the rearrangement of RET occurs in about 10%-20% of human thyroid papillary carcinomas. None of 528 non-thyroid tumours showed rearrangement of the RET proto-oncogene, whereas three out of 30 thyroid papillary carcinomas were positive for RET activation. Therefore the activation of RET seems to be a somatic cell mutation specific to human thyroid carcinomas.

Full text

PDF
460

Images in this article

462Figure 2
on p.462
463Figure 3
on p.463

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambesi-Impiombato F. S., Parks L. A., Coon H. G. Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3455–3459. doi: 10.1073/pnas.77.6.3455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bongarzone I., Monzini N., Borrello M. G., Carcano C., Ferraresi G., Arighi E., Mondellini P., Della Porta G., Pierotti M. A. Molecular characterization of a thyroid tumor-specific transforming sequence formed by the fusion of ret tyrosine kinase and the regulatory subunit RI alpha of cyclic AMP-dependent protein kinase A. Mol Cell Biol. 1993 Jan;13(1):358–366. doi: 10.1128/mcb.13.1.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bongarzone I., Pierotti M. A., Monzini N., Mondellini P., Manenti G., Donghi R., Pilotti S., Grieco M., Santoro M., Fusco A. High frequency of activation of tyrosine kinase oncogenes in human papillary thyroid carcinoma. Oncogene. 1989 Dec;4(12):1457–1462. [PubMed] [Google Scholar]
  4. Bonner T. I., Oppermann H., Seeburg P., Kerby S. B., Gunnell M. A., Young A. C., Rapp U. R. The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 gene. Nucleic Acids Res. 1986 Jan 24;14(2):1009–1015. doi: 10.1093/nar/14.2.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  6. Fusco A., Berlingieri M. T., Di Fiore P. P., Portella G., Grieco M., Vecchio G. One- and two-step transformations of rat thyroid epithelial cells by retroviral oncogenes. Mol Cell Biol. 1987 Sep;7(9):3365–3370. doi: 10.1128/mcb.7.9.3365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grieco M., Santoro M., Berlingieri M. T., Melillo R. M., Donghi R., Bongarzone I., Pierotti M. A., Della Porta G., Fusco A., Vecchio G. PTC is a novel rearranged form of the ret proto-oncogene and is frequently detected in vivo in human thyroid papillary carcinomas. Cell. 1990 Feb 23;60(4):557–563. doi: 10.1016/0092-8674(90)90659-3. [DOI] [PubMed] [Google Scholar]
  8. Ishizaka Y., Ochiai M., Tahira T., Sugimura T., Nagao M. Activation of the ret-II oncogene without a sequence encoding a transmembrane domain and transforming activity of two ret-II oncogene products differing in carboxy-termini due to alternative splicing. Oncogene. 1989 Jun;4(6):789–794. [PubMed] [Google Scholar]
  9. Ishizaka Y., Shima H., Sugimura T., Nagao M. Detection of phosphorylated retTPC oncogene product in cytoplasm. Oncogene. 1992 Jul;7(7):1441–1444. [PubMed] [Google Scholar]
  10. Ishizaka Y., Ushijima T., Sugimura T., Nagao M. cDNA cloning and characterization of ret activated in a human papillary thyroid carcinoma cell line. Biochem Biophys Res Commun. 1990 Apr 30;168(2):402–408. doi: 10.1016/0006-291x(90)92335-w. [DOI] [PubMed] [Google Scholar]
  11. Iwamoto T., Takahashi M., Ito M., Hamaguchi M., Isobe K., Misawa N., Asai J., Yoshida T., Nakashima I. Oncogenicity of the ret transforming gene in MMTV/ret transgenic mice. Oncogene. 1990 Apr;5(4):535–542. [PubMed] [Google Scholar]
  12. Iwamoto T., Takahashi M., Ito M., Hamatani K., Ohbayashi M., Wajjwalku W., Isobe K., Nakashima I. Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene. EMBO J. 1991 Nov;10(11):3167–3175. doi: 10.1002/j.1460-2075.1991.tb04878.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jhiang S. M., Caruso D. R., Gilmore E., Ishizaka Y., Tahira T., Nagao M., Chiu I. M., Mazzaferri E. L. Detection of the PTC/retTPC oncogene in human thyroid cancers. Oncogene. 1992 Jul;7(7):1331–1337. [PubMed] [Google Scholar]
  14. Koda T. [ret gene from a human stomach cancer]. Hokkaido Igaku Zasshi. 1988 Nov;63(6):913–924. [PubMed] [Google Scholar]
  15. Lanzi C., Borrello M. G., Bongarzone I., Migliazza A., Fusco A., Grieco M., Santoro M., Gambetta R. A., Zunino F., Della Porta G. Identification of the product of two oncogenic rearranged forms of the RET proto-oncogene in papillary thyroid carcinomas. Oncogene. 1992 Nov;7(11):2189–2194. [PubMed] [Google Scholar]
  16. Pierotti M. A., Santoro M., Jenkins R. B., Sozzi G., Bongarzone I., Grieco M., Monzini N., Miozzo M., Herrmann M. A., Fusco A. Characterization of an inversion on the long arm of chromosome 10 juxtaposing D10S170 and RET and creating the oncogenic sequence RET/PTC. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1616–1620. doi: 10.1073/pnas.89.5.1616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Santoro M., Carlomagno F., Hay I. D., Herrmann M. A., Grieco M., Melillo R., Pierotti M. A., Bongarzone I., Della Porta G., Berger N. Ret oncogene activation in human thyroid neoplasms is restricted to the papillary cancer subtype. J Clin Invest. 1992 May;89(5):1517–1522. doi: 10.1172/JCI115743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tahira T., Ishizaka Y., Itoh F., Sugimura T., Nagao M. Characterization of ret proto-oncogene mRNAs encoding two isoforms of the protein product in a human neuroblastoma cell line. Oncogene. 1990 Jan;5(1):97–102. [PubMed] [Google Scholar]
  19. Takahashi M., Buma Y., Iwamoto T., Inaguma Y., Ikeda H., Hiai H. Cloning and expression of the ret proto-oncogene encoding a tyrosine kinase with two potential transmembrane domains. Oncogene. 1988 Nov;3(5):571–578. [PubMed] [Google Scholar]
  20. Takahashi M., Buma Y., Taniguchi M. Identification of the ret proto-oncogene products in neuroblastoma and leukemia cells. Oncogene. 1991 Feb;6(2):297–301. [PubMed] [Google Scholar]
  21. Takahashi M., Ritz J., Cooper G. M. Activation of a novel human transforming gene, ret, by DNA rearrangement. Cell. 1985 Sep;42(2):581–588. doi: 10.1016/0092-8674(85)90115-1. [DOI] [PubMed] [Google Scholar]
  22. Wajjwalku W., Nakamura S., Hasegawa Y., Miyazaki K., Satoh Y., Funahashi H., Matsuyama M., Takahashi M. Low frequency of rearrangements of the ret and trk proto-oncogenes in Japanese thyroid papillary carcinomas. Jpn J Cancer Res. 1992 Jul;83(7):671–675. doi: 10.1111/j.1349-7006.1992.tb01963.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES