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. 1993 Sep;68(3):515–518. doi: 10.1038/bjc.1993.378

Enhancement by thyroxine of gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine in Wistar rats.

H Iishi 1, M Tatsuta 1, M Baba 1, R Yamamoto 1, H Taniguchi 1
PMCID: PMC1968395  PMID: 8353042

Abstract

The affects of L-thyroxine (T4) on the incidence and histology of gastric cancers induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and on the labelling index of gastric mucosal epithelial cells were investigated in Wistar rats. After oral treatment with MNNG for 25 weeks, the rats received s.c. injections of T4 (0.2 microgram kg-1) in depot form every other day until the end of the experiment in Week 52. This long-term treatment with T4 significantly increased the incidence of gastric cancers in Week 52. However, it did not influence the histological type of the gastric cancers. It also caused significant increases in the labelling indices of the fundic and antral epithelial cells. These findings indicate that T4 enhances the development of gastric cancers, and that its effect may be related to its effect in increasing proliferation of gastric epithelial cells.

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Selected References

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  1. Adeniyi K. O., Olowookorun M. O. Gastric acid secretion and parietal cell mass: effects of thyroidectomy and thyroxine. Am J Physiol. 1989 Jun;256(6 Pt 1):G975–G978. doi: 10.1152/ajpgi.1989.256.6.G975. [DOI] [PubMed] [Google Scholar]
  2. Bachman S. E., Mashaly M. M. Relationship between circulating thyroid hormones and humoral immunity in immature male chickens. Dev Comp Immunol. 1986 Summer;10(3):395–403. doi: 10.1016/0145-305x(86)90029-7. [DOI] [PubMed] [Google Scholar]
  3. Berelowitz M., Maeda K., Harris S., Frohman L. A. The effect of alterations in the pituitary-thyroid axis on hypothalamic content and in vitro release of somatostatin-like immunoreactivity. Endocrinology. 1980 Jul;107(1):24–29. doi: 10.1210/endo-107-1-24. [DOI] [PubMed] [Google Scholar]
  4. Cohen S. The epidermal growth factor (EGF). Cancer. 1983 May 15;51(10):1787–1791. doi: 10.1002/1097-0142(19830515)51:10<1787::aid-cncr2820511004>3.0.co;2-a. [DOI] [PubMed] [Google Scholar]
  5. Eastwood G. L., Quimby G. F. Effect of chronic cimetidine ingestion on fundic and antral epithelial proliferation in the rat. Dig Dis Sci. 1983 Jan;28(1):61–64. doi: 10.1007/BF01393362. [DOI] [PubMed] [Google Scholar]
  6. Gratzner H. G. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science. 1982 Oct 29;218(4571):474–475. doi: 10.1126/science.7123245. [DOI] [PubMed] [Google Scholar]
  7. Gupta M. K., Chiang T., Deodhar S. D. Effect of thyroxine on immune response in C57Bl/6J mice. Acta Endocrinol (Copenh) 1983 May;103(1):76–80. doi: 10.1530/acta.0.1030076. [DOI] [PubMed] [Google Scholar]
  8. Hernandez D. E., Walker C. H., Mason G. A. Influence of thyroid states on stress gastric ulcer formation. Life Sci. 1988;42(18):1757–1764. doi: 10.1016/0024-3205(88)90042-2. [DOI] [PubMed] [Google Scholar]
  9. Hochman P. S., Cudkowicz G. Suppression of natural cytotoxicity by spleen cells of hydrocortisone-treated mice. J Immunol. 1979 Sep;123(3):968–976. [PubMed] [Google Scholar]
  10. Iishi H., Tatsuta M., Baba M., Okuda S., Taniguchi H. Enhancement by thyroxine of experimental carcinogenesis induced in rat colon by azoxymethane. Int J Cancer. 1992 Apr 1;50(6):974–976. doi: 10.1002/ijc.2910500625. [DOI] [PubMed] [Google Scholar]
  11. Kobori O., Watanabe J., Shimizu T., Shoji M., Morioka Y. Enhancing effect of sodium taurocholate on N-methyl-N'-nitro-N-nitrosoguanidine-induced stomach tumorigenesis in rats. Gan. 1984 Aug;75(8):651–654. [PubMed] [Google Scholar]
  12. Milisauskas V. K., Cudkowicz G., Nakamura I. Role of suppressor cells in the decline of natural killer cell activity in estrogen-treated mice. Cancer Res. 1983 Nov;43(11):5240–5243. [PubMed] [Google Scholar]
  13. Morstyn G., Hsu S. M., Kinsella T., Gratzner H., Russo A., Mitchell J. B. Bromodeoxyuridine in tumors and chromosomes detected with a monoclonal antibody. J Clin Invest. 1983 Nov;72(5):1844–1850. doi: 10.1172/JCI111145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oppenheimer J. H. Thyroid hormone action at the cellular level. Science. 1979 Mar 9;203(4384):971–979. doi: 10.1126/science.218285. [DOI] [PubMed] [Google Scholar]
  15. Pierpaoli W., Baroni C., Fabris N., Sorkin E. Hormones and immunological capacity. II. Reconstitution of antibody production in hormonally deficient mice by somatotropic hormone, thyrotropic hormone and thyroxin. Immunology. 1969 Feb;16(2):217–230. [PMC free article] [PubMed] [Google Scholar]
  16. Stefanini M., De Martino C., Zamboni L. Fixation of ejaculated spermatozoa for electron microscopy. Nature. 1967 Oct 14;216(5111):173–174. doi: 10.1038/216173a0. [DOI] [PubMed] [Google Scholar]
  17. Takahashi M., Hasegawa R., Furukawa F., Toyoda K., Sato H., Hayashi Y. Effects of ethanol, potassium metabisulfite, formaldehyde and hydrogen peroxide on gastric carcinogenesis in rats after initiation with N-methyl-N'-nitro-N-nitrosoguanidine. Jpn J Cancer Res. 1986 Feb;77(2):118–124. [PubMed] [Google Scholar]
  18. Tatsuta M., Iishi H., Baba M., Taniguchi H. Enhancement by somatostatin of experimental gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine in Wistar rats. Cancer Res. 1989 Oct 15;49(20):5534–5536. [PubMed] [Google Scholar]
  19. Tatsuta M., Iishi H., Baba M., Yamamura H., Taniguchi H. Enhancement by prolonged administration of caerulein of experimental carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine in rat stomach. Cancer Res. 1988 Nov 15;48(22):6332–6335. [PubMed] [Google Scholar]
  20. Tatsuta M., Iishi H., Yamamura H., Baba M., Yamamoto R., Taniguchi H. Effect of cimetidine on inhibition by tetragastrin of carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine in Wistar rats. Cancer Res. 1988 Mar 15;48(6):1591–1595. [PubMed] [Google Scholar]
  21. Walker P., Weichsel M. E., Jr, Hoath S. B., Poland R. E., Fisher D. A. Effect of thyroxine, testosterone, and corticosterone on nerve growth factor (NGF) and epidermal growth factor (EGF) concentrations in adult female mouse submaxillary gland: dissociation of NGF and EGF responses. Endocrinology. 1981 Aug;109(2):582–587. doi: 10.1210/endo-109-2-582. [DOI] [PubMed] [Google Scholar]
  22. Watanabe H., Takahashi T., Okamoto T., Ogundigie P. O., Ito A. Effects of sodium chloride and ethanol on stomach tumorigenesis in ACI rats treated with N-methyl-N'-nitro-N-nitrosoguanidine: a quantitative morphometric approach. Jpn J Cancer Res. 1992 Jun;83(6):588–593. doi: 10.1111/j.1349-7006.1992.tb00130.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yasui W., Takekura N., Kameda T., Oda N., Ito M., Ito H., Tahara E. Effect of epidermal growth factor on rat stomach carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine. Acta Pathol Jpn. 1990 Mar;40(3):165–171. doi: 10.1111/j.1440-1827.1990.tb03318.x. [DOI] [PubMed] [Google Scholar]
  24. de Launoit Y., Kiss R. Influence of L-thyroxine, L-triiodothyronine, thyroid stimulating hormone, or estradiol on the cell kinetics of cultured mammary cancer cells. In Vitro Cell Dev Biol. 1989 Jul;25(7):585–591. doi: 10.1007/BF02623627. [DOI] [PubMed] [Google Scholar]

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