Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Oct;176(20):6407–6409. doi: 10.1128/jb.176.20.6407-6409.1994

The presence of an active S-adenosylmethionine decarboxylase gene increases the growth defect observed in Saccharomyces cerevisiae mutants unable to synthesize putrescine, spermidine, and spermine.

D Balasundaram 1, Q W Xie 1, C W Tabor 1, H Tabor 1
PMCID: PMC196985  PMID: 7929015

Abstract

Saccharomyces cerevisiae spe1 delta SPE2 mutants (lacking ornithine decarboxylase) and spe1 delta spe2 delta mutants (lacking both ornithine decarboxylase and S-adenosylmethionine decarboxylase) are equally unable to synthesize putrescine, spermidine, and spermine and require spermidine or spermine for growth in amine-free media. The cessation of growth, however, occurs more rapidly in spe1 delta SPE2 cells than in SPE1 spe2 delta or spe1 delta spe2 delta cells. Since spe1 delta SPE2 cells can synthesize decarboxylated adenosylmethionine (dcAdoMet), these data indicate that dcAdoMet may be toxic to amine-deficient cells.

Full text

PDF
6408

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bacchi C. J., Garofalo J., Ciminelli M., Rattendi D., Goldberg B., McCann P. P., Yarlett N. Resistance to DL-alpha-difluoromethylornithine by clinical isolates of Trypanosoma brucei rhodesiense. Role of S-adenosylmethionine. Biochem Pharmacol. 1993 Aug 3;46(3):471–481. doi: 10.1016/0006-2952(93)90524-z. [DOI] [PubMed] [Google Scholar]
  2. Balasundaram D., Tabor C. W., Tabor H. Oxygen toxicity in a polyamine-depleted spe2 delta mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1993 May 15;90(10):4693–4697. doi: 10.1073/pnas.90.10.4693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Balasundaram D., Tabor C. W., Tabor H. Spermidine or spermine is essential for the aerobic growth of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5872–5876. doi: 10.1073/pnas.88.13.5872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohn M. S., Tabor C. W., Tabor H. Isolation and characterization of Saccharomyces cerevisiae mutants deficient in S-adenosylmethionine decarboxylase, spermidine, and spermine. J Bacteriol. 1978 Apr;134(1):208–213. doi: 10.1128/jb.134.1.208-213.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohn M. S., Tabor C. W., Tabor H. Regulatory mutations affecting ornithine decarboxylase activity in Saccharomyces cerevisiae. J Bacteriol. 1980 Jun;142(3):791–799. doi: 10.1128/jb.142.3.791-799.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fonzi W. A. Biochemical and genetic characterization of the structure of yeast ornithine decarboxylase. Biochem Biophys Res Commun. 1989 Aug 15;162(3):1409–1416. doi: 10.1016/0006-291x(89)90831-0. [DOI] [PubMed] [Google Scholar]
  7. Fonzi W. A., Sypherd P. S. Expression of the gene for ornithine decarboxylase of Saccharomyces cerevisiae in Escherichia coli. Mol Cell Biol. 1985 Jan;5(1):161–166. doi: 10.1128/mcb.5.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hill J. E., Myers A. M., Koerner T. J., Tzagoloff A. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast. 1986 Sep;2(3):163–167. doi: 10.1002/yea.320020304. [DOI] [PubMed] [Google Scholar]
  9. Ito H., Fukuda Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. doi: 10.1128/jb.153.1.163-168.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mamont P. S., Danzin C., Wagner J., Siat M., Joder-Ohlenbusch A. M., Claverie N. Accumulation of decarboxylated S-adenosyl-L-methionine in mammalian cells as a consequence of the inhibition of putrescine biosynthesis. Eur J Biochem. 1982 Apr;123(3):499–504. doi: 10.1111/j.1432-1033.1982.tb06559.x. [DOI] [PubMed] [Google Scholar]
  11. Marton L. J., Lee P. L. More sensitive automated detection of polyamines in physiological fluids and tissue extracts with omicron-phthalaldehyde. Clin Chem. 1975 Nov;21(12):1721–1724. [PubMed] [Google Scholar]
  12. Pegg A. E., Pösö H., Shuttleworth K., Bennett R. A. Effect of inhibition of polyamine synthesis on the content of decarboxylated S-adenosylmethionine. Biochem J. 1982 Feb 15;202(2):519–526. doi: 10.1042/bj2020519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pegg A. E. The role of polyamine depletion and accumulation of decarboxylated S-adenosylmethionine in the inhibition of growth of SV-3T3 cells treated with alpha-difluoromethylornithine. Biochem J. 1984 Nov 15;224(1):29–38. doi: 10.1042/bj2240029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pegg A. E., Wechter R. S., Clark R. S., Wiest L., Erwin B. G. Acetylation of decarboxylated S-adenosylmethionine by mammalian cells. Biochemistry. 1986 Jan 28;25(2):379–384. doi: 10.1021/bi00350a016. [DOI] [PubMed] [Google Scholar]
  15. Rothstein R. J. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. doi: 10.1016/0076-6879(83)01015-0. [DOI] [PubMed] [Google Scholar]
  16. Wagner J., Danzin C., Mamont P. Reversed-phase ion-pair liquid chromatographic procedure for the simultaneous analysis of S-adenosylmethionine, its metabolites and the natural polyamines. J Chromatogr. 1982 Feb 12;227(2):349–368. doi: 10.1016/s0378-4347(00)80389-8. [DOI] [PubMed] [Google Scholar]
  17. Xie Q. W., Tabor C. W., Tabor H. Ornithine decarboxylase in Saccharomyces cerevisiae: chromosomal assignment and genetic mapping of the SPE1 gene. Yeast. 1990 Nov-Dec;6(6):455–460. doi: 10.1002/yea.320060602. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES