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. 1990 Jan;61(1):96–100. doi: 10.1038/bjc.1990.21

Investigation of expression of 5T4 antigen in cervical cancer.

H Jones 1, G Roberts 1, N Hole 1, I W McDicken 1, P Stern 1
PMCID: PMC1971310  PMID: 2404512

Abstract

A monoclonal antibody detecting amniotrophoblastic antigen 5T4 has shown reactivity against various neoplastic cell lines and tumour specimens but with a relatively restricted normal tissue expression. This antibody has been investigated as a potential indicator of premalignant changes identified as cervical intra-epithelial neoplasia and malignant cervical lesions using immunohistochemistry on frozen tissue biopsies. The basal cells of normal cervical stratified epithelium exhibited faint staining, but a general increase in intensity and extent of specific labeling of this tissue was seen from the first premalignant stage through to carcinoma. In most cases, this was in accordance with the distribution of dysplastic cells, and was accompanied by increased specific staining of the stromal tissue. All invasive squamous carcinomas of the cervix were 5T4 antigen positive. Common inflammatory non-malignant diseases did show a certain degree of epithelial and stromal reactivity. These results, showing 5T4 reactivity with neoplastic and pre-neoplastic lesions, may provide a quantitative basis for its potential use as a tumour marker in the immunochemical detection on immunoassay of cervical cancer.

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Selected References

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  1. Anderson M. C. The pathology of cervical cancer. Clin Obstet Gynaecol. 1985 Mar;12(1):87–119. [PubMed] [Google Scholar]
  2. Brescia R. J., Jenson A. B., Lancaster W. D., Kurman R. J. The role of human papillomaviruses in the pathogenesis and histologic classification of precancerous lesions of the cervix. Hum Pathol. 1986 Jun;17(6):552–559. doi: 10.1016/s0046-8177(86)80126-5. [DOI] [PubMed] [Google Scholar]
  3. Buckley C. H., Beards C. S., Fox H. Pathological prognostic indicators in cervical cancer with particular reference to patients under the age of 40 years. Br J Obstet Gynaecol. 1988 Jan;95(1):47–56. doi: 10.1111/j.1471-0528.1988.tb06479.x. [DOI] [PubMed] [Google Scholar]
  4. Buckley C. H., Butler E. B., Fox H. Cervical intraepithelial neoplasia. J Clin Pathol. 1982 Jan;35(1):1–13. doi: 10.1136/jcp.35.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bulmer J. N., Sunderland C. A. Bone-marrow origin of endometrial granulocytes in the early human placental bed. J Reprod Immunol. 1983 Nov;5(6):383–387. doi: 10.1016/0165-0378(83)90247-4. [DOI] [PubMed] [Google Scholar]
  6. Epenetos A. A., Snook D., Hooker G., Begent R., Durbin H., Oliver R. T., Bodmer W. F., Lavender J. P. Indium-111 labelled monoclonal antibody to placental alkaline phosphatase in in the detection of neoplasms of testis, ovary, and cervix. Lancet. 1985 Aug 17;2(8451):350–353. doi: 10.1016/s0140-6736(85)92495-x. [DOI] [PubMed] [Google Scholar]
  7. GALVIN G. A., JONES H. W., TE LINDE R. W. The significance of basal-cell hyperactivity in cervical biopsies. Am J Obstet Gynecol. 1955 Oct;70(4):808–821. doi: 10.1016/s0002-9378(16)37837-1. [DOI] [PubMed] [Google Scholar]
  8. Hare M. J., Taylor-Robinson D., Cooper P. Evidence for an association between Chlamydia trachomatis and cervical intraepithelial neoplasia. Br J Obstet Gynaecol. 1982 Jun;89(6):489–492. doi: 10.1111/j.1471-0528.1982.tb03643.x. [DOI] [PubMed] [Google Scholar]
  9. Hole N., Stern P. L. A 72 kD trophoblast glycoprotein defined by a monoclonal antibody. Br J Cancer. 1988 Mar;57(3):239–246. doi: 10.1038/bjc.1988.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jha R. S., Wickenden C., Anderson M. C., Coleman D. V. Monoclonal antibodies for the histopathological diagnosis of cervical neoplasia. Br J Obstet Gynaecol. 1984 May;91(5):483–488. doi: 10.1111/j.1471-0528.1984.tb04788.x. [DOI] [PubMed] [Google Scholar]
  11. Lindgren J., Vesterinen E., Purola E., Wahlstrom T. Prognostic significance of tissue carcinoembryonic antigen in mild dysplasia of the uterine cervix. Tumour Biol. 1986;6(5):465–470. [PubMed] [Google Scholar]
  12. McCance D. J., Campion M. J., Clarkson P. K., Chesters P. M., Jenkins D., Singer A. Prevalence of human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and invasive carcinoma of the cervix. Br J Obstet Gynaecol. 1985 Nov;92(11):1101–1105. doi: 10.1111/j.1471-0528.1985.tb03019.x. [DOI] [PubMed] [Google Scholar]
  13. McDicken I. W., McLaughlin P. J., Tromans P. M., Luesley D. M., Johnson P. M. Detection of placental-type alkaline phosphatase in ovarian cancer. Br J Cancer. 1985 Jul;52(1):59–64. doi: 10.1038/bjc.1985.149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McDicken I. W., Stamp G. H., McLaughlin P. J., Johnson P. M. Expression of human placental-type alkaline phosphatase in primary breast cancer. Int J Cancer. 1983 Aug 15;32(2):205–209. doi: 10.1002/ijc.2910320211. [DOI] [PubMed] [Google Scholar]
  15. McLaughlin P. J., Warne P. H., Hutchinson G. E., Johnson P. M., Tucker D. F. Placental-type alkaline phosphatase in cervical neoplasia. Br J Cancer. 1987 Feb;55(2):197–201. doi: 10.1038/bjc.1987.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. STERN E., NEELY P. M. DYSPLASIA OF THE UTERINE CERVIX. INCIDENCE OF REGRESSION, RECURRENCE, AND CANCER. Cancer. 1964 Apr;17:508–512. doi: 10.1002/1097-0142(196404)17:4<508::aid-cncr2820170412>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  17. Sikora K., Smedley H., Thorpe P. Tumour imaging and drug targeting. Br Med Bull. 1984 Jul;40(3):233–239. doi: 10.1093/oxfordjournals.bmb.a071983. [DOI] [PubMed] [Google Scholar]
  18. Smith N. C., Brush M. G., Luckett S. Preparation of human placental villous surface membrane. Nature. 1974 Nov 22;252(5481):302–303. doi: 10.1038/252302b0. [DOI] [PubMed] [Google Scholar]
  19. Southall P. J., Boxer G. M., Bagshawe K. D., Hole N., Bromley M., Stern P. L. Immunohistological distribution of 5T4 antigen in normal and malignant tissues. Br J Cancer. 1990 Jan;61(1):89–95. doi: 10.1038/bjc.1990.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Travers P., Bodmer W. Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants. Int J Cancer. 1984 May 15;33(5):633–641. doi: 10.1002/ijc.2910330514. [DOI] [PubMed] [Google Scholar]
  21. Villard L., Murphy M., Vessey M. P. Cervical cancer deaths in young women. Lancet. 1989 Feb 18;1(8634):377–377. doi: 10.1016/s0140-6736(89)91743-1. [DOI] [PubMed] [Google Scholar]

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