Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1984 Apr;49(4):485–494. doi: 10.1038/bjc.1984.76

Lack of correlation between lymphocyte activating determinants and HLA-DR on acute leukaemias.

G M Taylor, J C Ridway, W D Fergusson, R Harris
PMCID: PMC1976761  PMID: 6201182

Abstract

The expression of allogenic lymphocyte-activating determinants (LAD) on 25 acute leukaemias has been compared with the expression of cell-surface antigens identified by HLA-DR allo- and xeno-antisera. The close correlation between LAD and DR known to occur on normal lymphocytes was not found in leukaemias. Twenty-two LAD+ leukaemias included 2 DR- cases, whilst 2 LAD- leukaemias were DR+. With the exception of 3 leukaemias all were strongly beta 2 microglobulin+. No correlation was found between the % DR+ cells and the level of lymphocyte stimulation. Separation of leukaemia cells on Ficoll gradients into fractions containing different proportions of DR+ cells did not correlate with LAD expression. Furthermore, antisera to DR antigens only partially blocked leukaemic LAD. The results support the notion that LAD on acute leukaemias are not necessarily associated with or identical to HLA-DR antigens, and that the lymphocyte activating capacity of HLA-DR may be modulated.

Full text

PDF
487

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albrechtsen D., Solheim B. G., Thorsey E. Antiserum inhibition of the mixed lymphocyte culture (MLC) interaction. Inhibitory effect of antibodies reactive with HLA-D-associated determinants. Cell Immunol. 1977 Feb;28(2):258–273. doi: 10.1016/0008-8749(77)90110-1. [DOI] [PubMed] [Google Scholar]
  2. Bodmer J. G. Ia antigens: definition of the HLA-DRw specificities. Br Med Bull. 1978 Sep;34(3):233–240. doi: 10.1093/oxfordjournals.bmb.a071503. [DOI] [PubMed] [Google Scholar]
  3. Bom-van Noorloos A. A., Schreuder I., de Groot-Swings G., Ubels-Postma J., von dem Borne A. E., Melief C. J. Variable MLC stimulatory capacity of neoplastic B and non-B/non-T lymphocytes expressing HLA-DR antigens. Tissue Antigens. 1982 Nov;20(5):352–363. doi: 10.1111/j.1399-0039.1982.tb02251.x. [DOI] [PubMed] [Google Scholar]
  4. Geier S. S., Cresswell P. Rabbit antisera to human B cell alloantigens: effects on the mixed lymphocyte response. Cell Immunol. 1977 Feb;28(2):341–354. doi: 10.1016/0008-8749(77)90117-4. [DOI] [PubMed] [Google Scholar]
  5. Greaves M., Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim Biophys Acta. 1978 Oct 27;516(2):193–230. doi: 10.1016/0304-419x(78)90008-2. [DOI] [PubMed] [Google Scholar]
  6. Han T., Dadey B., Minowada J. Stimulating capacity of fresh and cultured human leukaemic lymphoid and myeloid cells in 'one-way' mixed lymphocyte reaction. Immunology. 1977 Oct;33(4):543–551. [PMC free article] [PubMed] [Google Scholar]
  7. Han T., Minowada J. Use of stimulating capacity of mixed lymphocyte reaction (MLR-S) as a possible marker for the cell-origin of null-cell acute lymphoblastic leukaemia. Immunology. 1978 Aug;35(2):333–339. [PMC free article] [PubMed] [Google Scholar]
  8. Indiveri F., Wilson B. S., Russo C., Quaranta V., Pellegrino M. A., Ferrone S. Ia-like antigens on human T lymphocytes: relationship to other surface markers, role in mixed lymphocyte reactions, and structural profile. J Immunol. 1980 Dec;125(6):2673–2678. [PubMed] [Google Scholar]
  9. Janossy G., Goldstone A. H., Capellaro D., Greaves M. F., Kulenkampff J., Pippard M., Welsh K. Differentiation linked expression of p28,33 (Ia-like) structures on human leukaemic cells. Br J Haematol. 1977 Nov;37(3):391–402. doi: 10.1111/j.1365-2141.1977.tb01010.x. [DOI] [PubMed] [Google Scholar]
  10. Kuntz M. M., Innes J. B., Weksler M. E. Lymphocyte transformation induced by autologous cells. IV. Human T-lymphocyte proliferation induced by autologous or allogeneic non-T lymphocytes. J Exp Med. 1976 May 1;143(5):1042–1054. doi: 10.1084/jem.143.5.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Newman R. A., Greaves M. F. Characterization of HLA-DR antigens on leukaemic cells. Clin Exp Immunol. 1982 Oct;50(1):41–50. [PMC free article] [PubMed] [Google Scholar]
  12. O'Keefe D., Ashman L. Variation in accessory cell requirements in human mixed lymphocyte response to leukaemic cell lines. Immunology. 1982 Dec;47(4):633–641. [PMC free article] [PubMed] [Google Scholar]
  13. Opelz G., Kiuchi M., Takasugi M., Terasaki P. I. Autologous stimulation of human lymphocyte subpopulation. J Exp Med. 1975 Nov 1;142(5):1327–1333. doi: 10.1084/jem.142.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ostberg L., Lindblom J. B., Peterson P. A. Beta2-microglobulin on the cell surface. Specificity of inhibition of the mixed leukocyte reaction and mitogenic properties of antibodies against the two HLA antigen polypeptide chains. Eur J Immunol. 1976 Feb;6(2):108–113. doi: 10.1002/eji.1830060207. [DOI] [PubMed] [Google Scholar]
  15. Palacios R., Claesson L., Möller G., Peterson P. A., Möller E. The alpha chain, not the beta chain of HLA-DR antigens participates in activation of T cells in autologous mixed lymphocyte reaction. Immunogenetics. 1982;15(4):341–356. doi: 10.1007/BF00364258. [DOI] [PubMed] [Google Scholar]
  16. Reinsmoen N. L., Kersey J. H., Yunis E. J. Antigens associated with acute leukemia detected in the primed lymphocyte test. J Natl Cancer Inst. 1978 Mar;60(3):537–543. doi: 10.1093/jnci/60.3.537. [DOI] [PubMed] [Google Scholar]
  17. Romano P. J., Mann D. L. Specific MLC stimulation by cultured B cells. Tissue Antigens. 1976 Jul;8(1):9–12. doi: 10.1111/j.1399-0039.1976.tb00545.x. [DOI] [PubMed] [Google Scholar]
  18. Ross G. D., Jarowski C. I., Rabellino E. M., Winchester R. J. The sequential appearance of Ia-like antigens and two different complement receptors during the maturation of human neutrophils. J Exp Med. 1978 Mar 1;147(3):730–744. doi: 10.1084/jem.147.3.730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schlossman S. F., Chess L., Humphreys R. E., Strominger J. L. Distribution of Ia-like molecules on the surface of normal and leukemic human cells. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1288–1292. doi: 10.1073/pnas.73.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shackelford D. A., Kaufman J. F., Korman A. J., Strominger J. L. HLA-DR antigens: structure, separation of subpopulations, gene cloning and function. Immunol Rev. 1982;66:133–187. doi: 10.1111/j.1600-065x.1982.tb00437.x. [DOI] [PubMed] [Google Scholar]
  21. Snary D., Barnstable C. J., Bodmer W. F., Goodfellow P. N., Crumpton M. J. Cellular distrubtion, purification, and molecular nature of human Ia antigens. Scand J Immunol. 1977;6(5):439–452. doi: 10.1111/j.1365-3083.1977.tb02101.x. [DOI] [PubMed] [Google Scholar]
  22. Taylor G. M., Fergusson W. D., Harris R. Suppression of lymphoproliferative responses to alloantigens by autologous AML cells. Clin Exp Immunol. 1979 Jan;35(1):53–61. [PMC free article] [PubMed] [Google Scholar]
  23. Taylor G. M., Freeman C. B., Harris R. Response of remission lymphocytes to autochthonous leukaemic myeloblasts. Br J Cancer. 1976 May;33(5):501–511. doi: 10.1038/bjc.1976.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Taylor G. M., Jones S. V., Ridway J. C., Harris R. Stimulation of human lymphocytes in vitro by leucocytes from patients with untreated acute myeloid leukaemia. Clin Exp Immunol. 1977 Aug;29(2):229–239. [PMC free article] [PubMed] [Google Scholar]
  25. Van Voorhis W. C., Hair L. S., Steinman R. M., Kaplan G. Human dendritic cells. Enrichment and characterization from peripheral blood. J Exp Med. 1982 Apr 1;155(4):1172–1187. doi: 10.1084/jem.155.4.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wernet P., Betsch C., Barth P., Jaramillo S., Schunter F., Waller H. D., Wilms K. Human Ia alloantigens as cell-differentiation markers of normal and pathologic leukocyte surfaces. Scand J Immunol. 1977;6(5):563–574. doi: 10.1111/j.1365-3083.1977.tb02121.x. [DOI] [PubMed] [Google Scholar]
  27. Zier K. S., Huber C., Albert E., Braunsteiner H. Cell-mediated immune responses between HLA-identical siblings: recognition of antigenic changes associated with acute myelogenous leukaemia. Clin Exp Immunol. 1980 Apr;40(1):136–146. [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES