Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1985 Sep;52(3):347–354. doi: 10.1038/bjc.1985.200

Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.

J Cordell, T C Richardson, K A Pulford, A K Ghosh, K C Gatter, E Heyderman, D Y Mason
PMCID: PMC1977193  PMID: 3899155

Abstract

Two monoclonal murine antibodies have been raised against a delipidated extract of human cream. These antibodies were detected by immunohistological screening of hybridoma culture supernatants on sections of human breast tissue. One of those antibodies (E29) was subsequently screened against a range of normal and neoplastic human tissues and shown to react with a wide variety of human epithelia and with mesothelial cells. Antibody E29 was unreactive with other cell types, with the exception of occasional plasma cells. Antibody E29 is suitable for use on paraffin embedded tissue and represents a valuable reagent for the identification of tumours of epithelial origin.

Full text

PDF
352

Images in this article

350Figure 1
on p.350
351Figure 2
on p.351
351Figure 3
on p.351
352Figure 4
on p.352
352Figure 5
on p.352
352Figure 6
on p.352
352Figure 7
on p.352

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arklie J., Taylor-Papadimitrious J., Bodmer W., Egan M., Millis R. Differentiation antigens expressed by epithelial cells in the lactating breast are also detectable in breast cancers. Int J Cancer. 1981 Jul 15;28(1):23–29. doi: 10.1002/ijc.2910280105. [DOI] [PubMed] [Google Scholar]
  2. Burchell J., Durbin H., Taylor-Papadimitriou J. Complexity of expression of antigenic determinants, recognized by monoclonal antibodies HMFG-1 and HMFG-2, in normal and malignant human mammary epithelial cells. J Immunol. 1983 Jul;131(1):508–513. [PubMed] [Google Scholar]
  3. Ceriani R. L., Thompson K., Peterson J. A., Abraham S. Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule. Proc Natl Acad Sci U S A. 1977 Feb;74(2):582–586. doi: 10.1073/pnas.74.2.582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  5. Dearnaley D. P., Ormerod M. G., Sloane J. P., Lumley H., Imrie S., Jones M., Coombes R. C., Neville A. M. Detection of isolated mammary carcinoma cells in marrow of patients with primary breast cancer. J R Soc Med. 1983 May;76(5):359–364. doi: 10.1177/014107688307600506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Delsol G., Gatter K. C., Stein H., Erber W. N., Pulford K. A., Zinne K., Mason D. Y. Human lymphoid cells express epithelial membrane antigen. Implications for diagnosis of human neoplasms. Lancet. 1984 Nov 17;2(8412):1124–1129. doi: 10.1016/s0140-6736(84)91558-7. [DOI] [PubMed] [Google Scholar]
  7. Ellis I. O., Robins R. A., Elston C. W., Blamey R. W., Ferry B., Baldwin R. W. A monoclonal antibody, NCRC-11, raised to human breast carcinoma. 1. Production and immunohistological characterization. Histopathology. 1984 May;8(3):501–516. doi: 10.1111/j.1365-2559.1984.tb02360.x. [DOI] [PubMed] [Google Scholar]
  8. Foster C. S., Edwards P. A., Dinsdale E. A., Neville A. M. Monoclonal antibodies to the human mammary gland. I. Distribution of determinants in non-neoplastic mammary and extra mammary tissues. Virchows Arch A Pathol Anat Histol. 1982;394(3):279–293. doi: 10.1007/BF00430671. [DOI] [PubMed] [Google Scholar]
  9. Gatter K. C., Abdulaziz Z., Beverley P., Corvalan J. R., Ford C., Lane E. B., Mota M., Nash J. R., Pulford K., Stein H. Use of monoclonal antibodies for the histopathological diagnosis of human malignancy. J Clin Pathol. 1982 Nov;35(11):1253–1267. doi: 10.1136/jcp.35.11.1253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gatter K. C., Alcock C., Heryet A., Mason D. Y. Clinical importance of analysing malignant tumours of uncertain origin with immunohistological techniques. Lancet. 1985 Jun 8;1(8441):1302–1305. doi: 10.1016/s0140-6736(85)92794-1. [DOI] [PubMed] [Google Scholar]
  11. Heyderman E., Steele K., Ormerod M. G. A new antigen on the epithelial membrane: its immunoperoxidase localisation in normal and neoplastic tissue. J Clin Pathol. 1979 Jan;32(1):35–39. doi: 10.1136/jcp.32.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Ormerod M. G., Steele K., Westwood J. H., Mazzini M. N. Epithelial membrane antigen: partial purification, assay and properties. Br J Cancer. 1983 Oct;48(4):533–541. doi: 10.1038/bjc.1983.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Redding W. H., Coombes R. C., Monaghan P., Clink H. M., Imrie S. F., Dearnaley D. P., Ormerod M. G., Sloane J. P., Gazet J. C., Powles T. J. Detection of micrometastases in patients with primary breast cancer. Lancet. 1983 Dec 3;2(8362):1271–1274. doi: 10.1016/s0140-6736(83)91150-9. [DOI] [PubMed] [Google Scholar]
  15. Sloane J. P., Hughes F., Ormerod M. G. An assessment of the value of epithelial membrane antigen and other epithelial markers in solving diagnostic problems in tumour histopathology. Histochem J. 1983 Jul;15(7):645–654. doi: 10.1007/BF01002986. [DOI] [PubMed] [Google Scholar]
  16. Sloane J. P., Ormerod M. G., Carter R. L., Gusterson B. A., Foster C. S. An immunocytochemical study of the distribution of epithelial membrane antigen in normal and disordered squamous epithelium. Diagn Histopathol. 1982 Jan-Mar;5(1):11–17. [PubMed] [Google Scholar]
  17. Sloane J. P., Ormerod M. G. Distribution of epithelial membrane antigen in normal and neoplastic tissues and it value in diagnostic tumor pathology. Cancer. 1981 Apr 1;47(7):1786–1795. doi: 10.1002/1097-0142(19810401)47:7<1786::aid-cncr2820470711>3.0.co;2-8. [DOI] [PubMed] [Google Scholar]
  18. Sloane J. P., Ormerod M. G., Imrie S. F., Coombes R. C. The use of antisera to epithelial membrane antigen in detecting micrometastases in histological sections. Br J Cancer. 1980 Sep;42(3):392–398. doi: 10.1038/bjc.1980.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Taylor-Papadimitriou J., Burchell J., Moss F., Beverley P. Monoclonal antibodies to epithelial membrane antigen and human milk fat globule mucin define epitopes expressed on other molecules. Lancet. 1985 Feb 23;1(8426):458–458. doi: 10.1016/s0140-6736(85)91175-4. [DOI] [PubMed] [Google Scholar]
  20. To A., Coleman D. V., Dearnaley D. P., Ormerod M. G., Steele K., Neville A. M. Use of antisera to epithelial membrane antigen for the cytodiagnosis of malignancy in serous effusions. J Clin Pathol. 1981 Dec;34(12):1326–1332. doi: 10.1136/jcp.34.12.1326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. To A., Dearnaley D. P., Ormerod M. G., Canti G., Coleman D. V. Epithelial Membrane Antigen. Its use in the cytodiagnosis of malignancy in serous effusions. Am J Clin Pathol. 1982 Aug;78(2):214–219. doi: 10.1093/ajcp/78.2.214. [DOI] [PubMed] [Google Scholar]
  22. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wells C. A., Heryet A., Brochier J., Gatter K. C., Mason D. Y. The immunocytochemical detection of axillary micrometastases in breast cancer. Br J Cancer. 1984 Aug;50(2):193–197. doi: 10.1038/bjc.1984.162. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES